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L A Devriese

Publications and source records attributed to L A Devriese.

At least 91 records · Page 5Linked to original sources
PubMed1997

Streptococcus difficile is a nonhemolytic group B, type Ib Streptococcus.

Whole-cell protein electrophoretic analysis of the type strain of Streptococcus difficile (LMG 15799) revealed that this organism was indistinguishable from Streptococcus agalactiae strains. Although LMG 15799T (T = type strain) was originally described as serologically untypeable, we found that this strain was a group B streptococcus belonging to the capsular polysaccharide antigen type Ib group. The biochemical reactivity of S. difficile, which differed from the biochemical reactivity of typical S. agalactiae strains mainly by being less versatile, is similar to the biochemical reactivity of other group B, type Ib streptococci isolated from poikilothermic animals, such as fish and frogs.

Aerobiosis↗
PubMed1997

Streptococcus hyovaginalis sp. nov. and Streptococcus thoraltensis sp. nov., from the genital tract of sows.

Two groups of strains isolated from sows were shown to belong to new sublines in the genus Streptococcus. Based on phenotypic and phylogenetic analyses, we propose that these bacteria should be classified as two new species, Streptococcus hyovaginalis sp. nov. and Streptococcus thoraltensis sp. nov. These two species are found in the genital tract, but the capnophilic species S. thoraltensis may also occur in the intestinal tract of pigs. The type strain of S. hyovaginalis is SHV515 (= LMG 14710), and S69 (= LMG 13593) is the type strain of S. thoraltensis.

Animals↗
PubMed1996

An atypical Staphylococcus aureus intramammary infection in a dairy herd.

An atypical Staphylococcus aureus (S. aureus) strain, isolated in a dairy herd over an 8 month period, was examined. The S. aureus strain was clumping factor negative, weakly heat-resistant deoxyribonuclease positive and produced narrow zones of double haemolysis. In total, 57 quarter infections were observed. Prevalence data per month, calculated as the percentage of quarters infected with this atypical S. aureus strain varied from 7.5% to 17.7% and the new infection rate varied from 0.14 to 0.55. In routine bacteriology, the large number of quarters infected with the atypical S. aureus could easily be misclassified as quarters infected with non-aureus staphylococci.

Animals↗
PubMed1996

Secreted antigens as virulence associated markers in Streptococcus bovis strains from pigeons.

SDS-PAGE and Western blot analysis were performed on the culture supernatant of 7 pigeon S. bovis reference strains belonging to the serotypes 1, 2, 3 and 5. The culture supernatant of highly virulent serotype 1, 2 and 5 strains contained four antigens that were absent in low virulent serotype 3 strains, notably a 185 kDa minor protein band (A) and a triplet (T1) of 70 kDa. The less virulent serotype 3 strains on the other hand contained protein triplets, that had a molecular mass of either 68 kDa (T2) or 74 kDa (T3). The prevalence of A, T1, T2 and T3 was examined in 68 S. bovis strains isolated from healthy pigeons and in 68 S. bovis strains isolated from pigeons that died of S. bovis septicaemia. Six supernatant phenotypes were identified: A-T1 (32 strains), A- T2 (10 strains), A- T3 (7 strains), A+ T1 (84 strains), A+ T2 (1 strain) and A+ T3 (2 strains). Ninety-four percent of the strains lacking the A and T1 proteins were isolated from healthy pigeons, and only 6% were isolated from septicaemia. Strains expressing A and/or T1, however, were isolated form septicaemia in 57% of the cases. These observations may indicate that the A and/or T1 proteins are associated with virulence.

Animals↗
PubMed1996

Taxonomic study of lancefield streptococcal groups C, G, and L (Streptococcus dysgalactiae) and proposal of S. dysgalactiae subsp. equisimilis subsp. nov.

