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Kazuhiro Koike

Publications and source records attributed to Kazuhiro Koike.

2 recordsLinked to original sources

In vivo three-dimensional patellar tracking on the femur.

In vivo three-dimensional patellar tracking under weightbearing conditions was investigated with the principal reference axes of the femur in the coronal and axial planes, using a biplanar image-matching technique. Three-dimensional knee models of eight healthy volunteers were constructed using computed tomography scanning. Projection images of the models were fitted onto anteroposterior and lateral radiographs of the knees at hyperextension and at every 15 degrees from 0 degrees to 120 degrees flexion. Knee motion then was reconstructed on a computer. Patellar tracking during knee flexion was described simply with a medial jerk shift of 8 mm in early flexion until 30 degrees and a linear tracking with minimal mediolateral translation. The linear tracking portion was located laterally 5 mm from the mediolateral center of the femoral condyles. On average, the direction of this linear tracking was almost perpendicular to the distal condylar line in the coronal plane and perpendicular to the posterior condylar axis in the axial plane. These results help improve the understanding of patellofemoral kinematics and provide useful information for the design and positioning of the prostheses used in total knee arthroplasty.

Adult↗

A comprehensive rice transcript map containing 6591 expressed sequence tag sites.

To determine the chromosomal positions of expressed rice genes, we have performed an expressed sequence tag (EST) mapping project by polymerase chain reaction-based yeast artificial chromosome (YAC) screening. Specific primers designed from 6713 unique EST sequences derived from 19 cDNA libraries were screened on 4387 YAC clones and used for map construction in combination with genetic analysis. Here, we describe the establishment of a comprehensive YAC-based rice transcript map that contains 6591 EST sites and covers 80.8% of the rice genome. Chromosomes 1, 2, and 3 have relatively high EST densities, approximately twice those of chromosomes 11 and 12, and contain 41% of the total EST sites on the map. Most of the EST-dense regions are distributed on the distal regions of each chromosome arm. Genomic regions flanking the centromeres for most of the chromosomes have lower EST density. Recombination frequency in these regions is suppressed significantly. Our EST mapping also shows that 40% of the assigned ESTs occupy only approximately 21% of the entire genome. The rice transcript map has been a valuable resource for genetic study, gene isolation, and genome sequencing at the Rice Genome Research Program and should become an important tool for comparative analysis of chromosome structure and evolution among the cereals.

Chromosome Mapping↗