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K Voss

Publications and source records attributed to K Voss.

At least 55 records · Page 3Linked to original sources
PubMed1981

Age-related classification of pyramidal and stellate cells in the rat visual cortex: a Nissl study with the 'Morphoquant'.

(1) Using Nissl preparations and the automatic picture processing system 'MORPHOQUANT', VEB Carl Zeiss JENA, age-related classification of pyramidal and stellate cells of the layer IV in the rat's visual cortex was performed. (2) 12, 16, 20, 30, 90, and 420 days old animals were investigated. (3) Due to the high cell density and immature stage the neurons of an 8-day-old animal could not be measured. (4) A review is given about the automatic picture processing system 'MORPHOQUANT' and the applied computer program. (5) The analysis of one cell soma with the 'MORPHOQUANT' amounts to about 30 seconds. (6) The computer accepted 30 to 50% of the offered objects. The remaining ones could not be discriminated from the neighbourhood. (7) 14 standard features are registered and 7 statistically evaluated preferential taking into consideration area, form and texture of the objects. (8) 4 features are statistically relevant: soma area in picture points (KOFL) and whole extinction (EXTS) correspond to soma size; compactness (KOMP) and concentration of high grey values in the central part of the object (ZNTR) correspond to structural features of the soma, i.e. the structural density and the distribution patterns of strong coloured RNA and DNA particles. Form factors are not statistically relevant. (9) With increasing age the morphological features are clearer and the performance of the classificator is higher. (10) Soma size and structure of pyramidal and stellate cells develop similarly to an overshooting growth curve. The values reach the maximum approximately on postnatal day 16. (11) Age-related methodological and neurobiological problems are discussed.

Aging↗
PubMed1980

[The differential diagnosis of follicular adenomas and carcinomas in fine needle biopsies of the thyroid gland by means of automatic image analysis (author's transl)].

Problems in discriminating follicular adenomas from carcinomas in fine needle biopsy material of the thyroid gland by means of conventional microscopy led us to perform morphometric analyses. Applying a microscopic image analysis system for automatically recording of smears of the thyroid gland that were obtained from fine needle biopsies, it seemed possible to analyse "follicular neoplasias". However, in order to get statistical assurance, a larger number of samples remains to be investigated. The usefulness of the method is to be confirmed by its routine application.

Adenocarcinoma↗
PubMed1980

[Quantitative morphological investigations in livers and liver tumors after taking contraceptives (author's transl)].

Since the introduction of the orally applicable contraceptive steroids repeated reports have been published on the occurrence of side effects largely manifesting in the liver. By means of morphometrical and densitometrical evaluation of histological slide preparations of livers damaged by contraceptives (biopsies, operation material), qualitative and quantitative transitions between the individual degrees of the lesion up to malign cell transformation were registered by automated microscope picture analysis. It appeared that this cell transformation, like similar as in experimental hepatocarcinogenesis, is gradual process. Among the most remarkable cytopathological features are changes of nuclear and nucleolar size and of the sinusoid area and of the number of Kupffer's cells. The latter are clearly increased in hepatosis caused by contraceptives as compared with normal livers, they are reduced in the hepatocellular adenoma, and reach a value near zero in the carcinoma.

Adult↗
PubMed1979

Image processing in pathology. IX. A new morphometry system and its application on gangliocytes of the nucleus olivaris.

This paper describes a new software system for automatic image processing in pathology. The system is based on the hardware NU2/ODD/PDP8 and the thereby yielded results. The effectiveness of the system esp. its velocity has been essentially improved by a significant modification of the primary data sampling and by the new implementation of feature determination. The first application of this new system concerns age-dependent alterations of the gangliocytes in the nucleus olivaris.

Adolescent↗
PubMed1979

[Comparative, quantitative and morphological investigations (automatic image analysis--micromorphometric analysis) of myocardium of rat isoprenalin treatment (author's transl)].

