Biomedical subjects

Krystyna Stec-Michalska

Publications and source records attributed to Krystyna Stec-Michalska.

6 recordsLinked to original sources

PubMed2006

[Nocturnal secretion of melatonin in subjects with asymptomatic and symptomatic Helicobacter pylori infection].

UNLABELLED: Melatonin is synthesized not only in the pineal gland, but also in gastrointestinal tract by enterochromaffin cells (EC) and it has a gastroprotective properties. Helicobacter pylori infection causes functional and organic changes of gastric mucosa, particularly in endocrine cells (D, G, ECL, EC). AIM: The aim of own studies was to evaluate nocturnal secretion of Melatonin in subjects with asymptomatic and symptomatic (dyspepsia, duodenal ulcer disease) H. pylori infection (Hp). MATERIAL AND METHODS: 116 subjects, aged 19-61 years were included in this study. Four groups were distinguished: group I (n=30) healthy subjects, Hp (-), group II (n=26)--subjects with asymptomatic infection Hp, group III (n=32) patients with ulcer-like dyspepsia, Hp (+), and group IV (n=28) patients with duodenal ulcer Hp(+). H. pylori infection was diagnosed by using urea breath test (UBT-(13)C) and rapid urease test. The concentration of melatonin in serum was measured with ELISA method, before and after eradication of H. pylori. Blood samples were taken for examination at 10:00 p.m., 2:00 a.m. and 6:00 a.m. RESULTS: The average melatonin concentration at night hours was found in group I--34.74 +/- 4.77 pg/ml, in group II--48.29 +/- 12.22 pg/ml (p < O.005), in group III--41.56 +/- 12.31 pg/ml (p > 0.05) and in group IV--26.16 +/- 6.89 pg/ml p < 0.01). Such statistical differences were not observed in all group after eradication H. pylori. CONCLUSIONS: (1) Nocturnal secretion of melatonin in subjects with asymptomatic infection of H. pylori is higher than in patients with ulcer-like dyspepsia and with duodenal ulcer disease. (2) Lower nocturnal secretion of melatonin probably may play role in pathogenesis of upper digestive tract diseases.

Adult↗

PubMed2005

Loss of FHIT expression in gastric mucosa of patients with family histories of gastric cancer and Helicobacter pylori infection.

AIM: To answer the question whether FHIT gene expression is affected by the family history of gastric carcinoma and the presence of Helicobacter pylori (H pylori) in the gastric mucosa of patients with dyspepsia. METHODS: FHIT gene expression in two different topographic sites of the gastric mucosa of twenty-one patients with dyspepsia and with or without familial gastric carcinoma, infected or not infected with H pylori, was evaluated by reverse transcription-PCR (RT-PCR) and IMAGE QUANT methods. A rapid urease test and histopathological examination were used to determine H pylori colonization. RESULTS: In the gastric mucosa of patients with family histories of gastric carcinoma, the amount of FHIT protein mRNA was reduced down to 32%, and for patients with H pylori colonization, to 24% in comparison to controls with dyspepsia and without cancer in the family. FHIT expression was independent of the topography of specimens (corpus vs antrum), and for the control patients it was less sensitive to infection with H pylori. A considerable statistical difference in FHIT levels was observed in the gastric mucosa from the corpus of patients with family histories of gastric carcinoma in respect to H pylori colonization (P = 0.06). Macroscopic evaluation of the gastric mucosa demonstrated that pathologic changes classified according to the Sydney system had no significant influence on FHIT expression within each tested group of patients. CONCLUSION: Loss of FHIT expression was observed in patients with dyspepsia and family histories of gastric carcinoma, especially those infected with H pylori. Such results may constitute an early indication of the development of gastric carcinoma, which is associated with family factors including heredity and H pylori infection. The loss of the FHIT gene may serve as a marker for early diagnosis and prevention of gastric carcinoma, especially in context of early monitoring of H pylori infection in individuals with a record of familial stomach cancer.

Acid Anhydride Hydrolases↗

PubMed2004

[Inflammatory bowel diseases--imaging diagnostics].

