Biomedical subjects

Koichi Kaneko

Publications and source records attributed to Koichi Kaneko.

4 recordsLinked to original sources

PubMed2006

Mutations in the SLC34A2 gene are associated with pulmonary alveolar microlithiasis.

RATIONALE: Pulmonary alveolar microlithiasis is an autosomal recessive disorder in which microliths are formed in the alveolar space. OBJECTIVES: To identify the responsible gene that causes pulmonary alveolar microlithiasis. METHODS: By means of a genomewide single-nucleotide polymorphism analysis using DNA from three patients, we have narrowed the region in which the candidate gene is located. From this region, we have identified a gene that has mutations in all patients with pulmonary alveolar microlithiasis. MEASUREMENTS AND MAIN RESULTS: We identified a candidate gene, SLC34A2, that encodes a type IIb sodium phosphate cotransporter and that is mutated in six of six patients investigated. SLC34A2 is specifically expressed in type II alveolar cells, and the mutations abolished the normal gene function. CONCLUSION: Mutations in the SLC34A2 gene that abolish normal gene function cause pulmonary alveolar microlithiasis.

Amino Acid Sequence↗

PubMed2006

[Minimally invasive thoracotomy for the lung resection; without thoracoscopic procedure].

Video-assisted thoracoscopic surgery (VATS) is though to be a minimally invasive surgical procedure of lung resection. But there are some problems still remained about training, cost efficacy and oncological principles. Minimally invasive thoracotomy without thoracoscopic procedure was reviewed in this paper. In case of standard posterolateral thoracotomy with cutting ribs, it brings less postoperative pain and less dislocation of the ribs that fixes the ribs with bioabsorbable poly-L-lactide (PLLA) pins. Muscle sparing thoracotomy is available in broadly peeling off the muscle of trapezius and latissimus dorsi subcutaneously. But sometime much exudate collects subcutaneous wound in postoperative period so that some trouble or complication often occurs in the management of postoperative course. In case of axilloanterior thoracotomy we have proper view which is almost the same as standard posterolateral thoracotomy without cutting thoracic muscles. Skin incision runs from the axillar to the lateral margin of the breast and the 4th or 5th intercostal thoracotomy is performed through thoracodorsal muscle being pressed laterally, anterior serratus muscle being split. There is no need to cut the ribs, and good for the upper or middle lobectomy and the upper mediastinal dissection. Axilloanterior thoracotomy is minimally invasive than standard posterolateral thoracotomy and is useful for the surgery of upper or middle lobe lung cancer. In the procedure of thoracotomy operating with or without thoracoscope is not to be concerned. Important is to perform operation smoothly in adequate time for patients ease.

Humans↗

PubMed2004

[Pneumothorax].

Most of the patients with spontaneous pneumothorax are young male adults without significant pulmonary disease and the prognosis in these patients is usually very good. But in a few cases when tension pneumothorax develops, immediate diagnosis and chest tube drainage is required to avoid life-threatening emergency. Needless to say mechanical ventilation with tracheal intubation is contraindication even if the patient has severe respiratory distress or hypoxia. Re-expansion pulmonary edema is another severe complication. This occurs to a long-term (over 1 week) complete collapse lung when it is rapidly re-expanded with high negative pressure. To treat such patient it is necessary to decompress the lung slowly using lower negative pressure or water seal suction. When persistent bleeding of hemothorax is present, it is important to decide to perform open thoracotomy or video-assisted thoracoscopic surgery to avoid blood transfusion. The diagnosis of pneumothorax is simply done by chest X-ray film. When the pneumothorax is suspected, chest X-ray examination has to be done first. A scheduled operation to prevent recurrence of pneumothorax has to be performed under relative indication in consideration of both the medical factor and the social factor of the patient.

Adult↗

PubMed2003

[Impact of cimetidine on growth and metastasis of surgically transplanted Lewis lung cancer].

PURPOSE: Histamine has multiple biological functions and its immunomodulatory actions are not fully understood. In the current study, we investigated the effect of cimetidine, which is a histamine-2 receptor antagonist, on the progress of transplanted Lewis lung cancers using a mouse model. METHODS: A piece of Lewis lung cancer weighing 15 mg was transplanted into the subcutaneous space on the back of each C 57 BL/6 mouse. Mice were randomized into a no-treatment control group (CO) or one of two treatment groups. In the treatment groups, 16 mg/kg/day (LD) or 160 mg/kg/day (HD) of cimetidine was orally administered from one week before the day of transplantation to the time of sacrifice. Subcutaneous tumors and lungs were excised on the 28th or 42nd postoperative day. RESULTS: The mean vascular densities of the subcutaneous tumors on day 28 were 55.7 +/- 23.9/mm2 in CO, 88.0 +/- 16.3/mm2 in LD and 122.6 +/- 16.9/mm2 in HD (p < 0.05; CO vs. LD or HD, LD vs. HD). On day 42, mean weights of the subcutaneous tumors and the numbers of metastatic lung tumors were 6.0 +/- 2.1 g in CO, 7.9 +/- 1.2 g in LD and 10.0 +/- 1.9 g in HD (p < 0.05; CO vs. HD), and 7.5 +/- 6.0 in CO, 17.0 +/- 3.0 in LD and 19.8 +/- 7.4 in HD (p < 0.05; CO vs. HD), respectively. CONCLUSIONS: These results suggest that cimetidine dose-dependently enhances the angiogenesis, growth and metastasis of surgically transplanted Lewis lung cancer in a mouse model of this type.

Animals↗