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K Wenzelides

Publications and source records attributed to K Wenzelides.

13 recordsLinked to original sources
PubMed1992

Nucleolar organizer region-associated proteins in cancer cells. Quantitative investigations on gliomas, meningiomas, urinary bladder carcinomas and pleural lesions.

Using a modified silver staining technique, we investigated nucleolar organizer region-associated proteins (AgNORs) in paraffin sections of 156 neoplastic tissues and other lesions, including gliomas (n = 41), meningiomas (n = 20), urinary bladder carcinomas (n = 58), and neoplastic and reactive lesions of the mesothelium of the pleural cavity (n = 37). We found significant differences in the mean number and area of AgNORs per nucleus between nonanaplastic and anaplastic astrocytomas. In meningiomas AgNOR analysis may be useful to distinguish between mostly benign tumors (grade 1 tumors) and atypical ones. Urinary bladder carcinomas exhibited a statistically significant increase in both AgNOR number and area as the grade of malignancy increased. Diagnostically useful differences in the AgNOR configuration between inflammatory and neoplastic processes were found for mesothelial lesions. In general, a higher grade of malignancy correlated with an increase in the AgNOR number. This was accompanied by an increase in the total AgNOR area per nucleus, irrespective of whether the size of the individual AgNORs had changed.

Antigens, Nuclear↗
PubMed1991

A modified method for the detection of nucleolar organizer regions (AgNORs).

A modified method for the detection of argyrophilic nucleolar organizer regions (AgNORs) is described. By combination of the original silver staining with a Feulgen staining at the same specimen both the AgNORs and the nuclei of the cells under study are sufficiently contrasted, so that a partial automated counting and measurement of these structures can be done using a computerized microscope image analysis system.

Animals↗
PubMed1991

[Measurement of the number, size and location of AgNORs in histologic sections of astrocytomas and bladder carcinoma].

Nucleolus organizer regions (NORs) are associated with proliferative activity of tumors. An image analysis method is presented in this paper by which it is possible to analyze NOR location within the nucleus and relative to each other. Several features which characterized locations were measured in 20 urinary bladder carcinomas, eight astrocytomas, and two glioblastomas. Correlations were found to exist between those features, on the one hand, and tumor malignancy, on the other. They were particularly close in astrocytoma. Mean distance among NORs and closeness to the nuclear membrane were found to increase, as well, along with aggravating malignancy. As to the urinary bladder carcinomas examined, an unambiguous difference was found to exist between G 1 and G 1-2 carcinomas.

Astrocytoma↗
PubMed1991

[AgNORs in cells of urothelial carcinoma of the bladder. A quantitative study using automatic microscopic image analysis].

Biopsies taken from 52 cases of urinary bladder carcinoma together with samples collected from 6 controls were stained to visualize AgNORs, using a technique modified by the authors of this paper after its first introduction to histological practice by Ploton et al. (1986) as well as by Crocker and Nar (1987). AgNORs were quantitatively analyzed by means of an image processing system and a programme written by the authors' team for this particular purpose. The configuration was such that information was provided on the number and size of AgNORs. AgNORs were found to go up numerically along with aggravating malignancy, means values being 1.7/cell nucleus in Grade 1 urothelial carcinomas (controls being 1.6), 2.6 in Grade 2, and 3.3 in Grade 3. Mean AgNOR sum areas in the three above tumor groups were 3.4 microns 2, 7.4 microns 2, and 12.8 microns 2 (controls being 2.6 microns 2). Analysis of AgNORs, consequently, should provide prognostically relevant information helpful in more effective grading of urinary bladder carcinoma.

Carcinoma, Transitional Cell↗
PubMed1989

The PARTICLE expert system for tumor grading by automated image analysis.

While automated microscopic image analysis of histologic sections has been helpful in objectivizing histologic tumor grading and investigating the relationships between grading and prognosis, expert systems have the potential of linking image analysis and other data for statistical analysis and application to a wider range of tumors. One such expert system is PARTICLE, whose development was based on many years of experience in resolving histologic problems by image analysis. This paper discusses the philosophy of expert system for tumor grading and describes its implementation in the PARTICLE system. The system's structure, operations and applications are briefly presented. PARTICLE is essentially based on the evaluation of karyometric data.

