Biomedical subjects

Kunling Zhang

Publications and source records attributed to Kunling Zhang.

3 recordsLinked to original sources

PubMed2004

[Using PCR method to study the EB virus DNA in NPC].

OBJECTIVE: To study the EB virus DNA distribution in paraffin-embedded tissue, peripheral blood leukocyte and serum in nasopharyngeal carcinoma patients (NPC). METHOD: PCR method, in paraffin-embedded tissue of 39 patients with NPC (NPC group-1) and 20 patients with chronic nasopharyngitis (control group-1), in peripheral blood leukocyte and serum of 24 patients with NPC (NPC group-2) and 10 patients with other head and neck carcinoma (control group-2) and 10 patients with chronic nasopharyngitis (control group-3) were used to test the presence of Epstein-Barr virus DNA. At the same time, we also measured serum EB virus VCA-IgA antibodies with enzyme-immunoassay in NPC group-2, control group-2 and control group-3. RESULT: The results showed that 28 of 39 nasopharyngeal carcinoma patients (71.8%) (NPC group-1) and 3 of 20 chronic nasopharyngitis (15%) (control group-1) were EB virus DNA positive in paraffin-embedded tissues. The difference between NPC group-1 and control group-1 was statistically significant. Ten of 24 NPC group-2 (41.67%) and 1 of 10 control group-2 (10%) were EB virus DNA positive in peripheral blood leukocyte. Control group-3 EB virus DNA was negative in peripheral blood leukocyte. The difference between NPC group-2 and control group-3 was statistically significant (P < 0.05). All of the patients' EB virus DNA were negative in serum. The difference between NPC group-2 and control group-2; 2 was statistically significant in VCA-IgA titer respectively (Ridit Test P < 0.01). CONCLUSION: EB virus DNA positive in paraffin-embedded tissues is higher than that in peripheral blood leukocyte in nasopharyngeal carcinoma patients. In serum EB virus DNA is negative. The results suggest that EB virus DNA positive from paraffin-embedded tissues to peripheral blood leukocyte to serum is decrease gradually. There must be some relationship between VCA-IgA in serum and EB virus DNA in peripheral blood leukocyte.

Adolescent↗

PubMed2003

[A clinical study on improving phonation function of larynx after partial laryngectomy].

OBJECTIVE: To analyse the clinical results of reconstruction of vocal cord using sternohyoid muscle flap after partial laryngectomy and research a new repairing method to improve laryngeal function and living quality of patient. METHOD: The pronunciation of larynx is on the basic of the vibration of the vocal cord by air current. The laryngeal appearance and the function of opposite side vocal cord is very important for improving the quality of pronunciation. We developed a technique of thyroid cartilage membrane and sternohyoid muscle flap to repair the defect left by a vertical laryngectomy and reconstructed the vocal cord. The thyroid cartilage membrane was used to bridge over the upper part defect of laryngeal cavity, the sternohyoid muscle flap forming the opposite side was made a 90 degree bent toward the laryngeal cavity to repaired the lower part defect of laryngeal cavity and reconstruct a new vocal cord. The repaired larynx recovered the normal appearance. The new vocal cord participated speech. The anterior 2/3 part of both vocal cored could contacted in pronunciation of patient. The phonation function of larynx was restored. RESULT: Sixty-one cases partial laryngectomy was taken including vertical partial laryngectomy in 37 cases, extended vertical partial laryngectomy in 24. During their operations, the laryngeal defect was repaired with thyroid cartilage membrane and sternohyoid muscle flap, and a vocal cord was reconstructed in the same time. After operation, the laryngeal cavity repaired has a normal appearance, and the laryngeal satisfactory phonation effect has been obtained. 93.4% of the patients enjoyed a socially acceptable voice and rejoined normal working. CONCLUSION: A technique of thyroid cartilage and sternohyoid muscle flap to repair the defect and reconstructive vocal cord after partial laryngectomy make patient recover normal laryngeal appearance and obtain satisfactory phonation effect. Among the method of repairation for partial laryngectomy sternohyoid muscle flap should be first considered owing to its various merits: 1. abundant material. 2. convenient procedures. 3. smaller trauma. 4. satisfactory effect.

Adult↗

PubMed2003

[Pearson technique in the treatment of advanced laryngeal cancer].

OBJECTIVE: To seek for a better method of treating the advanced laryngeal cancer. METHOD: Twenty-eight cases of advanced laryngeal cancer were treated with near-total laryngectomy(Pearson technique), including 11 glottic cancers(III stage, 8 cases: IV stage, 3 cases), 17 supraglottic cancers (III stage, 13 cases; IV stage, 4 cases). In the same time 26 patients had neck node dissections. All the cases had speech function reconstructions. RESULT: The speech function was obtained in 27/28(96.4%). All cases had good swallowing, 3-year survival rate is 78.6%, 5-year survival rate is 68.4%. CONCLUSION: Near-total laryngectomy can be used in most cases that might be treated with traditional total laryngectomy in the past. It can provide satisfied survival rate, good swallowing, better speech function, but breath function.

Aged↗