Biomedical subjects

Kentaro Fujita

Publications and source records attributed to Kentaro Fujita.

6 recordsLinked to original sources

PubMed2006

Host range and receptor utilization of canine distemper virus analyzed by recombinant viruses: Involvement of heparin-like molecule in CDV infection.

We constructed recombinant viruses expressing enhanced green fluorescent protein (EGFP) or firefly luciferase from cDNA clones of the canine distemper virus (CDV) (a Japanese field isolate, Yanaka strain). Using these viruses, we examined susceptibilities of different cell lines to CDV infection. The results revealed that the recombinant CDVs can infect a broad range of cell lines. Infectivity inhibition assay using a monoclonal antibody specific to the human SLAM molecule indicated that the infection of B95a cells with these recombinant CDVs is mainly mediated by SLAM but the infection of 293 cell lines with CDV is not, implying the presence of one or more alternative receptors for CDV in non-lymphoid tissue. Infection of 293 cells with the recombinant CDV was inhibited by soluble heparin, and the recombinant virus bound to immobilized heparin. Both F and H proteins of CDV could bind to immobilized heparin. These results suggest that heparin-like molecules are involved in CDV infection.

Animals↗

PubMed2006

Sequence analysis of VP2 gene of canine parvovirus isolated from domestic dogs in Japan in 1999 and 2000.

Seven strains of canine parvovirus (CPV) were isolated from affected dogs in Japan between 1999 and 2000, and their VP2 genes were genetically analyzed. Comparison of the predicted amino acid sequences of VP2 suggested that three field isolates corresponded to CPV type 2a, while the other four to CPV type 2b. The phylogenetic tree constructed from the VP2 genes showed that the newly isolated strains are classified into the cluster consisting of recent Japanese and Taiwanese field isolates, which are distinct from Vietnamese isolates, United States Isolates, or classical CPV type 2. These results suggest that the CPV transmission occurred between Japan and Taiwan in 1990s, and the offspring are still circulating in both countries.

Amino Acid Sequence↗

PubMed2006

Construal levels and self-control.

The authors propose that self-control involves making decisions and behaving in a manner consistent with high-level versus low-level construals of a situation. Activation of high-level construals (which capture global, superordinate, primary features of an event) should lead to greater self-control than activation of low-level construals (which capture local, subordinate, secondary features). In 6 experiments using 3 different techniques, the authors manipulated construal levels and assessed their effects on self-control and underlying psychological processes. High-level construals led to decreased preferences for immediate over delayed outcomes, greater physical endurance, stronger intentions to exert self-control, and less positive evaluations of temptations that undermine self-control. These results support a construal-level analysis of self-control.

Affect↗

PubMed2006

Transcending the "here": the effect of spatial distance on social judgment.

Construal level theory proposes that increasing the reported spatial distance of events produces judgments that reflect abstract, schematic representations of the events. Across 4 experiments, the authors examined the impact of spatial distance on construal-dependent social judgments. Participants structured behavior into fewer, broader units (Study 1) and increasingly attributed behavior to enduring dispositions rather than situational constraints (Study 2) when the behavior was spatially distant rather than near. Participants reported that typical events were more likely and atypical events less likely when events were more spatially distant (Study 3). They were also less likely to extrapolate from specific cases that deviated from general trends when making predictions about more spatially distant events (Study 4). Implications for social judgment are discussed.

Analysis of Variance↗

PubMed2006

Spatial distance and mental construal of social events.

Construal-level theory proposes that increasing the reported spatial distance of events leads individuals to represent the events by their central, abstract, global features (high-level construal) rather than by their peripheral, concrete, local features (low-level construal). Results of two experiments indicated that participants preferred to identify actions as ends rather than as means to a greater extent when these actions occurred at a spatially distant, as opposed to near, location (Study 1), and that they used more abstract language to recall spatially distant events, compared with near events (Study 2). These findings suggest that spatially distant events are associated with high-level construals, and that spatial distance can be conceptualized as a dimension of psychological distance.

Cognition↗

PubMed2002

Stereotype performance boosts: the impact of self-relevance and the manner of stereotype activation.

The activation of positive stereotypes has been shown to produce academic performance boosts. Evidence regarding the role of self-relevance in producing such effects has been mixed. The authors propose that the subtlety of stereotype activation plays a key role in creating performance boosts among targets and nontargets of stereotypes. Study 1 found that subtle stereotype activation boosted performance in targets, but blatant activation did not. Study 2 was conducted on both targets and nontargets using different methods of stereotype activation. Again, targets showed performance boosts when stereotypes were subtly activated but not when they were blatantly activated. Nontargets, however, showed boosts in performance only when stereotypes were blatantly activated. The role of self-relevance in mediating sensitivity to stimuli is discussed.

Achievement↗