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Biomedical subjects

L A Belova

Publications and source records attributed to L A Belova.

At least 19 recordsLinked to original sources
PubMed2003

[Comparison of the trypsin inhibitor activity in urine of donors and patients with kidney diseases].

The general and acid-stable activities, i.e. anti-tryptic activity (ATA) and anti-chemotryptic activity (ACTA), of a trypsin inhibiter in the urine (TIU) were determined for patients and donors. A lack of reliable differences between the general and acid-stable activities in donors and in patients with nephritis is indicative of that TIU is the main inhibiter of trypsin in human urine. A correlation degree between the general ATA and ACTA (r > 0.7) means that the two-domain inhibiter form is excreted into the urine. A comparison between the general and acid-stable ATAs suggests the presence of other trypsin inhibiters in the urine of patients.

Humans↗
PubMed2003

[Imbalance in the proteinase-inhibitor system in obstetric sepsis and septic shock].

The condition of proteinase-inhibitor balance was studied in blood plasma of patients with obstetric sepsis. Hypoproteinemia (due to a massive blood loss and toxic effect) was shown to result in a 2-5-fold decrease of the activity of endogenous proteinases in blood plasma versus the control group. The activity of alpha-macroglobulins was also by 2 times lower versus the controls, while the activity of alpha 1-antichymotrypsin and of an acid-stable inhibitor of trypsin was found to be higher by 20-30%. A degree of imbalance of proteinase/inhibitor depends on a disease severity.

Endopeptidases↗
PubMed2003

[Daily blood pressure profile in patients with hypertensive encephalopathy].

The purpose of the study was to study daily variations of blood pressure (BP) in patients with hypertensive encephalopathy (HE). Daily BP monitoring (DBPM) was made in 100 patients with different stages of HE. In HE, a proportion of patients from a non-dippers group substantially increased. An excessive nocturnal reduction in systolic BP (SBP) was more common. A dippers group was predominant in the controls. The daily index for SBP significantly decreased in patients with stage I HE; that for diastolic BP (DBP) reduced in those with stages I-II HE. The morning increase rate (MIC) of BP was studied. MIC of SBP was higher in patients with HE, which was most commonly observed in the non-dippers group. There was a more significant reduction in DBP in combination with high MIC in 10% of the examinees.

Adult↗
PubMed2000

Angiotensin II-generating enzymes.

The renin--angiotensin system (RAS) is a system of enzymes and hormones that regulate blood pressure and electrolyte and fluid homeostasis in mammals. Angiotensin II (Ang-II) is one of the most important and well-known components of RAS. It is formed from the protein precursor angiotensinogen by the sequential actions of proteolytic enzymes. The classic pathway of Ang-II generation includes a reaction catalyzed by angiotensin-converting enzyme (ACE). However, there are alternative pathways for the generation of Ang-II. In this paper, possible routes of formation of Ang-II in the human body are reviewed. Various Ang-II-generating enzymes (tonin, cathepsin G, chymase, etc.) and their properties are considered. The classification of these enzymes is also considered.

Angiotensin II↗
PubMed2000

[Activity of chymotrypsin-like proteinases in patients with ischemic heart disease, arterial hypertension and nonspecific aortic arteritis].

AIM: To analyze activity of chymotrypsin-like plasma proteinases (CTP) in patients with various cardiovascular diseases. MATERIAL AND METHODS: CTP activity was studied in 82 patients with various cardiovascular diseases: 13 coronary heart disease patients with normal arterial pressure, 49 patients with essential hypertension (EH) and secondary arterial hypertension, 20 patients with nonspecific aortic arteritis. 28 donors served control. RESULTS: CTP activity in plasma of patients with EH rose 4 times compared to donors. If EH patients had concurrent diseases (CHD, chronic pyelonephritis, atherosclerosis of extracranial arteries), CTP activity may increase by 30-300%. In patients with nonspecific aortic arteritis CTP activity in blood plasma is 17.5 times higher than in donors. CONCLUSION: High CTP activity in cardiovascular patients may be explained by chymase and cathepsin G release into blood flow indicating activation of alternative to ACE pathway of angiotensin II production or the presence of the inflammatory process.

