Biomedical subjects

Kaisheng Yan

Publications and source records attributed to Kaisheng Yan.

7 recordsLinked to original sources

PubMed2006

[Expression of perlecan in laryngeal carcinoma cell and its significance].

OBJECTIVE: To study the expression of perlecan in human laryngeal carcinoma cells and its significance. METHOD: The expression of perlecan mRNA in human laryngeal carcinoma cells, Hep-2 cells were investigated by using the techniques of semi-quantify RT-PCR. The expression of perlecan in the Hep-2 cells was investigated by using the techniques of immunohistochemistry. RESULT: It showed that the expression level of perlecan and perlecan mRNA significantly increased in Hep-2 cells as compared with the normal cells, Hacat cells (P < 0.01). CONCLUSION: These data raise the possibility that perlecan many play key roles in the growth,invasion and metastasis of human laryngeal carcinoma cells through either a paracrine or an autocrine mechanism.

Carcinoma, Squamous Cell↗

PubMed2006

[Experimental study of primary culture methods for spiral ganglion cells of newborn rats].

OBJECTIVE: To optimize culture condition of spiral ganglion cells (SGCs) in vitro and to obtain highly purified SGCs. METHOD: The spiral ganglions from newborn rats were digested in 0.25% trypsin and 0.001% DNase. The SGCs suspension was plated at a density of 10(9) cells/L. The cells were kept in serum free medium (DMEM/F12+B27 supplement). And 5 micromol/L cytosine arabinoside (Ara-C) was added to the medium on the second day and maintained for 48 hours. Serum medium with or without Ara-C was set as a control. The morphology and the purity of SGCs were observed under microscope. RESULT: Highly purified SGCs can be harvested in serum free medium (DMEM/F12+B27 supplement) with Ara-C. The SGCs were identified with mouse anti neurofilament protein antibody by immunohistochemistry methods. CONCLUSION: The method used in this study is an optimal means to culture and purify SGCs that can meet the needs of further study.

Animals↗

PubMed2006

[Application of rat tail collagen in the primary culture of rats marginal cells of stria vascularis].

OBJECTIVE: To study the feasibility of application of rat-tail collagen in the primary culture of rats marginal cells of stria vascularis. METHOD: The effect of self-made rat-tail collagen on the primary culture of rats marginal cells of stria vascularis was observed and estimated. RESULT: When cochlear stria vascularis fragment was isolated and cultured for 24 h, a few culture cells appeared around the fragments. About 48 to approximately 72 h later, clusters of culture cells could be seen,these cells showed the cobblestone shape under the microscope. The immunohistochemistry and the transmission electron microscopy showed the epithelial origination of these cells. CONCLUSION: It is feasible to the primarily culture the rats marginal cells of stria vascularis with self-made rat-tail collagen.

Animals↗

PubMed2006

[Excitotoxic effects of glutamate on the in vitro spiral ganglion cells].

OBJECTIVE: To explore the excitotoxic effects of glutamate on the in vitro primarily cultured spiral ganglion cell (SGC) of rat. METHOD: Spiral ganglion cells (SGCs) were cultured in vitro for 4 days, and exposed to 1 mmol/L glutamate for 24 hours. Damaged cells double-labeling with Hoechest33258 and PI were observed by fluorescence microscope. Ffluo-3 and CLSM for measurement of intracellular calcium levels were also utilized. RESULT: Most cells were damaged and the intracellular calcium increased and after exposure to 1 mmol/L glutamate (P < 0.05). CONCLUSION: Glutamate excitotoxicity was associated with free intracellular calcium ion concentration elevation in SGC.

Animals↗

PubMed2006

[Neurofibromatosis type 2].

OBJECTIVE: To recognise the predisposing factors, clinical manifestations, diagnosis and treatment of neurofibromatosis type 2 (NF2). METHOD: The clinical data of one NF2 case was reported and the literatures were also reviewed. RESULT: The patient was diagnosed at a much later stage than onset. Progressive hearing loss and tinnitus were the initial symptoms. MRI scan indicated space-occupying lesions in the bilateral cerebellopontine angles, bilateral cavernous sinuses and bilateral cervical parts of the patient. The patient was diagnosed as NF2 according to the National Institutes of Health (NIH) criteria, and received operation on the left acoustic tumor. The tumor was proved to be schwannomas by pathological test. The hearing loss and the facial nerve paralysis (House-Brackmann II) had appeared after operation. CONCLUSION: NF2 is an autosomal dominant, highly penetrant disease which is characterized by bilateral vestibular schwannomas. Early diagnosis and management for tumor is very important for survival and hearing preservation. The "golden standard" in terms of diagnostic precision is the magnetic resonance imaging (MRI) scan with gadolinium enhancement.

Adult↗

PubMed2006

[Suppression of proliferation of Hep-2 cells by stable expression of anti-sense perlecan cDNA].

OBJECTIVE: To study the suppression of proliferation of Hep-2 cells by stable expression of anti-sense perlecan cDNA. METHOD: In this study, the plasmids of recombination eukaryotic expression vector perlecan anti-sense cDNA (pAP) were transfected into Hep-2 cells by using cationic liposome (lipofectamine 2000) and divided into three groups: non-transfected group, WT group; transfection with no load carrier, neo group; and transfection with the pAP plasmid, pAP group. Semi quantify RT-PCR, western blot assay and MTT assay were used to detected the expression of perlecan mRNA and protein in the three groups; the level of cell proliferation; and the responsivity of basic fibroblast growth factor (bFGF). RESULT: It was showed that the expression of perlecan mRNA and protein were significantly reduced in the pAP group compared with WT group and ph beta Apr-neol transfected group ( P < 0.01). In the presence of 1 microg/L of bFGF in low serum (0.1% FCS), the pAP transfected cells showed a reduced proliferation rate (MTT assay) while the wild type cells and ph beta Apr-neol transfected cells grew rapidly. CONCLUSION: The growth of Hep-2 cells could be inhibited significantly by perlecan anti-sense cDNA plasmids transfection.

Apoptosis↗

PubMed2005

[Expression of vascular endothelial growth factor and its receptors in laryngeal carcinoma cell and its significance].

OBJECTIVE: To study the expression of vascular endothelial growth factor (VEGF) and both the Flt-1 and KDR high affinity VEGF receptors in human laryngeal carcinoma cells and its significance. METHOD: In this study, we investigated the expression of VEGF mRNA and both the Fit-1 mRNA and KDR mRNA high affinity VEGF receptors in human laryngeal carcinoma cells using techniques of semi-quantify RT-PCR. RESULT: It was showed that the expression level of VEGF mRNA was significantly increased in human laryngeal carcinoma cells as compared with the normal cells, Hacat cells (P <0.05), and there was the high expression level of Flt-1 mRNA in the Hep-2 cells, but not KDR mRNA. CONCLUSION: These data raise the possibility that VEGF and its receptors many play key roles in the growth, invasion and metastasis of human laryngeal carcinoma cells through either a paracrine or an autocrine mechanism.

Cell Line, Tumor↗