Streptococcus dysgalactiae consists of at least five distinct subgroups on the basis of serogroups, biotypes, and hosts. A chemotaxonomic and phenotypic examination of 80 S. dysgalactiae strains representing the known diversity within this species and 49 reference strains representing all members of the streptococcal pyogenic species group revealed two subpopulations of strains within S. dysgalactiae. The name S. dysgalactiae subsp. dysgalactiae is proposed for strains of animal origin. These strains belong to Lancefield serogroups C and L, are alpha-, beta-, or nonhemolytic, and do not exhibit streptokinase activity on human plasminogen or proteolytic activity on human fibrin. The name S. dysgalactiae subsp. equisimilis is proposed for human isolates. These strains belong to Lancefield serogroups C and G, are beta-hemolytic, and exhibit streptokinase activity on human plasminogen and proteolytic activity on human fibrin.

Animals↗
PubMed1996

A new pathogenic Staphylococcus aureus type in commercial rabbits.

Heavy losses in commercial rabbitries with mainly cutaneous infections were found to be caused by a special Staphylococcus aureus type. This type differs from the previously known rabbit and hare pathogenic strains causing similar infections, mainly in its unique phage type.

Abscess↗
PubMed1996

Presence of vancomycin-resistant enterococci in farm and pet animals.

Enterococcus faecium strains with vanA-mediated glycopeptide resistance were isolated by enrichment culture from the intestines and feces of several animal species, mainly horses and dogs (8% positive), chickens (7% positive), and pigs (6% positive). Other vanA-positive enterococcal strains were identified as E. durans in gallinaceous birds, E. faecalis in a horse, and E. gallinarum in a pheasant. Samples from pigeons, cage birds, and ruminants were negative. It was concluded that vancomycin resistance is widespread among isolates from farm and pet animals.

Animals↗
PubMed1996

Acidification of methyl-alpha-D-glucopyranoside: a useful test to differentiate Enterococcus casseliflavus and Enterococcus gallinarum from Enterococcus faecium species group and from Enterococcus faecalis.

Enterococcus gallinarum and E. casseliflavus are difficult to differentiate from other enterococci, particularly E.faecium. The former two species were found to produce acid from methyl-alpha-D-glucopyranoside in phenol red broth, while E.faecalis strains and strains of the E.faecium species group, including E.faecium, E.durans, E. hirae, and E.mundtii, failed to produce acid from this substrate.

Bacterial Typing Techniques↗
PubMed1995

Characterisation and antimicrobial susceptibility of Enterococcus species from the intestines of ducks in Assam.

The Enterococcus flora of duck intestines consisted of E faecalis and E faecium in ducklings less than eight weeks old and E faecalis, E faecium and E gallinarum in ducks more than eight weeks old. The enterococci were resistant to several antibiotics; chloramphenicol and gentamycin sulphate were the only antibiotics of those tested which were moderately effective. All the Enterococcus species isolates were resistant to the macrolide and lincosamide antibiotics.

Age Factors↗
PubMed1995

Identification of Enterococcus species isolated from foods of animal origin.

Enterococci isolated from a large variety of fresh and prepared foods of animal origin during routine microbiologic control tests in a distribution firm, were identified to species level using API 20 STREP galleries supplemented with conventional tests, rapid ID32 STREP galleries and SDS-PAGE analysis. API 20 STREP tests correctly identified 77% of the strains, mainly Enterococcus faecium and E. faecalis. A simple presumptive identification scheme based on pigmentation, tetrazolium reduction and acid production from mannitol and raffinose identified 90% of the strains. More complex procedures were necessary to identify the remaining strains. Nearly all strains isolated from hard cheeses and prepared cheese-meat combinations were identified as E. faecium while E. faecalis was the most frequent species in crustaceans. In meat and in prepared meat products E. faecium, E. faecalis and less frequently E. hirae/E. durans were found. Three of four E. gallinarum strains were isolated from products containing turkey meat.

Animals↗
PubMed1995

Intracellular survival and multiplication of virulent and less virulent strains of Streptococcus bovis in pigeon macrophages.

