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Kazuyuki Hyodo

Publications and source records attributed to Kazuyuki Hyodo.

9 recordsLinked to original sources

Hard-x-ray region tomographic reconstruction of the refractive-index gradient vector field: imaging principles and comparisons with diffraction-enhanced-imaging-based computed tomography.

The unique tomographic imaging method based on refractive effects that was recently developed by Maksimenko et al. [Appl. Phys. Lett. 86, 124105 (2005)] exhibits an excellent imaging property in the hard-x-ray region for phase objects such as soft materials and biological samples. However, there seems to have been little consideration of the physical aspects of the underlying imaging principles. Also, as the method is similar to diffraction-enhanced-imaging (DEI)-based computed tomography (CT), the difference between these two methodologies has not been made clear. We theoretically consider the imaging principles starting from the measurement process to the reconstruction procedures from the viewpoint of geometrical optics and then clarify their difference in relationship to the physical quantities to be depicted. The major feature of this novel method is the in-plane two-dimensional vector-field reconstruction of the refractive-index gradient in an object, while DEI CT obtains the out-of-plane scalar-field gradient component. In other words, the novel method and DEI CT present the transverse and the longitudinal components, respectively, of the three-dimensional vector fields of the gradient refractive index. Therefore they can be considered complementary to each other.

Algorithms↗

First application of X-ray refraction-based computed tomography to a biomedical object.

We have developed X-ray refraction-based computed tomography (CT) that is able to visualize soft tissue in between hard tissue. The experimental system consists of Si(220) diffraction double-crystals and is called the DEI (diffraction-enhanced imaging) method, in which the object is located between the crystals and a CCD camera to acquire data as 360 X-ray images. The X-ray energy used was 17.5 keV. The algorithm used to reconstruct CT images was developed by A. Maksimenko and colleagues. We successfully visualized articular cartilage and the distribution of bone marrow, which are inner structures. Our method has much higher contrast compared to the conventional absorption-based CT system.

Algorithms↗

High-energy phase-contrast X-ray imaging using a two-crystal X-ray interferometer.

To broaden the scope of phase-contrast X-ray imaging in biomedical applications, the energy of X-rays in a large-area imaging system using a two-crystal X-ray interferometer has been increased from 17.7 keV to 35 keV. The use of higher-energy X-rays makes it possible to observe larger samples having greater variation in density within shorter measurement periods, at higher spatial resolution, and at lower X-ray doses. After optimizing the imaging system for 35 keV X-rays, a 25 mm x 30 mm interference pattern with 50% visibility was generated at the Photon Factory. The system's capabilities are demonstrated by this pattern and a high-quality three-dimensional image of a rat heart.

Animals↗

Coherence-contrast x-ray imaging based on x-ray interferometry.

Coherence-contrast x-ray imaging--which detects changes in the degree of coherence caused by the placement of a sample in an x-ray interferometer--was developed for biomedical applications. Because the technique's sensitivity depends on the density gradient in the sample, it is particularly suitable for observing biomedical samples with large density differences, such as samples that include both biological soft tissue and bone. A measurement principle and method of this technique are described, and a fine coherence-contrast image of a mouse leg is given as an example result.

Animals↗

Electron density measurement with dual-energy x-ray CT using synchrotron radiation.

Monochromatic x-ray computed tomography (CT) at two different energies provides information about electron density of human tissue without ambiguity due to the beam hardening effect. This information makes the treatment planning for proton and heavy-ion radiotherapy more precise. We have started a feasibility study on dual energy x-ray CT by using synchrotron radiation. A translation-rotation scanning CT system was developed for quantitative measurement in order to clarify what precision in the measurement was achieved. Liquid samples of solutions of K2HPO4 and solid samples of tissue equivalent materials were used to simulate human tissue. The experiments were carried out using monochromatic x-rays with energies of 40, 70 and 80 keV produced by monochromatizing synchrotron radiation. The solid samples were also measured in a complementary method using high-energy carbon beams to evaluate the electron densities. The measured electron densities were compared with the theoretical values or the values measured in the complementary method. It was found that these values were in agreement in 0.9% on average. Effective atomic numbers were obtained as well from dual-energy x-ray CT. The tomographic image based on each of the electron densities and the effective atomic number presents a different feature of the material, and its contrast drastically differs from that in a conventional CT image.