The alterations of areas of myocardium of male rats (2, 4, and 6 months) are investigated after isoprenalin treatment (0.05 mg/kg body weight and day i.p. for 30 days). The investigations are carried out using automatic microscope picture analysis, morphometric point counting and resp. Both methods yield the same qualitative results: The effect of isoprenalin is dependent on age and yields for old rats (6 months) only very little morphometric alterations in the myocardium. The automatic microscope picture analysis is a very effective method of investigation (45 seconds/cross section).

Age Factors↗
PubMed1979

[Classification of nerve cell forms in lamina IV of the visual cortex of albino rats using Nissel preparation with the help of automatic picture processing].

1) Using the automatical picture processing device "MORPHOQUANT", VEB Carl Zeiss Jena, layer IV of the adult albino rat's area 17 was investigated in Nissl-preparations to classify pyramidal and stellate cells on the basis of quantitative features. 2) A review is given about the applied computer programme. 3) 30 seconds are necessary for adjustment, measurement and statistical calculation. 4) Five features per neuron soma were registered and statistical calculated: neuron area in picture points (KOFL), mean value of extinction (EXTM), total extinction (EXTS), shape (i.e. dia ratio, DMVH), and the distribution of strong coloured particles (i.e. centricity, ZNTR). 5) High statistical significance could be achieved only with regard to the neuron area and the distribution of strong coloured particles. 6) The causes for different results obtained in previous and present measurements and the importance of differentiation between several types of neurones are discussed as well.

Animals↗
PubMed1979

[Cytomorphometry of area 17 of the albino rat using the automatic picture processing].

Using the automatical picture processing, the layers II to IV of the adult albino-rat's area 17 were investigated in Nisselpreparations. Within 15 hours 14 features from approximately 10 000 neurons could be registered. Five features (neuron density = neuron number per 10 areas of measurement, mean value of neuron area in picture points, mean value of mean extinctions of neurons, whole extinctions of neurons, mean value of relative granula area of neurons) were statistically calculated and considered under several aspects: 1st Concerning the specifity of the layers very exact results could be obtained. 2nd In a detailed investigation of the lamina V pyramidal cells estimating somata areas no statistically significant classification could be achieved. 3rd There are slight structural changes of area 17 in rostrocaudal direction, but without statistical significance. Further investigations are necessary. 4th It is possible to differentiate between pyramidal cells and stellate cells of lamina IV. The numerical proportion between these two types of cells, however, deviates from individual estimations. Until now, this problem could not be satisfactorily be solved.

Animals↗
PubMed1978

Image processing in pathology.

The present study is an effort to ally biopsies to well defined groups of diseases by means of automatic analysis of liver cell nuclei. The results obtained should support the morphologic differential diagnosis of liver diseases. A total of 78 liver biopsies from patients with obstructive jaundice, chronic hepatitis, acute viral hepatitis or with alcoholic liver injury were investigated. Performing automatic analysis of the microscopic pictures 95% of the equatorial sectioned liver cell nuclei are correctly recognized.

Cell Nucleus↗
PubMed1978

Image processing in pathology. IV. Internal structure of mitochondria.

For extensive recording of mitochondrial membrane lengths hitherto statistical methods were applied which only provide mean values. Conventional measurements of the lengths by means of curvimetry or by intersection counts are required for estimating size distributions and secondary parameters. The former method requires expenditure in time, the latter yields only very rough values for single organelles. Automatic image processing is a possible way to obtain more informations. A method for an automatic definition of the internal mitochondria structure is given in this paper and preliminary results are discussed.

Computers↗
PubMed1978

Image processing in pathology. V. Interpretation of feature distributions of liver cell nuclei in histological sections.

A new method is applied for calculating the volume and extinction of liver cell nuclei from area and extinction of their sections in histological slices. With a special classificator for the automatical processing of microscopical pictures 96 per cent of morphologically controlled aequatorial sections of liver cell nuclei are correctly recognized. Using a mathematical model (additive fitting of three normal distributions to the given feature distribution) ploidie classes and volume classes can be recorded more exactly.

Cell Nucleus↗