Biomedical subjects

Kaihua Lu

Publications and source records attributed to Kaihua Lu.

8 recordsLinked to original sources

PubMed2006

The role of bifidobacteria in gut barrier function after thermal injury in rats.

BACKGROUND: Early multiple organ dysfunction syndrome appears to be facilitated with bacterial translocation in severe burn injury, yet the mechanisms of bacterial translocation remain in dispute. The aim of this study was to characterize the potential role of intestinal bifidobacteria in the pathogenesis of gut-derived bacterial translocation after burns and to analyze the effects of bifidobacterial supplement on gut barrier function. METHODS: Wistar rats were randomly divided into burn group (Burn, n = 60), sham burn group (SB, n = 10) in experiment 1, and burn + saline group (BS, n = 30), burn + bifidobacteria group (BB, n = 30), and sham-burn + saline group (SS, n = 30) in experiment 2. Animals in BB group were fed bifidobacterial preparation (5 x 10(9) CFU/mL) after burns, 1.5 mL, twice daily. Animals in BS and SS were fed saline. Samples were taken on postburn days 1, 3, and 5. The incidence of bacterial translocation and counts of Bifidobacterium, fungi and Escherichia coli in gut mucosa, as well as the sIgA levels in mucus of the small intestine were determined. The positive sIgA expression in lamina propria and ileum mucosal injury were evaluated light microscopically by blinded examiners. RESULTS: The incidence of bacterial translocation was increased after burns, which was accompanied by significant decrease in number of bifidobacteria but significant increase in E. coli and fungi in gut mucosa, and elevation of levels of plasma endotoxin and IL-6 (p < 0.001). The incidence of bacterial translocation was markedly reduced after 3- and 5-day supplementation of bifidobacteria compared with control group (p < 0.05). The counts of mucosal bifidobacteria were increased by 4- to 40-fold, whereas E. coli and fungi were decreased by 2- to 30-fold and 10- to 150-fold, respectively, after bifidobacterial supplementation. The damage of mucosa tended to be less pronounced after 3-day bifidobacteria-supplemented formula compared with control group (grade 2 [0-6] versus grade 4 [3-6], p < 0.05). Moreover, the expression and release of sIgA was markedly augmented after 3-days of bifidobacteria-supplementation formula and it returned to normal range on postburn day 5. CONCLUSIONS: The decrease in counts and proportion of bifidobacteria to other flora in gut may play an important role in the development of bacterial translocation after thermal injury. Supplementation of exogenous bifidobacteria could improve gut barrier function, and attenuate bacterial/endotoxin translocation secondary to major burns.

Animal Feed↗

PubMed2006

[Rapid development of cosmetic medicine in China].

OBJECTIVE: To review and summarize the development during the last 20 years and the current status of cosmetic medicine, i.e., cosmetic surgery, in China, for the healthier development of this specialty in the future. METHODS: Literature concerned was reviewed, including conference abstracts, papers, and publications, and the present status and problems were analyzed. RESULTS: Cosmetic medicine was recognized as an independent specialty and gained its clear definition. The development of cosmetic medicine is an inevitable trend of the changing medical modules and the developing science and civilization. This trend fulfilled the need of the people. The related problems consisted of a high complication rate, confusion of management, and insufficient specific knowledge in part of the providers. CONCLUSION: The development of cosmetic medicine is an inevitable trend of the civilization development. For the healthy development of this specialty, scientific management and systemic education for the providers are crucial. Only those who have the plastic surgery background are able to participate in this practice.

China↗

PubMed2006

Mechanisms of dexamethasone to protect flaps from an ischemia-reperfusion injury.

OBJECTIVE: To study the effect of dexamethasone to protect flaps from an ischemia-reperfusion injury and elucidate its mechanism of regulating the death course of the neutrophils. METHODS: The rats were randomly divided into 3 groups. The vein of the rat was clamped for 8 h after the flap had formed. Group A: the normal flap; Group B: the saline control flap; Group C: the treatment flap with dexamethasone. The survival area of the flaps was measured at 7 days; the apoptotic and necrotic neutrophils, tumor necrosis factor alpha (TNF-alpha), and interleukin 10 (IL-10) concentrations were measured. RESULTS: The flap survival areas in Groups A and C were larger than those in Group B. The apoptotic neutrophils in Group B were fewer than those in Groups A and C on the 1st and 3rd days after operation; however, they were more in number in Group B than in groups A and C on the 6th day. The necrotic cells in Group B were more in number than those in Groups A and C. In Group B, the plasma TNF-alpha concentration reached the maximum level at 1 h, while the IL-10 level reached the lowest 3 h after the reperfusion. In Group C, the TNF-alpha concentration was lower than that in Group B and decreased dramatically at 6 h. The IL10 concentration was the lowest at 1 h, and increased rapidly at 3 h. Thus, ischemia-reperfusion could injure the flaps, probably through the abnormal action of the neutrophils, such as the disordered secretion of the cytokines and abnormal death course of the neutrophils. CONCLUSION: Dexamethasone can protect the flap from an ischemia-reperfusion injury by its regulation for the neutrophil function.