The intracellular fate of pigeon S. bovis strains ingested by macrophages was studied in vivo and in vitro. During in vivo experiments, histological and electron microscopical examinations demonstrated numerous cocci, which appeared to be actively multiplying, within splenic macrophages of pigeons experimentally inoculated with a highly virulent S. bovis serotype 1 strain. In pigeons inoculated with a low virulence serotype 3 strain, intracellular cocci were only occasionally observed. For in vitro experiments, pigeon peritoneal macrophages were inoculated with a S. bovis serotype 1 or serotype 3 strain and incubated. Following an initial decrease, an increase in the number of intracellular bacteria was observed in tests performed with the S. bovis serotype 1 strain, demonstrating intracellular multiplication. Macrophages in these experiments had all died after 7 h of incubation, possibly indicating that the intracellular replication of S. bovis resulted in the release of substances toxic for macrophages. In experiments performed with the S. bovis serotype 3 strain, the number of intracellular bacteria continuously decreased, reflecting killing of organisms. Significant changes in the number of adhering macrophages in S. bovis serotype 3 inoculated cultures were not observed. These results indicate S. bovis in pigeons is a facultative intracellular bacterium and intracellular multiplication may be involved in virulence.

Ampicillin↗
PubMed1994

Streptococcus bovis infections in pigeons: virulence of different serotypes.

In a first experiment, the relative virulence for pigeons of 5 strains of S. bovis was assessed by experimental inoculations. Two S. bovis serotype 1 strains, one serotype 2 strain and two serotype 3 strains were examined. One of the serotype 1 strains and the serotype 2 strain were isolated from pigeons that died from septicaemia. The other strains were isolated from cloaca samples of healthy pigeons. For each strain, 10-20 pigeons were intravenously inoculated with 1 x 10(9) CFU. Morbidity after infection with the serotype 1 and 2 strains varied between 75% and 90%. Disease signs included inability to fly, lameness, emaciation, production of slimy, green droppings, polyuria and sudden death. In groups of pigeons inoculated with the serotype 3 strains, morbidity was 0% and 6%, respectively. Results demonstrate that serotype 3 strains are less virulent for pigeons than serotype 1 and 2 strains. In a second experiment, bacteriological and histological examinations were performed on organs of pigeons serially killed between 1 and 10 days after experimental inoculation with an S. bovis serotype 3 strain of low virulence. Results were compared with results of studies carried out with a highly virulent serotype 1 strain. Notwithstanding bacterial spread and replication in various organs of inoculated pigeons, clinical disease was not observed and histological lesions were scarce and of limited extent.

Animals↗
PubMed1994

An unusual outbreak of Streptococcus bovis septicaemia in racing pigeons (Columba livia).

In December 1991, an outbreak of Streptococcus bovis septicaemia occurred in a Belgian pigeon loft where 25 male and 25 female racing pigeons were housed. The main clinical signs included inability to fly and poor breeding results. None of the female pigeons and only one male pigeon was able to fly. Nine affected pigeons were necropsied. Histologically a tenosynovitis of the tendon of the deep pectoral muscle was observed in most of them and S bovis was isolated from the canalis triosseus or the shoulder joints of five of the nine pigeons. The pigeons were successfully treated with ampicillin administered in the drinking water for seven days.

Animals↗
PubMed1994

A simple identification scheme for coagulase negative staphylococci from bovine mastitis.

Coagulase-negative staphylococci from cases of bovine mastitis were identified to species level by using an identification scheme based on a three-plate test system which tested for DNase on DNA agar, for protease on calcium caseinate agar, and for the organism's sensitivity to novobiocin, desferrioxaminine (deferoxamine) and fosfomycin by agar diffusion tests. Testing for the inhibition of Staphylococcus delta haemolysin (Skalka 1991) can replace the protease tests.

Agar↗
PubMed1994

Serotypes and putative virulence markers of Streptococcus suis isolates from cats and dogs.

Thirteen isolates of Streptococcus suis from cats and dogs were characterised. Formamide extracts of 10 of the 13 cultures reacted with group D specific antiserum, and serotyping of the cultures with specific antisera against capsular types 1 to 28 revealed the serotypes 9, 20, 1/2, 22, 26 and 4; the remaining cultures were untypable. In addition one of the cultures reacted with monoclonal antibodies against muramidase-released protein and in parallel with monoclonal antibodies against the extracellular factor (EF) of S suis. The latter reacted with proteins with a molecular weight of more than 150,000 indicating that the strain exhibited EF-related proteins. One of the 13 cultures haemagglutinated erythrocytes from human beings and various animal species and adhered in large numbers to HeLa cells.

Animals↗