Biomedical subjects
Kathleen Cooper
Publications and source records attributed to Kathleen Cooper.
Microarray screening of lymphocyte gene expression differences in a multiplex schizophrenia pedigree.
In order to help prioritize the selection of candidate genes and to study possible trait and not state related changes in gene expression, we compared lymphocytic gene expression patterns of five individual family members with schizophrenia and nine unaffected individuals from a large multiplex high density pedigree. We screened gene expression by microarray consisting of 1128 brain focused genes. Three criteria for selection of microarray gene differences between schizophrenia and unaffected family members were employed: a significant t-test, expression in a majority of subjects, and fold change magnitude. Gene expression levels were significantly different for nine genes between individuals with schizophrenia compared to unaffected controls, and two genes were validated by real-time PCR. The expression of the neuropeptide Y receptor Y1 gene (NPY1R localized at 4q31.3-q32) and the human guanine nucleotide-binding regulatory protein Go-alpha (GNAO1 localized at 16q13) was significantly decreased in individuals with schizophrenia compared to unaffected family controls by microarray and real-time PCR. The cytosolic malate dehydrogenase gene (MDH1 localized at 2p13.3) was also significantly increased by microarray analysis and showed a trend for increase by real-time PCR. The significant genes are discussed in terms of proximity to linkage regions, prior association studies of schizophrenia, and other reports of microarray screening of schizophrenia tissue. Evidence from these studies taken together with the present study suggests critical pathways in schizophrenia may be studied in peripheral tissue as part of the strategy in functional genomic convergence. This preliminary study needs to be repeated by screening a larger set of genes in additional families with schizophrenia. The present study offers support for examination of gene expression patterns using lymphocytic RNA for complex neuropsychiatric disorders from large cohorts of patients.
Just whose autonomy is it?
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Improving the health and well-being of adolescent boys.
Adolescent men are at risk of having significant unmet health care needs. Like adolescent girls, they have complex health care needs and are more likely than younger children to be to be uninsured. They are less likely than women and other age groups to seek medical attention from traditional sources of care. Because of inadequate youth-oriented services, as well as teens' developmental stage, they have a tendency to receive care that is brief and problem oriented [20]. Such care is not likely to address complex problems that may be related to risk behaviors. Adolescent boys are also more concerned with the skills of the provider offering services than with the system in which the provider functions. Opportunities for outreach to adolescent men exist within many institutions. Nurses as advocates, educators, counselors, and providers of preventive health care have a creative opportunity for enhancing services to the teenage boy. The school is a natural place to begin as adolescents spend a significant part of each day there. Family planning and STI clinics are a source of care that are not well used by adolescent males, but when they do attend it is an opportunity to identify problems, provide counseling and referrals, and offer continuity. These health care institutions are not often welcoming or comfortable for the male youth. Use of these clinics will be enhanced by providers demonstrating increased acceptance of the adolescent when he attends as well as actively requesting that he attend with his partner. The most unusual but sorely needed outreach must be made to incarcerated and delinquent adolescent male. Residential facilities for delinquent youth need to be encouraged to provide a multidisciplinary comprehensive medical, mental health, and social services model. This approach will not only benefit the adolescent but the youth's community as well. Emergency rooms represent another crucial, missed opportunity to connect with young men. With some forethought and follow-up, emergency departments must become connected to appropriate and accessible adolescent primary care resources in the community. Finally, adolescent men are an underinsured population. Nurses can be important advocates of available state insurance funds, sliding scale or free services to youth, and must be a voice in youth policy-making.