PubMed Health⌕ Search

Biomedical subjects

K W Kaye

Publications and source records attributed to K W Kaye.

At least 37 records · Page 2Linked to original sources
PubMed1988

Modified high varicocelectomy: outpatient microsurgical procedure.

Fifty patients underwent modified high varicocelectomy as outpatients. Twelve of the 22 unilateral varicocelectomies were performed under local anesthesia. All patients tolerated the procedures well, and none required admission to the hospital. The modified high approach, which exposes the area above the internal inguinal ring and of the posterior spermatic cord, is straightforward and insures that both internal spermatic and cremasteric veins can be ligated. Use of the operating microscope prevents the inadvertent ligation of the testicular artery and lymphatics.

Adult↗
PubMed1988

Transrectal fine needle biopsy of the prostate: combined histological and cytological technique.

Transrectal fine needle biopsy was performed on 50 patients with suspicious prostatic examinations using the Surecut and Chiba needles. The 21 gauge Surecut needle provided a core of tissue for routine histology in 42 of 50 patients (84 per cent) as well as cells for cytology in 46 of 50 (92 per cent). This was in contrast to positive cytology results with the 22 gauge Chiba needle in 44 of 50 patients (88 per cent). Fourteen cancers were detected: 12 by Surecut histology, 11 by Surecut cytology and 10 by Chiba cytology testing, for sensitivity rates of 86, 79 and 71 per cent, respectively. Our results indicate that the 21 gauge Surecut needle provides cytology results equivalent to the 22 gauge Chiba needle. However, the Surecut needle has the added advantage of providing a core of tissue for routine histological study, and it can be used safely as an outpatient procedure on unprepared bowel.

Biopsy, Needle↗
PubMed1988

Spermatic vein embolization with hot contrast material: fertility results.

Spermatic venography with hot contrast material embolization was undertaken in 81 patients with varicoceles and infertility. Long-term follow-up information was available in 91% of the patients, and there was an overall conception rate of 40.5%. Embolization with hot contrast material was easily performed without special embolization devices and proved to be a safe and effective technique.

Adolescent↗
PubMed1988

Membrane-associated protein kinase activities in seminal plasma from vasectomized men.

Differential centrifugation was used to prepare heavy and light membrane fractions from the seminal plasma of vasectomized men. The two membrane fractions combined contained half of the phosvitin and histone kinase activities but only 7% of the total protein content in vasectomy semen. These two kinase activities as well as phosphorylation of endogenous membrane proteins were optimally stimulated by Mg2+; Mn2+ could effectively substitute for Mg2+ only in endogenous phosphorylation reactions. Neither the phosvitin nor histone kinase responded to cAMP or cGMP, but the histone kinase was strongly inhibited by the heat-stable cAMP-dependent protein kinase inhibitor. The phosvitin kinase was not affected by this inhibitor. The phosphorylation of endogenous proteins in the heavy membrane fraction was not affected by the protein kinase inhibitor but protein phosphorylation in the light membrane fraction was partly (45%) inhibited. The differential effects of increased ionic strength, sulphydryl protecting agents, and the protein kinase inhibitor on protein kinase activity towards lysine-rich histones, phosvitin and endogenous proteins, as well as differential extractability and binding to an anion exchange column of histone kinase and phosvitin kinase activities, indicate that more than one kinase activity is present in these membrane subfractions. Electron microscopic examination showed that there are several kinds of membrane-limited components in vasectomy seminal fluid that vary in size, density, and ultrastructure. The association of type(s) of protein kinase to individual membrane components remains to be established.

Cell Membrane↗
PubMed1986

Tamponade nephrostomy catheter for percutaneous nephrostolithotomy.

Hemorrhage from the nephrostomy tract is the most common major complication associated with percutaneous nephrostolithotomy. The tamponade nephrostomy catheter is a new addition to the endourologist's instruments; it is expressly designed to achieve immediate tamponade of the nephrostomy tract. The large-diameter, occlusive balloon (36F) is carried on a 14-F nephrostomy tube which is passed over a 5-F ureteral stent. As such, the catheter not only tamponades the nephrostomy tract but effectively drains the renal pelvis, while maintaining ureteral access.

Catheterization↗
PubMed1984

Deep posterior compartmental syndrome: a serious complication of the lithotomy position.

We describe a case of deep compartmental syndrome after use of the dorsolithotomy position. This condition is associated with significant morbidity unless recognized and treated immediately. This patient suffered a slight residual neuromuscular impairment. We review the pathogenesis, diagnosis, treatment and prevention of this condition, which in its early stages often mimics deep venous thrombosis.

Aged↗
PubMed1984

Detailed caliceal anatomy for endourology.

A thorough understanding of caliceal anatomy is required to interpret excretory urograms and to perform safely endourological manipulations. Caliceal angles, posterior rotation of the lateral renal margin and caliceal placement in relation to the lateral aspect of the body were studied in 26 right and 24 left kidneys. Differences between kidneys found to be Brödel's types from those of the Hodson type, and the significance of these findings for performing nephrostolithotomy are described.

Humans↗
PubMed1984

Protein kinase activities in human prostatic secretion: biochemical characterization and effect of prostatitis.

The enzymic properties of two protein phosphokinase activities in human prostatic secretion were determined with partially dephosphorylated phosvitin and lysine-rich histones as acceptor protein substrates. Both kinase activities had pH optima of 8.0 and required Mg2+. The histone kinase activity was stimulated by dithiothreitol and inhibited by increased ionic strength. Similarly, it was inhibited by the cAMP-dependent protein kinase inhibitor. MnCl2 and CaCl2 substituted poorly for MgCl2. In contrast, the phosvitin kinase activity was stimulated by increased ionic strength and inhibited by dithiothreitol. It was, however, unaffected by the cAMP-dependent protein kinase inhibitor. MnCl2 and CaCl2 substituted effectively for MgCl2. Both kinase activities were reduced 60% to 65% in prostatic fluids in men with chronic prostatitis.

