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Biomedical subjects

K Yoshimura

Publications and source records attributed to K Yoshimura.

At least 505 records · Page 28Linked to original sources
PubMed1985

Complete amino acid sequence of a unique protein related to the variable domain of lambda light chain from a case with Fanconi syndrome.

The complete amino acid sequence has been determined of a unique protein from a 55-years-old female with multiple myeloma associated with Fanconi syndrome. It existed in a monomer form with an apparent molecular weight of 10K daltons, and was consisted of 106 amino acid residues. The sequence was characteristic of the V-region of lambda light chains and was highly homologous with that of the first 106 residues of V lambda III subgroup. The presence of an intact light chain as well as a 13K daltons fragment, corresponding to the entire C-region, strongly suggests that the unique component is a catabolic product from the intact light chain rather than an aberrant product of synthesis.

Amino Acid Sequence↗
PubMed1985

Effects of prostaglandin E1 and isosorbide dinitrate on acute hypoxic pulmonary vasoconstriction in conscious sheep.

The pulmonary vascular effects of prostaglandin E1 (PGE1) and isosorbide dinitrate (ISD) on acute hypoxic pulmonary vasoconstriction in conscious sheep were studied. While the animals inhaled room air or hypoxic gas (O2:N2 = 1:9), PGE1 (0.5 microgram/kg/min) and ISD (10 microgram/kg/min) were administered intravenously for 20 min, using an infusion pump. The changes of pulmonary arterial pressure (PPA) or pulmonary vascular resistance (PVR) in room air due to PGE1 were not significant, but in the hypoxic condition, PGE1 decreased PPA by 14.7% (p less than 0.05) and PVR by 14.5% (p less than 0.05). The effect of PGE1 in room air on systemic arterial pressure (PSA) remained nearly constant, whereas slight decreases in systemic vascular resistance (SVR) were noticed. In hypoxic condition, PSA levels were slightly decreased by PGE1, but SVR indicated a slight degree of delayed rise. When ISD in room air was administered, the values of PPA, PVR, PSA and SVR decreased by 9% (p less than 0.05), 10.4% (p less than 0.05), 17.7% (p less than 0.05) and 21.8% (p less than 0.05), respectively. In hypoxic condition, ISD decreased the values of PPA, PVR, PSA and SVR by 14.7% (p less than 0.05), 15.8% (p less than 0.05), 10.2% (p less than 0.05) and 6.7% (p less than 0.05), respectively. In summary in hypoxic condition both PGE1 and ISD cause similar decreases of PPA and PVR. Furthermore, there was a very little decrease in PSA during PGE1 infusion in the hypoxic condition.(ABSTRACT TRUNCATED AT 250 WORDS)

Alprostadil↗
PubMed1985

Augmentation of isoproterenol-stimulated tissue cyclic AMP level by cholinergic agonists in rat parotid gland.

It was found that methacholine and carbamylcholine, in addition to their known inhibitory effect, augmented the effect of isoproterenol on tissue cyclic AMP accumulation. The effect of methacholine was dose dependent, and significant augmentation was obtained at 0.1 microM with the maximum being attained at about 0.5 microM, whereas more than 10 microM were required to obtain the inhibitory effect. Atropine completely blocked the effect of methacholine. Similar augmentation of isoproterenol effect was obtained by oxotremorine and pilocarpine. Oxotremorine, however, did not inhibit the effect of isoproterenol. Difference in the effect between methacholine or carbamylcholine and oxotremorine was observed in their binding property to cholinergic receptors. A23187 augmented the effect of isoproterenol in a dose-dependent manner. Oxotremorine and A23187 augmented the effect of isoproterenol in the presence of isobutylmethylxanthine, but they did not augment the effect of forskolin and isobutylmethylxanthine on tissue cyclic AMP accumulation. Cholinergic agonist- and A23187-induced augmentation was abolished by omission of calcium in the medium. These results suggest that the augmentation is due to activation of adenylate cyclase, which is mediated by an increase in concentration of intracellular calcium.

1-Methyl-3-isobutylxanthine↗
PubMed1985

[Clinical study on tissue polypeptide antigen (TPA) in gastric cancer].

We evaluated whether assay of tissue polypeptide antigen (TPA) in the serum ia valuable for the determination of cancer stages compared to other tumor markers such as CEA, AFP, and ferritin. The study population consisted of 79 gastric cancer patients and 212 patients with benign gastroenteric disease. The percentage of positive cases for TPA (higher than 200u/l) was 41% in gastric cancer and 20% in active peptic ulcer. Serum TPA levels in well differentiated carcinoma and signet ring cell carcinoma were higher than that in other histological types. Serum TPA levels correlated well with the stage of the gastric cancer.

Adenocarcinoma↗
PubMed1985

[The distribution of cefotaxime in serum, lung lymph and lung tissue of sheep].

The levels of cefotaxime (CTX) in serum and lung lymph were compared in awake sheep which were prepared for collection of lung lymph. A single dose of 50 mg/kg of CTX was administered intravenously to sheep. The CTX level in serum or lung lymph reached a peak within 15 minutes after administration and decreased rapidly. The measurable concentration persisted for 240 minutes after administration. Ratios of lung lymph to serum concentration of CTX ranged from 1.0 to 1.2. In addition, the CTX levels in serum, lung lymph and tissue of right lower lobe were compared in anesthetized sheep to which CTX 50 mg/kg were given. Ratios of lung lymph and tissue in right lower lobe to serum concentration of CTX were 0.81 +/- 0.14, 0.06 +/- 0.02, respectively. From above results, CTX was well distributed in lung lymph. The levels of CTX in tissue of right lower lobe were markedly lower than those of lung lymph.