Adolescent↗
PubMed1998

[Extracorporeal circulation methods in the treatment of nonspecific aortic arteritis].

AIM: Development of a protocol of multiple-modality treatment of patients with nonspecific aortoarteritis (NAA) making use of plasmapheresis and specific plasma adsorption of proteinases. MATERIALS AND METHODS: Six patients with NAA aged 15 to 58 years were examined using ultrasonic dopplerography, magnetic imaging, and angiography of the large vessels. Red cell sedimentation rate was assessed, cathepsin G activity, antitryptic activity, and content of C-reactive protein in the blood measured. Drug therapy was supplemented by repeated sessions of plasmapheresis and specific plasma adsorption on immotin. RESULTS: After sessions of plasmapheresis (n = 17) and plasma adsorption (n = 13), increased cathepsin G activity dropped by at least 30% (in 3 patients it normalized), the content of C-reactive protein decreased in the presence of normal antitryptic activity (in patients with decreased activity it normalized and in those with increased values a tendency to normalization was observed). Red cell sedimentation rate decreased, particularly so 1 and 2 months after treatment; the patients felt better. CONCLUSION: Multiple-modality treatment of NAA patients making use of plasmapheresis and plasma adsorption decreases the activity of the inflammatory process.

Adolescent↗
PubMed1997

Biochemistry of inflammatory processes and vascular injury. Role of neutrophils: a review.

Possible mechanisms of the participation of neutrophils in inflammation and vascular damage are reviewed. The role of chemotaxis, adhesion, and neutrophil-endothelium interaction are considered. Specific attention is paid to the action of neutrophils (PMNs) in inflammation sites which can be caused by the interaction between PMN-generated proteinases and oxygen metabolites.

Animals↗
PubMed1996

[The biochemical and clinical aspects of the participation of granulocytes and their proteinases in a vascular wall lesion].

Some components of plasma and granulocyte proteolytic system, granulocyte activation degree were evaluated in 15 patients with nonspecific aortic arteritis (NAA) and 12 hereditary hypercholesterolemia (HHC) patients. Catepsin G activity in blood plasma of NAA and HHC patients was higher than in donors 8-9 and 6-fold, respectively (p < 0.001). Granulocyte collagenase activity, spontaneous NBT test values in them were higher than in donors 6 and 4-fold (p < 0.02 and p < 0.01, respectively), 2-fold (p < 0.005 and p < 0.001, respectively). The data obtained confirm the suggestion on the presence of an inflammatory component not only in pathogenesis of NAA, but also in HHC causation.

Adolescent↗
PubMed1994

[Intensification of the effect of exogenous plasminogen activators on lysis of fibrin blood clots due to a decrease in the level of alpha-2- antiplasmin by a plasmasorption method].

Using plasminogen-Sepharose 4B chromatography, a 50-70% reduction in the alpha-2-antiplasmin (alpha-2-AP) content in human and dog blood plasma was reached. The decrease in the alpha-2-AP concentration in human and dog blood plasma markedly enhanced the lysis of fibrin clots formed from the plasma under the action of urokinase and streptokinase. Thus, the lysis of model clots for a definite period of time for human and dog blood plasma depleted for alpha-2-AP by 50-70% required 5-8 and 3-6 times as low urokinase and streptokinase concentrations as those needed for the clot lysis in the native plasma. The decrease in the fibrinogen concentration in human blood plasma caused by plasmosorption on plasminogen-Sepharose enhanced the model fibrin clot lysis by urokinase and streptokinase, however, by no more than 5-10%. Hence, the increased efficiency of plasminogen activators in the given model system can be accounted for by the decline of the antiplasmin potential of the blood plasma.

Animals↗
PubMed1994

[Monoclonal antibodies to the trypsin-binding domain of trypsin inhibitor from human urine].

Three MAb M2, B6, P1 (IgG1 type) against human urinary trypsin inhibitor (UTI), a glycoprotein with antiinflammatory properties, have been produced by hybridization of mouse myeloma cells P3O1 with spleen cells of immunized mice BALB/c. Competitive ELISA-examination of the peroxidase conjugates of M2, B6, and P1 MAb in the presence of the trypsin binding domain shows the M2 antibody to possess the highest affinity for this domain. On the basis of the MAb M2, a competitive ELISA of UTI concentration in urine is proposed. ELISA-detectable changes in the UTI content of urine from patients with nephritis without renal failure can be considered as an early index of renal parenchyma damage.