Adenosine Triphosphate↗
PubMed1983

Comparison of Doppler examination and retrograde spermatic venography in the diagnosis of varicocele.

The accuracy of the Doppler stethoscope is compared with that of retrograde spermatic venography in the diagnosis of varicocele in 39 subfertile men. In the 38 cases in which left-sided venography was successful, there was a good correlation between the radiographic and Doppler results in 33 cases (87%). The five failures were all false-negative results in patients without clinically diagnosed varicoceles. In the 33 cases in which right-sided venography was successful, there was a good correlation between the radiographic and Doppler results in 17 patients (52%). This low sensitivity on the right side appears to be a minor drawback, because right-sided reflux almost always occurs in conjunction with left-sided reflux, and therefore varicocele would be diagnosed in these patients despite the false-negative Doppler finding. Unlike venography, the Doppler study is noninvasive, inexpensive, and easy to perform. We conclude that it should be part of all studies of varicocele and infertility and that it may prove sufficiently accurate for use in controlled studies of the effectiveness of varicocelectomy and the role of subclinical varicocele in the pathogenesis of infertility.

Humans↗
PubMed1983

Tissue sources of protein kinase activities in human seminal fluid: studies of normal, oligozoospermic, and vasectomized men.

Protein kinase activities in seminal fluids of normo-, hypo-, and oligozoospermic and vasectomized men were measured using lysine-rich histones and partially dephosphorylated phosvitin as acceptor substrates. There was a significant relationship of histone kinase but not phosvitin kinase activities with the number of spermatozoa originally present in the semen. Histone kinase and phosvitin kinase activities were diminished 88% and 62%, respectively, in vasectomy seminal fluid. The sex accessory gland sources of seminal fluid protein kinase activities not associated with spermatozoa were examined in split ejaculates of vasectomized men. Histone kinase activity was greater in the first fractions, suggesting that the prostate is its predominant contributor; whereas the distribution of phosvitin kinase activity did not indicate any preferential accessory gland source of this enzyme.

Humans↗
PubMed1983

Outpatient hydrocele and spermatocele repair under local anesthesia.

We performed 20 hydrocele repairs and 18 spermatocelectomies using Lord's techniques on outpatients under local anesthesia. The only complication was delayed wound healing early in the series when tight pressure dressings were used. We now use only a simple dressing and a scrotal support. This method for these operations reduces the cost by 74 per cent compared to inpatient procedures under general anesthesia. This safety and cost-effectiveness argue strongly for performance of most hydrocele repairs and spermatocelectomies as outpatient procedures under local anesthesia.

Adult↗
PubMed1983

Renal anatomy for endourologic stone removal.

A transparent kidney model that shows the position of the calices in relation to the surface can be fabricated easily and is invaluable in planning percutaneous nephrostolithotomies and other endourological procedures.

Humans↗
PubMed1983

Microsurgical vasovasostomy: an outpatient procedure under local anesthesia.

We performed microsurgical vasovasostomy on 25 outpatients using spermatic cord block anesthesia, an infrapubic incision and the 1 1/2-layer anastomosis technique. The average operating time was 2 hours 18 minutes and patients usually were discharged home after approximately 1 hour in the recovery room. Of the 21 patients who have had sperm counts performed 3 or more months postoperatively 19 have counts equal to or more than 20 million per ml., with an average motility of 43 per cent. Exclusive of the surgeon's fee, which is the same for inpatient and outpatient vasovasostomies, the average cost of the operation was +650, a savings of 41 per cent over the cost of an outpatient microsurgical vasovasostomy under general anesthesia and of 61 per cent over the cost of the procedure done on an inpatient basis under general anesthesia. The operation as described is safe and economical with minimal morbidity and has results comparable to those obtained with more complex and extensive vasovasostomy techniques.

Adult↗
PubMed1982

Presence and characterization of two protein kinase activities in human seminal fluid.

The presence of two protein kinase (ATP: protein phosphotransferase, EC 2.7.1.37) activities has been demonstrated in human seminal fluid, utilizing partially dephosphorylated phosvitin and lysine-rich histones as model acceptor substrates. Both kinase activities were maximal in the presence of MgCl2 and a sulfhydryl-protecting agent such as dithiothreitol; however, the histone kinase was stimulated to a greater extent by the latter. The histone kinase displayed a broad shoulder of activity at pH values of 7.1 to 7.6 with optimal activity at pH 8.0, and was inhibited by increased ionic strength (53% at 160 mM NaCl) and by the cyclic AMP-dependent protein inhibitor from rabbit muscle. The kinase activity towards phosvitin exhibited a broad pH profile with maximal activity at pH 7.2, was slightly stimulated by NaCl (20% at 160 mM), and was unaffected by the cyclic AMP-dependent protein kinase inhibitor. Kinetic studies revealed more than one apparent Km for the protein substrates and ATP. These differences in enzymic properties of kinase activities towards phosvitin and lysine-rich histones strongly indicate the presence of multiple enzymes. It appears that the histone kinase activity is attributable to the free catalytic subunit of a cyclic AMP-dependent enzyme. The protein kinase activities of seminal fluids from vasectomized men were 12-20% of those found for seminal fluids of normal men. This suggests that sperm may be a major source of protein kinase activities in seminal fluid.

Cations, Monovalent↗