Biomedical subjects
Publications and source records attributed to K WOLF.
A line of fibroblast cells has been established from tongue tissue of the bullfrog (Rana catesbeiana). The cells are near-triploid and were subcultured 57 times during the 2(2/3) years of their existence. Some of their characteristics are described.
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An established line of fish cells has been propagated in vitro for 21 months and 48 subcultivations. Important characteristics of the cells are described.
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Cold trypsin dispersion at pH 7.2 was used to obtain cultivable cells and cell groups from tissues of six species of fresh-water bony fishes, a frog, and a turtle. The cells readily attached to glass and were capable of at least limfted, and in some cases extended, division in media consisting of commercially available components.
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