PubMed Health⌕ Search

Biomedical subjects

K X Zhou

Publications and source records attributed to K X Zhou.

4 recordsLinked to original sources
PubMed1994

[Effects of metalloproteinases-1 (TIMP-1) cDNA transfection on biological behaviors of PG cells].

A recombinant plasmid, which contains a full length cDNA of human tissue inhibitor of metalloproteinases-1 (TIMP-1), was constructed by using gene recombinant technique, and introduced into a highly metastatic human giant cell carcinoma (PG) by lipofectin technique. Two of the transfectants, PG-T2 and PG-T4, were studied thoroughly. Northern blot showed an increased level of TIMP-1 mRNA in PG-T2 and PG-T4, compared with PG and PG-MV1 (vector-transfected control). The two transfectants also exhibited higher TIMP-1 protein activities. Moreover, they showed significant reduction in their proliferation rate and invasive abilities. The abilities of forming colonies in soft agar and tumorigenecity in athymic nude mice were abrogated in PG-T2 and PG-T4. The preliminary results suggest that a specific upregulation of TIMP-1 expression in metastatic cells could not only suppress their invasive and metastatic phenotype, but also inhibit their proliferation and tumorigenecity.

Animals↗
PubMed1992

[The roles of T cells in experimental crescentic glomerulonephritis].

A crescentic glomerulonephritis (GN) model was induced by intravenous injection of rabbit anti-mouse glomerular basement membrane (GBM) antiserum and lipopolysaccharide (LPS) in BALB/c mice, heterozygous mice and nude mice respectively, in order to detect the T-cells effects on the development of crescentic GN. The immunofluorescence and morphological changes of glomeruli in different groups of animals were compared. Intense fluorescence (4+) of rabbit IgG could be found along the GBM in liner pattern in all the animals. Intense (3(+)-4+) mouse IgG was also found along the GBM in liner pattern in the normal and heterozygous mice, but could not be identified in the nude mice. The normal mice developed typical crescentic GN, characterized by serious degeneration and destruction of GBM, fibrin deposition and crescents formation, 3-6 weeks after the injection. The heterozygous mice only developed mild proliferation of the mesangial cells in the glomeruli but there was no glomerular lesion detected in the nude mice. It suggests that the glomerular immune damage requires the participation of functional T-cell.

Animals↗
PubMed1990

Correlation between T cells and experimental crescentic glomerulonephritis.

A model of crescentic glomerulonephritis (GN) in mice was induced by intravenous injection of rabbit anti-mouse glomerular basement membrane (GBM) antiserum and lipopolysaccharide. The procedure was carried out in BALB/c mice, heterozygous mice and nude mice. In order to examine the role of T cells in the pathogenesis of crescentic GN, immunofluorescent and morphologic changes in the glomeruli of these animals were studied. Intense (4+) linear deposition of rabbit IgG was found along the GBM of all test animals. Intense (3(+)-4+) linear deposition of mouse IgG along the GBM was present in normal and heterozygous mice, but not in nude mice. Normal mice developed typical crescentic GN characterized by severe degeneration and destruction of GBM, fibrin deposition and crescent formation 3-6 weeks after injection. Heterozygous mice only developed mild mesangial proliferation. No glomerular lesions were seen in nude mice. These preliminary data suggest that glomerular immunologic damage requires the participation of functional T cells, and that the induction of typical crescentic GN requires integral T-cell function.

Animals↗