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Biomedical subjects

K Wlodarski

Publications and source records attributed to K Wlodarski.

At least 19 recordsLinked to original sources
PubMed2002

Osteogenic properties of various HeLa cell lines and the BMP family genes expression.

Heterotopic ossicles were induced in thigh muscles of immunosuppressed mice by implantation of suspension of several HeLa cell lines. These ossicles are the object of our research on osteoporosis. It was found that various HeLa cell lines differ in their potential to induce osteogenesis. In the previous paper we demonstrated the involvement of bone morphogenetic proteins (BMP-4 and BMP-6) secreted by HeLa cells in this phenomenon. In the present paper we try to find out the reason of the heterogeneity of various cell lines regarding the differences in their osteoinductive potencies. By the use of semiquantitative RT-PCR the differences in mRNA expression for several isoforms of BMP proteins in examined HeLa cell lines were found. The presence or absence of some of the BMP isoforms seems to be correlated with the quantity of heterotopically induced mineralised tissues. This was measured by weighing the deposited mineral after digestion of soft tissues surrounding the induced ossicles. This finding is supporting the thesis on high and uncontrolled heterogeneity of various HeLa cell lines used all over the world.

Animals↗
PubMed1984

Demonstration of virus particles in Moloney murine sarcoma virus-induced periosteal bone in mice.

Balb/c mice were inoculated intramuscularly with Moloney murine sarcoma virus in one of the hind legs. This led to the rapid development of a regressive sarcoma and also to the proliferation and osteogenic differentiation of cells in the adjacent periosteum. Examination of the tissues by transmission electron microscopy revealed the presence of type A and C virus particles within the sarcoma cells as well as within the cells of the newly formed bone. Extracellular type C virus particles were formed by budding from the cell surface and by release from disintegrating cells. No virus particles were found in the bone or the surrounding soft tissues of the contralateral, noninfected leg. These observations suggest that viral infection of periosteal cells are at least partly responsible for the osteogenic response associated with the virus-induced sarcoma. Production of growth factors by the sarcoma cells could also contribute to this process.

Animals↗
PubMed1982

Effects of nu gene on the numbers of mast cells in lymph nodes.

The total numbers of mast cells (MC) in popliteal lymph nodes of homozygous nude (nu/nu) and phenotypically normal, heterozygous (+/nu) BALB/c mice are greater than in normal (+/+) BALB/c mice at approximately 6 months of age. The values obtained from 4- to 7-month-old (nu/nu) and (+/nu) mice are twice as high as those from 2- to 3-month-old mice. It is concluded that the thymus does not directly determine the number of MC in lymph nodes, but the presence of the nu gene or another gene closely linked to the nu gene regulates the number of MC. 'Nude' mice infected with hepatitis virus accumulate many more MC than do healthy mice.

Aging↗
PubMed1979

Orthotopic bone induction at sites of Moloney murine sarcoma virus inoculation in mice.

Moloney murine sarcoma virus (M-MSV) induces at the site of inoculation in newborn and adult mice and rats various types of sarcoma, including osteosarcoma. The induced tumours are fatal in newborn, whereas sarcomas developed in adult animals regress spontaneously. The regression is mediated mainly by a cellular response. We have now demonstrated that the presence of sarcomas induced by M-MSV is a powerful stimulus for periosteal osteogenesis around tumour masses. Orthotopically induced osteogenesis by M-MSV may serve as a model for local regulation of bone growth and for cell differentiation studies, and may help explain the aetiology of some human bone disorders.

Animals↗
PubMed1976

Mast cells in the pinna of Balb/c 'nude' (nu/nu) and heterozygotes (ny/+)mice.

The relative number of mast cells in the ear lobes' skin (pinna) of nude (athymic) nu/nu and normal (thymic) nu/+ heterozygotes of Balb/c mice was similar. The results obtained contradict some suggestions about the general influence of the thymus on the number of mast cells in the skin and suggest the existence of some local factor(s) in regulation of skin mast cell numbers.

Animals↗
PubMed1976

Colony forming units (CFU) in bone marrow associated with heterotopic bone formation by epithelial cells grafted in mice.

The presence of bone marrow accompanying heterotopic bone induction in mice did not increase the number of endogenous spleen colonies. However, such bone marrow, as judged by the ability to form exogenous spleen colonies, contains colony-forming cells (CFC). No significant difference was found between the concentration of CFC in the induced and in the femoral bone marrow. This indicates a qualitative similarity between these two types of bone marrow inasfar as hemopoietic activity is concerned.

Animals↗
PubMed1975

Heterotopic chondrogenesis and osteogenesis induced by transformed cells: use of nude mice as a model system.

A subline of HeLa cells was shown to induce cartilage and bone formation in congenitally athymic (nude) mice when injected intramuscularly. The chondrogenesis and osteogenesis observed was similar to that found when other inducing epithelia are injected intramuscularly into cortisone-treated mice. A tumorigenic SV40 T antigen-positive human-mouse somatic cell hybrid with human chromosomes C7 and C6 did not induce cartilage or bone formation under similar conditions. The nude mouse may thus provide a system in which to investigate experimental host-cell differentiation without the complications of immunosuppression.

Animals↗