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Ke-Feng Dou

Publications and source records attributed to Ke-Feng Dou.

25 records · Page 2Linked to original sources
PubMed2003

Palliative surgery for hilar cholangiocarcinoma.

OBJECTIVE: To evaluate the results of palliative surgical treatment of hilar cholangiocarcinoma in terms of quality of life, survival period and cholangitis rate. METHODS: The clinical data on 232 patients with hilar cholangiocarcinoma in the last 22 years were analyzed retrospectively. Palliative operations included extrahepatic or intrahepatic choledochojejunostomy (123 patients), bridge internal drainage (15), endoscopic biliary drainage (49), percutaneous transhepatic biliary drainage or celiotomy biliary drainage (29), and exploratory celiotomy external drainage (16). RESULTS: In this series, the operative mortality rate was 9.1%, and no significant difference was observed between groups. The rate of cholangitis after operation was significantly lower in Roux-en-Y choledochojejunostomy group (16.2%) and bridge internal drainage group (15.4%) than in internal drainage group (35.5%, P<0.01), including percutaneous transhepatic biliary drainage (PTBD), endoscopic retrograde biliary drainage (ERBD), and celiotomy (or PTBD) external biliary drainage group (39.1%, P<0.01). No significant difference in survival was observed between the Roux-en-Y choledochojejunostomy group (9.3+/-1.8 months) and PTBD (or ERBD) internal drainage group (8.7+/- 2.2 months), but the survivals of the above groups were significantly longer than those of the bridge internal drainage group (6.5+/-1.7 months, P<0.05) and celiotomy (or PTBD) external biliary drainage group (4.4+/-2.1 months, P<0.01). CONCLUSIONS: In unresectable cholangiocarcinomas, either operative bilioenteric bypass or percutaneous transhepatic biliary drainage can achieve significant palliation. Roux-en-Y choledochojejunostomy is the best choice for palliative operation. The use of U-tube is recommended for internal radiation therapy.

Adult↗
PubMed2003

[Effect of granulocyte colony-stimulating factor on the ratio of peripheral blood dendritic cell subsets].

AIM: To clarify the effect of granulocyte colony-stimulating factor (G-CSF) on the radio of two peripheral blood dendritic cell(DC) subsets in-vivo. METHODS: Various doses (0, 5, 10 and 15 microg) of G-CSF were subcutaneosly injected respectively into BALB/c mice, once each day, for 6 days running. Six days later, DCs were separated from peripheral blood. Then the flow cytometry was used to detect the radio of DC1 and DC2 (CD11c(+) CD8a(-) and CD11c(+) CD8a(+)), and to calculate their absolute numbers. RESULTS: After stimulation with varying doses of G-CSF for 6 days, the absolute number of DC2s increased from 9.6x10(6)/L to 55.1x10(6)/L (P<0.01), while that of DC1s had no notably variation, so the ratio of DC1 and DC2 decreases from 4.2+/-1.1 to 0.7+/-0.3 (P<0.01). CONCLUSION: G-CSF can increase the absolute number of peripheral blood DC2s, but has no influence on peripheral blood DC1 number, thus inversing the ratio of DC1/DC2 in-vivo.

Animals↗
PubMed2003

[Induction of apoptosis of HepG2 cells by NDRG2].

AIM: To explore the induction of HepG2 cells by NDRG2 gene. METHODS: Human NDRG2 gene was obtained by RT-PCR. Sequence analysis proved that sequence of NDRG2 gene was correct. Then the gene was inserted into the eukaryotic expression vector pIRES2-EGFP and transfected into NDRG2 gene-negative HepG2 cells. The changes of cell morphology and structure were observed under light, fluorescence and transmission electron microscope, respectively. The variation of cell cycle was detected by flow cytometry. RESULTS: The NDRG2 gene had been obtained and its expression vector was constructed successfully. The NDRG2 gene-transfected HepG2 cells were shown a bad condition, structure damage, and a great number of died cells. The expression of target gene in cytoplasm of HepG2 cells was seen under fluorescence microscope. Flow cytometry analysis showed G1 phase arrest and apoptosis peak appeared.The typical manifestation of cell apoptosis could be observed under transmission electron microscope. CONCLUSION: NDRG2 gene can arrest HepG2 cell proliferation and induce their apoptosis.