The basic diagnostic procedure in ulcerative colitis is an endoscopy of gastrointestinal tract. It allows the macroscopic evaluation as well as the specimen taking for histological assessment what is the basis for ultimate diagnosis. In case of Crohn's disease the radiological diagnostics is of equal importance as endoscope evaluation. The imaging of inflammatory changes in Crohn's disease still poses some difficulties, especially, that located in the small intestine. Lately, the range of accessible examinations has been wider. We have in disposal the ultrasonography, the computed tomography, the magnetic resonance imaging and the capsular endoscopy. All of them are of great use in the diagnosis of Crohn's disease. In case of microscopic colitis all the imaging diagnostics has no use. The only one mean to establish the diagnosis is a histological assessment. What is more, in the period of remission the colon tissue could be normal. In this paper we discussed the traditional and contemporary intestine imaging methods in inflammatory bowel diseases. The conclusion is that the further progress in science offers a better imaging and, what is even more important, the more efficient diagnostics and treatment of these diseases.

Humans↗

PubMed2004

[Early diagnosis of colorectal cancer].

Colorectal cancer is the fourth most common cancer in the world and the second most common and most frequently mentioned cause of cancer death in Poland. Death from colorectal cancer can be reduced through early diagnosis. Currently there are several screening methods: faecal occult blood tests, sigmoidoscopy, colonoscopy, barium enema and emerging methods like virtual colonoscopy and faecal DNA testing. In this article we show the efficacy of these tests with respect to their sensitivity, specificity, costs and safety. Patients at high-risk of colorectal cancer were also considered. There is an urgent need to standardize the methods of diagnosis in patients at high-risk to increasing accessibility and quality of tests.

Biopsy↗

PubMed2004

[Estimation of gastric mucosa morphological changes in subjects with asymptomatic Helicobacter pylori infection and family history of gastric cancer].

Helicobacter pylori is a known carcinogen but the natural history of gastric carcinoma development has not been recognized thoroughly and early diagnostics is difficult particularly in asymptomatic infection. The aim of own study was gastric mucosa morphological estimation in subjects with asymptomatic H. pylori infection. Investigations were performed in 60 subjects, aged 24-55 years with family history of gastric cancer and with asymptomatic (group I; n=30) and symptomatic infection (group II; n=30). The infection and its severity were confirmed with breath test with the use of 75 mg of 13C labelled urea and Olympus FANci-2 analyzer. Gastric mucosa macro- and microscopic state was evaluated according to 4-degree Sydney scale. In group II macroscopic changes were more pronounced than in group I (p=0.032); no such differences were seen in microscopic picture (p=0.625). The extent of macro- and microscopic changes demonstrated positive correlation with the results of breath test (group I: r=0.8169; group II: r=0.8393). In subjects with family history of gastric cancer H. pylori infection both, symptomatic and asymptomatic causes similar gastric mucosa histopathological changes including intestinal metaplasia. The degree of morphological changes demonstrates positive dependence with the severity of infection.

Adult↗

PubMed2004

[Assessment of Helicobacter pylori infection severity and immune response in different age groups].

In practice, breath and serologic tests are applied to confirm the diagnosis of H. pylori and for epidemiologic studies. The aim of the study is quantitative evaluation of the results of both tests in relation to the examined subjects' age, clinical symptoms and family history of gastric cancer. Investigations were carried out in 300 subjects, aged 16-70 years, 186 of them with asymptomatic H. pylori infection and 114 with family history of gastric cancer. The breath test was performed with 13C-labelled urea and Olympus FANci-2 analyzer and antibody titer was determined with ELISA in serologic test. The infection severity was the highest in the age group 31-40 years (result of the breath test 32.4+/-20.3/1000) and the lowest in the group aged 61-70 years (8.4+/-8.11/1000; p<0.01). The antibody titer was the highest at the age 21-30 years (89.6+/-39.5 U/ml) and significantly lower in the group over 60 years of age (31.0+/-27.2 U/ml; p<0.01). In subjects with asymptomatic infection the results of both tests were lower than in symptomatic infection in all age groups. No statistically significant differences were found between the subjects with and without family history of gastric cancer. H. pylori infection severity and immune response depend on the age of the examined subjects. H. pylori infection severity and immune response are lower in asymptomatic than in symptomatic subjects.

Adolescent↗