Biomedical subjects

Kelly Johnston

Publications and source records attributed to Kelly Johnston.

4 recordsLinked to original sources

PubMed2006

Implementing an animated geographic information system to investigate factors associated with nosocomial infections: a novel approach.

BACKGROUND: Two million Americans acquire an infection in the hospital each year, resulting in an estimated 88,000 patient deaths per year. OBJECTIVE: Our objective was to describe our initial experience using an animated geographic information system (GIS) to investigate factors associated with nosocomial transmission of resistant organisms. We used a descriptive study at a university-affiliated, county, teaching hospital. We studied all patients and nursing staff on 4 adult, general medicine wards from June through August 2004. RESULTS: We developed and implemented GIS software. GIS-generated animations demonstrated inappropriate patient placement for 19% of patients with methicillin-resistant Staphylococcus aureus and insufficient time for hand hygiene in 14% (6248) of health care provider-patient contacts. CONCLUSION: Animated GIS can uncover previously hidden factors that contribute to the spread of nosocomial infections. This technology may become a useful adjunct for the prevention of nosocomial transmission of infectious agents.

Computer Graphics↗

PubMed2005

Dietary polyphenols decrease glucose uptake by human intestinal Caco-2 cells.

The effect of different classes of dietary polyphenols on intestinal glucose uptake was investigated using polarised Caco-2 intestinal cells. Glucose uptake into cells under sodium-dependent conditions was inhibited by flavonoid glycosides and non-glycosylated polyphenols whereas aglycones and phenolic acids were without effect. Under sodium-free conditions, aglycones and non-glycosylated polyphenols inhibited glucose uptake whereas glycosides and phenolic acids were ineffective. These data suggest that aglycones inhibit facilitated glucose uptake whereas glycosides inhibit the active transport of glucose. The non-glycosylated dietary polyphenols appear to exert their effects via steric hindrance, and (-)-epigallochatechingallate, (-)-epichatechingallate and (-)-epigallochatechin are effective against both transporters.

Biological Transport↗

PubMed2004

Tumour necrosis factor alpha regulates iron transport and transporter expression in human intestinal epithelial cells.

TNFalpha has dramatic effects on iron metabolism contributing to the generation of hypoferraemia in the anaemia of chronic disease. Interestingly, TNFalpha is also synthesised and released within the intestinal mucosa, suggesting that this pro-inflammatory cytokine may play a role in regulating dietary iron absorption. To investigate this possibility, we stimulated intestinal Caco-2 cells with TNFalpha (10 ng/ml). In TNFalpha-treated cells, apical iron uptake was significantly decreased and this was accompanied by a reduction in divalent metal transporter protein and mRNA expression. Our data suggest that TNFalpha could regulate dietary iron absorption and that the apical transport machinery is the target for these actions.

Biological Transport↗

PubMed2003

A survey of anthelmintic resistant nematode parasites in Scottish sheep flocks.

The state of anthelmintic resistance in Scottish sheep flocks was evaluated during April-August 2000 using in vitro bioassays, egg hatch assays for detecting thiabendazole (TBZ) resistance and larval development assays for levamisole, ivermectin and TBZ resistance. Anaerobic sampling kits and detailed questionnaires outlining farm demographics and current management practices were mailed to 227 Moredun Foundation member farms, replies were received from 98 farms. Kits received from 90 farms contained sufficient material to conduct one or more of the assays. A majority of the farms examined (64%) exhibited TBZ resistance but there were both farm locality and regional variations in the percentage of resistant farms. Teladorsagia was the predominant genera detected from farms. No resistance to levamisole or ivermectin was detected in any of the samples. There was no strong evidence from this survey that any of the management practices examined greatly affected TBZ resistance.

Animal Husbandry↗