Apoptosis↗
PubMed2002

Effect of coenzyme I on apoptosis of liver cell line L02 induced by ischemia-reperfusion injury.

OBJECTIVE: To study the protective effect of coenzyme I (NADH) on normal liver cell line L02 against ischemia-reperfusion injury. METHODS: Cultured L02 cells was divided into 3 groups, namely ischemia-reperfusion group (I/R), ischemia-reperfusion group with NADH pretreatment (NADH+I/R) and control group. Assisted by flow cytometry (FCM), the apoptosis rate and the expression of apoptosis-related proteins (Bcl-2, p16, p21, p53) were detected in L02 cells in the 3 groups. Apoptotic L02 cells were also observed under transmission electron microscope. RESULTS: I/R significantly inhibited L02 cell proliferation and increased their apoptosis rate up to (31.53+/-8.27)% 12 h after I/R. In the presence of NADH, however, the apoptotic rate of the cells was significantly decreased to (12.61+/-2.34)% (P<0.05). FCM indicated that I/R down-regulated the expression of Bcl-2 and up-regulated the expression of p16, p21 and p53 proteins as compared with control group, but NADH acted to the reverse effect. Typical apoptotic features were observed by transmission electron microscope in the cells 12 h after I/R. CONCLUSION: I/R might induce apoptosis in L02 cells by up-regulating the expression of p16, p21, p53 and down-regulating the expression of Bcl-2, and the protective effect of NADH against I/R-induced apoptosis may lie in the expression regulation of the proteins concerned.

Apoptosis↗
PubMed2002

Palliative operation procedures for pancreatic head carcinoma.

OBJECTIVE: To investigate the procedure choice of palliative operation for carcinoma of the head of the pancreas (CHP). METHODS: The clinical data from 187 patients with CHP treated in the last 20 years were analyzed retrospectively. RESULTS: The operation mortality rate was 8.6%, the mortality of hepatic duct-jejunostomy (HDJS) was not higher than that of cholecystojejunostomy (CJS) (P>0.05). The postoperative relapse of jaundice and cholangitis was significantly lower than that of CJS (P<0.025), while the survival was apparently higher than that of CJS (P<0.01). The mortality of HJDS or CJS with gastrojejunostomy (GJS) was not significantly higher than that of the simple procedure without GJS (P>0.05), whereas the survival was significantly higher than that of the simple procedure without GJS (P<0.01). The occurrence of duodenal obstruction after HDJS or CJS was 29.3%. CONCLUSION: As a palliative operation, Roux-en-Y choledochojejunostomy especially in combination with preventive gastrojejunostomy is strongly recommended.

Adult↗
PubMed2002

Procedures for congenital choledochal cysts and curative effect analysis in adults.

OBJECTIVE: To evaluate the procedures and timing of operation as well as long-term postoperative effect of congenital choledochal cysts (CCC) in adults. METHODS: The procedures and timing of operation, effective rate, re-operation rate and incidence of carcinoma after operation for 70 adult patients with CCC from January 1980 to June 1999 were analyzed retrospectively. RESULTS: The re-operation rate of external drainage was 86% (6/7). The effective rate of internal drainage was significantly lower than that of cyst resection (3/10 vs 45/49, chi2=20.94, P<0.001). The re-operation rate and incidence of carcinoma of internal drainage were higher than those of cyst resection (5/10 vs 3/49, chi2=13.64, P<0.001 and 3/10 vs 3/49, chi2=5.18, P<0.025). The reoperation rate of emergency surgery was higher than that of selective operation (8/10 vs 6/56, chi2=24.37, P<0.001). CONCLUSIONS: External drainage should be the first-aid measure and the therapy of choice on emergency basis. Internal drainage should never be attempted. Cyst resection with Roux-en-Y hepaticojejunostomy is recommended as the treatment of choice in selective operation.

Adenocarcinoma↗