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K Zehethofer

Publications and source records attributed to K Zehethofer.

4 recordsLinked to original sources
PubMed1998

Phylogenetic relationships of Central European wolf spiders (Araneae: lycosidae) inferred from 12S ribosomal DNA sequences.

We have analyzed a sequence dataset of a portion of mitochondrial 12S rRNA gene of the ribosomal small subunit for 27 species of the family Lycosidae (wolf spiders) from Central Europe, belonging to six genera (Alopecosa, Arctosa, Pardosa, Pirata, Trochosa, and Xerolycosa) and four subfamilies (Evippinae, Lycosinae, Pardosinae and Venoniinae). Phylogenetic analyses were performed in two steps and corroborate the monophyly of all the genera analyzed with strong bootstrap support. In the first step focusing on the most ancestral splits the genus Pirata consistently emerged as the most ancestral branch, followed by the two genera Arctosa and Xerolycosa, with conflicting branching order, however. The second step of analysis placed Xerolycosa more ancestral than Arctosa. Arctosa appeared as sister group to the genera Alopecosa, Trochosa, and Pardosa. The palearctic genus Xerolycosa was not yet included in previous studies derived from morphological characters, but its placement based on mtDNA sequences is in good agreement to that according to current diagnostic morphological features. Further, the single representative of the genus Arctosa examined in our study was placed at a more ancestral position than in a previous investigation based on phenotypic characters. The superimposition of the currently used diagnostic phenotypic characters on the DNA-based phylogeny shows that both character sets are widely congruent; only 3 out of 16 phenotypic characters were resolved as homoplasious, suggesting their parallel evolution and/or reversal. Among the three different styles of predation found in the Lycosids, tube builders appear to be the most ancestral from which burrow dwellers descended and from which two groups of vagrant hunters evolved in parallel.

Animals↗
PubMed1995

[Population study in West Austria using DNA single locus probes].

Allele frequency distribution of the single-locus system YNH 24 (n = 302), G3 (n = 251), MS 43A (n = 333), and MS 31 (n = 333) was determined in Western Austria. After digestion with Hinf I, electrophoresis and Southern blotting, the genomic DNA was hybridised with the probes YNH 24, G3, MS 43A, and MS 31. Blood samples were taken from 333 unrelated caucasians living in the area of Innsbruck, Tyrol, Austria. The fragment distribution was calculated for each of the 4 single-locus systems. A single fragment pattern, indicating homozygosity, was shown in 9.93% with YNH 24, 12.45% with G3, 9.61% with MS 43A, and 7.21% with MS 31; the corresponding heterozygosity rates were 90.07%, 87.55%, 90.39%, and 92.79%, respectively. Our data are compared with those from England [18], Germany [3], Greenland [7], and Denmark [14]. Discrimination indices (Table 2) were calculated and statistical parameters (Table 3) presented.

Adult↗
PubMedDate not supplied

HLA-DQ alpha genotype and allele frequencies in an Austrian population.

The HLA-DQ alpha system is used to an increasing degree in forensic serology, for stain investigations, and paternity cases. Therefore samples which were taken from 85 unrelated persons living in Austria were examined. DNA was analyzed by polymerase chain reaction (PCR) amplification followed by hybridization to allele-specific oligonucleotide probes in a reversed dot-blot technique after extraction with phenol/chloroform. HLA-DQ alpha allele frequencies of German, Finnish, Dutch, British, Italian, Caucasian/USA and of Austrian populations were compared. Six common HLA-DQ alpha alleles are detectable. These alleles determine 21 possible genotypes. In our study 20 genotypes were obtained; the genotype 3/3 was not observed. The HLA-DQ alpha typing system using PCR-amplified DNA is a simple and rapid method for typing very small amounts of DNA and very old and/or degraded DNA. Therefore it is a helpful method in forensic casework.

Alleles↗