Cell Nucleus↗
PubMed1989

Comparison of conventional morphometry and image analysis for the solution of histomorphometric problems.

The main differences between conventional morphometry and image analysis for their application in histopathology are pointed out. Conventional morphometry allows the use of contextural informations and a priori knowledge in a convenient manner. This is the most complicated thing in image analysis. The solution of this problem lies on the field of artificial intelligence. On the other hand, image analysis is able to measure densitometrical and textural features, to determine arbitrary quantiles and moments of feature distributions, to match scenes. Therefore the descriptive power of image analysis is much higher than the power of conventional morphometry.

Humans↗
PubMed1989

[The interactive image processing system AMBA/R].

Automated microscopic picture processing is a method of universal applicability useful in research and practice of medicine and biology but also in all areas of science and technology in which pictures are generated. The experience shows that good results can be achieved only through an effective picture processing system, effective in hardware and software. A description of such an effective system, the AMBA/R system, will be given in this paper.

Animals↗
PubMed1989

[Methods of characterizing histological sections based on the AMBA/R system].

There exist 2 characterization levels: karyometry and histometry. The state of the art in karyometry is relatively wide advanced. A wide spectrum of methods exists and there is often a good correlation between measured parameters and diagnosis as well as prognosis. The development of histometry is just in the first phase. This paper gives an introduction to the histometrical methods based on the AMBA/R-software-system.

Carcinoma↗
PubMed1989

[Grading of urinary bladder tumors by automated microscopic image analysis].

Studies were undertaken on biopsies from urinary papillomas and urothelial carcinomas of different grades (G1 to G3) along with normal urinary bladder mucosa from a total of 220 patients. They were performed by use of automated microscopic image analysis (system Robotron A 6471, software AMBA/R). As a result, the tumor grading was improved and lead to the separation of both papillomas and G1-carcinomas with high proliferative activity. In addition, it was possible to divide the G2-carcinomas into 2 groups which probably show a different biological behaviour.

Carcinoma, Transitional Cell↗
PubMed1979

[Comparative, quantitative and morphological investigations (automatic image analysis--micromorphometric analysis) of myocardium of rat isoprenalin treatment (author's transl)].

The alterations of areas of myocardium of male rats (2, 4, and 6 months) are investigated after isoprenalin treatment (0.05 mg/kg body weight and day i.p. for 30 days). The investigations are carried out using automatic microscope picture analysis, morphometric point counting and resp. Both methods yield the same qualitative results: The effect of isoprenalin is dependent on age and yields for old rats (6 months) only very little morphometric alterations in the myocardium. The automatic microscope picture analysis is a very effective method of investigation (45 seconds/cross section).

Age Factors↗
PubMed1978

Determination of infarct size in rats with 86rubidium.

A quick method is presented which allows the determination of infarct sizes within hearts of experimental animals like rats. The method is based on the drop of 86Rb uptake in infarcted hearts, and has been compared with infarct sizes determined morphometrically. The standard deviation of this method is low (6.5%) because an internal standard in used (noninfarcted myocard - usually right ventricle tissue - has been used).

Animals↗
PubMed1976

[Learning and chronobiological regulation following experimental damage to the coronary-artery wall of albino rats].

Forty male albino rats were used to study the relationship between experimentally (concentrated hydrochloric acid) produced local structural changes of the coronary artery wall and central nervous information processing and chronobiological events, respectively. The alterations of the coronary arteries caused by this treatment did not produce infarction but initiated on the whole length of the arterial branch proliferative processes that are complete within 12 weeks following the intervention. Central nervous information processing and chronoregulatory processes showed, immediately after exposure to hydrochloric acid, pronounced malfunctions followed by a stage of hyperreactivity (4 weeks following the damage). At 8 weeks from the intervention, the parameters of information processing and chronobiological events of the CNS again equalled the control values. Since the central nervous functions were back to normal earlier than the structural alterations, the results are interpreted as reflecting CNS-controlled interactions between the complex of visceral afference and efference, the regulation of which is determined by the CNS after coronary damage in such a way as to restore the adaptational capacity of the organism by a predominance of sanogenetic processes.

Animals↗