Connective Tissue↗

Large-area phase-contrast X-ray imaging using a two-crystal X-ray interferometer.

Large-area (25 x 20 mm) phase-contrast X-ray imaging was attained by using a skew-symmetric two-crystal X-ray interferometer. The sub-nrad angular control required to operate the X-ray interferometer was achieved with a sleeve bearing and a feedback-positioning system. As a demonstration, measurements of a phase map of a rat's liver and a phase-contrast tomographic three-dimensional image of a piece of a rabbit's liver were performed at the Photon Factory using 0.07 nm synchrotron radiation X-rays.

Animals↗

Inhomogeneous vasodilatory responses of rat tail arteries to heat stress: evaluation by synchrotron radiation microangiography.

Tail blood flow is crucial for dissipating body heat in rats. Angiographies are convenient tools to evaluate tail circulation. However, conventional angiographies do not have sufficient sensitivity or spatial resolution for small vessels. Recently, we developed a novel microangiographic system using monochromatic synchrotron radiation and a high-definition video camera system. Here, we report an evaluation of rat tail circulation under heat stress using the synchrotron radiation microangiographic system. We performed an experiment using the microangiography of the caudal artery before and after heating up WKAH/HkmSlc rats to rectal temperature of 39 degrees C. The images were digitized and temporal subtraction was performed, and the diameters of caudal arteries were evaluated. After heating, the medial caudal artery was markedly dilated (320 +/- 53 to 853 +/- 243 micro m in diameter, p<0.001), while no significant change was observed in the lateral caudal arteries (139 +/- 42 to 167 +/- 73 micro m) and segmental anastomosing vessels. The heat stress allowed for visualization of the superficial caudal arteries with a diameter of approximately 60 micro m, not visible prior to heating. Thus, synchrotron radiation microangiography demonstrated that the rat tail possessed dual sets of arteries; one set was highly sensitive to heat-induced vasodilation (medial caudal artery and superficial caudal arteries) and the other set was less sensitive (lateral caudal arteries and segmental anastomosing vessels).

Angiography↗

[Refraction-contrast bone imaging using synchrotron radiation].

The X-ray refraction-contrast imaging using synchrotron radiation with some X-ray energies is successfully performed at B120B2 of SPring-8. The refraction-contrast images of bone samples such as human dried proximal phalanx, wrist, upper cervical vertebrae and sella turcica, and as mouse proximal femur, using the synchrotron X-ray are always better in image contrast and resolution than those of the absorption-contrast images using the synchrotron X-ray and/or the conventional X-ray tube. There is much likeness in the image contrast and resolution of trabeculae bone in the human dried proximal phalanx between X-ray energy of 30 keV at sample-to-film distance of 1m and those of 40, 50 keV at those of 4,5m, respectively. High-energy refraction-constrast imaging with suitable sample-to-film distance could reduce the exposure dose in human imaging. In the refraction-contrast imaging of human wrist, upper cervical vertebrae, sella turcica and mouse proximal femur using the synchrotoron X-ray, we can obtain better image contrast and resolution to correctly extract morphological information for diagnosis corresponding to each of the clinical field than those of the absorption-contrast images.

Animals↗

[Phase-contrast x-ray imaging with x-ray interferometer for medical applications].

Phase-contrast x-ray imaging with x-ray interferometer can depict the minute difference within the biological object, and its sensitivity is about 1000 times higher than that of absorption-contrast method. For biomedical use of this technique, a large monolithic x-ray interferometer and 2 crystal interferometer having a field of view with 25 mm x 25 mm is being developed. Phase-contrast x-ray CT could reveal detail structures within tumor and surrounded tissue, and the vessel imaging of rat liver is also possible using physiological saline at 17.7 keV x-ray energy. Recently, human breast tissues were imaged at 35 keV and the contrast of image was much better than usual absorption contrast x-ray image obtained at 17.7 keV energy.

Animals↗