Biomedical subjects

Kyoko Tabuchi

Publications and source records attributed to Kyoko Tabuchi.

3 recordsLinked to original sources

PubMed2004

[The relationship between Staphylococcus aureus and atopic keratoconjunctivitis].

PURPOSE: To investigate the role of Staphylococcus aureus in patients with vernal keratoconjunctivitis (VKC) and atopic keratoconjunctivitis (AKC) complicated by atopic dermatitis (AD). SUBJECTS AND METHODS: Microbiological culture from the conjunctival sac of acute exacerbated patients with VKC and AKC complicated by AD was performed. The subjects were 29 patients (31 eyes) with VKC and AKC who showed acute exacerbated clinical symptoms of conjunctivitis. Antigen specific IgE antibodies to staphylococcal enterotoxin A (SEA) and staphylococcal enterotoxin B (SEB) in serum were also examined. In this study, the patients were divided into two groups: An AD group consisting of 22 patients with VKC and AKC complicated by AD, and a control group consisting of 8 patients with allergic conjunctivitis without AD. We also examined SEA/SEB specific IgE antibody in tears from the 5 patients who underwent bacteriological examinations of the conjunctival sac at the same time. RESULTS: Twenty-seven out of 31 eyes were gram-positive in the bacteriological culture from the conjunctival sac (87.1%). Staphylococcus aureus was detected in 21 out of the 27 eyes (77.8%). In the AD group, 2 of the 22 cases were gram-positive for serum SEA specific IgE antibodies, 2 cases of the 22 cases were gram-positive for SEB specific IgE antibodies, and 9 cases were gram-positive for both SEA and SEB specific IgE antibodies. Serum SEA and SEB specific IgE antibodies were all gram-negative in the control group. Either SEA or SEB specific IgE antibody in tears was detected in all of the above 5 patients who underwent bacteriological examinations of the conjunctival sac, and 4 of the 5 were gram-positive. Staphylococcus aureus was isolated in 3 out of the 5 patients, and 1 case was gram-negative. CONCLUSION: Staphylococcus is one of the exacerbating factors in VKC and AKC. It is important to evaluate both bacteriological examinations of the conjunctival sac and SEA/SEB specific IgE in tears.

Conjunctiva↗

PubMed2002

[Clinical study on surgical outcome of penetrating keratoplasty for herpetic leukoma].

PURPOSE: To evaluate prognostic factors, rejection, and recurrence of herpetic keratitis, in keratoplasty for herpetic leukoma. SUBJECTS AND METHODS: We compared surgical outcome between patients who underwent keratoplasty for herpetic leukoma and those who received it for non-herpetic disorders. We compared the eyes of 24 patients in the herpetic group with 17 eyes of 20 patients with non-herpetic corneal disorders, i.e., the non-herpetic group. RESULTS: The rejection rate was 45.8% for the herpetic group and 5.00% for the non-herpetic group, showing significant difference(p < 0.05). Transparent grafts were obtained for 75.0% of the herpetic group and 85.0% of the non-herpetic group, giving no significant difference. In the herpetic group, graft transparency in eyes with recurrences were 94.1%(p < 0.05). Graft rejection occurred mostly within 1 year postoperatively. RecuRrent herpetic keratitis tended to occur within 3 years and it was later than graft rejection. CONCLUSION: In patients who underwent keratoplasty for herpetic leukoma, steroid therapy is important for 1 postoperative year to suppress graft rejection, but after that, steroid treatment should be carefully considered to minimize the risk of recurrences of herpetic keratitis.

Aged↗

PubMedDate not supplied

Histological study on mast cells in conjunctiva of NC/Nga mice.

PURPOSE: To investigate the pathological findings in conjunctiva of NC/Nga mice, which develop atopic dermatitis spontaneously, we focused our study on the density of mast cells as determined by histological methods. METHODS: NC/Nga mice were divided into five groups; the 4W-group comprised four 4-week-old mice without treatment, the 10W-group comprised four 10-week-old mice without treatment, the 16W-group comprised four 16-week-old mice without treatment, the Dx group comprised three 16-week-old mice undergoing topical 0.1% dexamethasone (Dx) ointment treatment, and the FK506 group comprised three 16-week-old mice undergoing topical 0.1% FK506 ointment treatment. For the histological examination, the lids and eyeballs of the mice were removed and fixed with Carnoy's solution and thin sections were made. The Carnoy-fixed specimens were stained with toluidine blue or H&E and examined histologically. Toluidine blue-stained tissue sections were examined for the density per square millimeter of mast cells, which were identified as metachromatic cells in the conjunctival tissue and lid cutaneous tissue by light microscopy. RESULTS: Mast cell density in the conjunctiva was 8.6 +/- 8.2 cells/mm(2) in the 4W-group, 29.2 +/- 22.0 cells/mm(2) in the 10W-group, 41.0 +/- 21.1 cells/mm(2) in the 16W-group, 22.7 +/- 17.1 cells/mm(2) in the Dx group, and 33.6 +/- 27.7 cells/mm(2) in the FK506 group. Mast cell density increased significantly with age among the 4W-, 10W-, and 16W-groups ( P < 0.001). The mast cell density of lid cutaneous tissue was higher than the conjunctival mast cell density. Mast cell density was significantly higher in the 16W-group than in the Dx group, but not significantly higher than in the FK506 group. CONCLUSIONS: An increase in mast cell density with age indicates that NC/Nga mice develop atopic keratoconjunctivitis-like signs. Dexamethasone ointment has a suppressive effect on the increase in mast cell density.

Aging↗