Animals↗
PubMed1985

Effect of organic and inorganic mercury compounds on the growth of incisor and tibia in rats.

The pharmacological effects of methyl mercury chloride (MMC) and mercuric chloride (HgCl2) (2-16 mg/Kg per day subcutaneously, for 6 d) upon the growth were studied in the incisors and proximal tibiae of immature rats histologically. Lead acetate was used as a time marker. 1. Mercury compounds slightly affected the body weight gains of the rats but apparently inhibited the longitudinal growth of proximal tibia and the effect increased with higher dosages. 2. Mercury compounds definitely inhibited not only the longitudinal growth (incisor growth) but also the appositional growth (dentin formation) of incisal dentin. 3. The inhibitory effect on the growth was ranked as follows: bone growth greater than dentin formation greater than incisor growth. 4. The actions of MMC on the growth of incisors and proximal tibiae appeared gradually and the response was biphasic; stimulatory and then inhibitory. The inhibitory effect appeared even after the injection was discontinued and appeared more extremely than during the injections. 5. In HgCl2 groups the inhibitory effect on the growth appeared rapidly. The effect increased with higher dosages and became stronger as the injections were repeated. However, this effect was weakened promptly after the injection was discontinued. 6. The repeated injections of mercury compounds hardly affected the level of serum calcium but disturbed the calcification of incisal dentin. From the above-mentioned results a possible mechanism was discussed. It is suggestive that MMC acts directly upon a cell and is transported into it. Once MMC was introduced into a cell it is slowly demethylated to inorganic Hg and acts as the demethylated mercury. When accumulated mercury is slight in volume, it stimulates and then inhibits the cell function with increasing mercury. However HgCl2 binds directly with an effector cell membrane in loose fashion. This may cause the ready reversibility of the effect.

Animals↗
PubMed1984

Prevalence of possible adult T-cell leukemia virus-carriers among volunteer blood donors in Japan: a nation-wide study.

A nation-wide survey of carriers of adult T-cell leukemia virus (ATLV) detected as anti-ATLA (ATLV-associated antigens) in volunteer blood donors was made in Japan. A total of 200 sera from donors between 40 and 64 years of age, collected in each of 64 blood centers located in eight districts, were tested for anti-ATLA by an indirect immunofluorescence test. The highest rate (8%) of seropositive donors was found in Kyushu and lower rates of 0.3 to 1.2% in other districts. However, the positive rate varied considerably in different localities even within districts with a high or low seropositive incidence. Further studies of all the donors (16 to 64 years old) in eight centers in Kyushu demonstrated two facts: (1) that the frequency of seropositive donors increased with age, and (2) that four centers in this district showed distinctly high incidences of seropositive donors, while the other four centers showed low incidences. The seropositive rate among all donors was estimated to be about 3% in Kyushu and 0.08 to 0.3% in all other districts.

Adolescent↗
PubMed1984

Lipid binding activities of the P2 protein in peripheral nerve myelin.

Lipid binding activities of the P2 protein in peripheral nerve myelin were examined using retinoic acid, retinol and oleic acid as ligands. The P2 protein showed the specific binding affinity to both of retinoic acid and retinol. The binding site of these ligands was suggested to be similar. In addition, the high binding activity of the P2 protein with oleic acid was also observed. The ligands specificities of the P2 protein are clearly different from those of cellular retinoic acid binding protein (CRABP), cellular retinol binding protein (CRBP), and Z protein. In amino terminal sequence, however, the P2 protein contained considerable homologous structure to these lipid binding proteins. Therefore, the P2 protein and these lipid binding proteins may belong to a family of structurally related proteins evolved from a common ancestral gene.

Amino Acid Sequence↗
PubMed1984

Determination of trace levels of fatty acid metal salts by high-performance liquid chromatography with fluorescence prelabeling.

A simple method is described for picomole determinations of fatty acid metal salts. Fatty acid salts are directly labeled with 4-bromomethyl-7-methoxycoumarin in the presence of excess ethylenediaminetetraacetic acid tripotassium salt without any solvent extractions. The fluorescence derivatives of fatty acids are separated by reverse-phase high-performance liquid chromatography followed by fluorometric detection. The response of each fatty acid (C8-C18) calcium salt is linear from 1 to 50 micrograms/ml of samples. The detection limit is about 7 pmol. Good recoveries are obtained for the calcium salts of myrystic acid and soap (C8-C18, C18:1,2). The new method is successfully applied to the study on biodegradation of fatty acids in river water.

Biodegradation, Environmental↗
PubMed1984

The prevalence of mental retardation (MR) in Kurume City.

For the purpose of early detection and intervention in mentally handicapped children, the mental retardation (MR) Monitoring Research Committee in Japan initiated a survey in 1981 to determine the prevalence rate and etiology of MR children who had been born between 1969 and 1974, that's those currently from 7 to 12 years of age, living in Kurume City. We found 154 MR children, and that the prevalence ratios in each year group ranged from 5.7/1,000 to 8.1/1,000. The mean prevalence of MR out of all children in each age year group was 7.1/1,000. Of the 154 MR children, 109 were males (9.8/1,000) and 45 females (4.3/1,000), giving a male excess ratio of 2.4:1. The sex difference was significant (p less than 0.005).

Adult↗