Animals↗
PubMed1993

[A new method of isolating acid-stable trypsin inhibitor from human urine].

A novel procedure for isolation of an acid-resistant trypsin inhibitor from human urine (UTI) is described. The method is based on the use of immobilized on an insoluble matrix monoclonal antibodies specific for the given inhibitor. The use of Immotin (which represents an immobilized on Sepharose 4B UTI) as precolumn during affinity chromatography makes it possible to significantly prolong the immunoadsorbent life and to increase the inhibitor yield up to 92% of its original content in the urine. The preparation obtained with the use of an immunoadsorbent contains predominantly the native form of the inhibitor whose molecular mass is about 44 kDa.

Antibodies, Monoclonal↗
PubMed1991

[Specific plasma sorption of proteinases--a new approach to the treatment of acute pancreatitis].

The authors treated destructive forms of acute pancreatitis for the first time by means of specific plasma sorption of proteinases with the use of a proteinase plasma sorbent which is an acid-stable proteinase inhibitor immobilized on sepharose (ASI-Sepharose). Specific plasma sorption of proteinases on ASI-Sepharose was applied to the treatment of 29 patients with various forms of acute destructive pancreatitis. At the end of specific plasma sorption procedure the activity of blood plasma proteolytic enzymes in the patients reduced by 60-75%, which was attended by marked improvement of the condition in most cases; aggravation of the patients' condition was not encountered. The mortality in this group was 20.7% and was due to complications occurring during the development of the main disease. The mortality rate among patients treated by specific proteinase plasma sorption is much lower than that recorded in the USSR for destructive forms of acute pancreatitis which ranges from 30 to 75%.

Acute Disease↗
PubMed1990

[Specific proteinase plasma sorption in surgery with artificial blood circulation].

Cardiosurgery with artificial circulation (AC) is attended with activation of plasma cascade proteolytic systems, which leads to homeostatic disturbances and postoperative complications. AC with the use of specific proteinase plasma sorption in a column with sepharose-immobilized acid-base proteinase inhibitor from human urine prevents activation of proteinase in the plasma. Application of specific plasma sorption facilitates rapid restoration of the plasma protein concentration lowered during AC, improves the course of the postoperative period, and reduces the risk of pulmonary, cardiovascular, and nervous complications.

Adult↗
PubMed1988

Effect of methotrexate on the pharmacokinetics and renal excretion of cisplatin.

The kinetics of platinum in plasma and erythrocytes, and its renal excretion, have been examined in five patients with non-small cell carcinoma of the lung, after treatment with cisplatin 50 mg/m2 (Platidiame) and, three weeks later, a combination of 50 mg/m2 cisplatin and 40 mg/m2 methotrexate. The patients were given 0.9% saline 1 l 1 h prior to drug application. Plasma platinum elimination was biphasic with a short initial phase (t1/2 alpha 10-31 min) and a long beta-phase (t1/2 beta 65-91 h). With the exception of increased AUC values in all five patients 0-8 h after the injection, no significant change in the kinetics of platinum in plasma was found after coadministration of methotrexate. In four of the five patients renal platinum excretion was reduced in the first 6 h after administration of methotrexate. The renal clearance of platinum was 50% lower in those four patients 0-3 h after the injection. With the exception of one patient, no signs of nephrotoxicity were observed after combined drug administration. Other toxic effects were mild and showed no increase after the initial administration of methotrexate.

Adult↗
PubMed1988

[Evaluation of the effectiveness of the use of specific hemosorption in acute experimental pancreatitis].

An effective method of plasma proteinases (trypsin, chymotrypsin. elastases) removal in dogs with acute pancreatitis has been suggested. The method is based on plasmasorption, using human urine acid-stable proteinase inhibitor immobilized on sepharose. The procedure of plasmasorption reduces proteinase levels in dogs with acute pancreatitis to 35-70%, with the animal life-span prolonged 8-fold.

Acute Disease↗