Animals↗

PubMed2002

[Rhinoplasty of unilateral cleft lip].

OBJECTIVE: To investigate the technique for unilateral cleft lip nasal deformities. METHODS: Through the traditional columella margin incision, the alar cartilage and the lateral nasal cartilage were exposed. After the mucosa between the two cartilages was divided, a mucosa-cartilage flap was developed and anchored, moving the lateral crus upwardly and medially, correcting the nasal deformity. RESULTS: 52 cases of primary nasal deformities and 18 cases of secondary nasal deformities were corrected with this technique. Satisfactory results were achieved. CONCLUSION: This technique is recommendable, which is more likely to restore the physiological characters of the nose than the conventional technique.

Adolescent↗

PubMed2002

[Investigation on contraction mechanism of expanded tissue].

OBJECTIVE: To investigate the contraction mechanism of expanded tissue. METHODS: White domestic pigs were employed to establish tissue expansion model. Tissue samples were taken after expansion for contraction measurements and histopathological observation. RESULTS: Dermis and capsule were the main positions of contraction. In the dermal layer, elastic fibers and myofibroblasts proliferated greatly and alpha-Actin enhanced in the myoepidermal cells and capillary endothelial cells during expansion. In the capsule, the direction of elastic and collagen fibers arranged crossly and formed into special contractible structure. CONCLUSION: Elastic fibers, myofibroblasts and alpha-Actin enhancement of cells were the substantial materials of tissue contracture increases. The results suggest that suitable tension must be applied into expanded flaps for anti-contraction.

Animals↗

PubMed2002

[Computer-aided tissue expansion 3D profilometry and surgery planning system].

OBJECTIVE: To develop and evaluate the computer-aided system in measurement of expanded skin and preoperative planning. METHODS: Stereophotogrammetric technique was used to gain the 3D image-pairs, from which the contours of the expanded sites were restored. The 3D surface data were provided to the specially developed "computer-aided tissue expansion 3D profilometry and surgery planning system", to calculate the expansion area and help the preoperative design. RESULTS: The system has been applied clinically in 16 tissue expansion sites of 11 patients with fairly good results since March 1999. Compared with the traditional method, this system is accurate, repeatable and feasible. CONCLUSION: This technique is useful and promising for improving the operation of tissue expansion.

Adult↗

PubMed2002

[The expanded distant skin flap and its application].

OBJECTIVE: To summarize and present the experiences and results of the expanded distant skin flaps used in plastic and reconstructive operations. METHODS: 102 patients who had undergone expanded distant skin flap transfer were reviewed and followed up. RESULTS: Good results were obtained. Especially when there was no sufficient normal skin close to the lesion or defect, the distant skin flaps were employed. The expanded distant skin flap can be directly transferred as a pedicle skin flap or an island skin flap. It can be indirectly transferred with an intermediary carrier or as a free skin flap. CONCLUSION: The expanded distant skin flaps have many advantages, indications, worthy of recommendation.

Humans↗

PubMed2002

[Comparison of ultrastructural changes of skin between continuous tissue expansion and conventional tissue expansion].

OBJECTIVE: To observe the ultrastrual changes of the skin between the continuous tissue expansion and the conventional tissue expansion. METHODS: Twelve white piglets were used for this study on an animal model of tissue expansion with the continuous tissue expansion group and the conventional tissue expansion group. The tissue samples in each group were harvested and prepared for the transmission electron microscope observation. RESULTS: The interspace among basal cells and spinose cells was increasing and the numbers of cell conjunctures were decreasing in the both groups. However, these changes in the continuous tissue expansion group were more obvious than in the conventional tissue expansion group. In the dermal layer of the skin, the ultrastructure of collagen fibers were basically normal. But, the fibroblasts and capillary endothelia cells were more activated in the continuous tissue expansion group, compared with the conventional tissue expansion group. The fibroblast apoptosis and collagenolysis spots were observed in both of the groups, while the red blood cells were also found in the tissue leaked outsides from the blood vascular cavities. CONCLUSION: Tissue expansion may result in tissue growth and tissue degeneration in the same time.

Animals↗