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L A Devriese

Publications and source records attributed to L A Devriese.

At least 163 records · Page 9Linked to original sources

Identification and characteristics of staphylococci isolated from lesions and normal skin of horses.

One hundred and twenty eight strains of Staphylococcus from lesions, mostly of the skin, in horses were identified and compared with 29 strains isolated from the healthy skin. The pathogenic species Staphylococcus aureus, S. intermedius and S. hyicus were found almost exclusively in lesions. Other species such as S. xylosus and S. sciuri were more frequently found on the healthy skin than in lesions. The S. aureus strains formed a very heterogeneous collection. Many of these strains were staphylokinase positive and rapidly coagulated bovine plasma. Such strains are rarely found in other animals and man.

Abscess↗

Identification of coagulase-negative staphylococci from farm animals.

The species identify of 661 strains of coagulase-negative staphylococci isolated from the skin and nares of cattle, pigs, poultry, goats and sheep was determined. They belonged either to the novobiocin-sensitive species Staphylococcus hyicus, Staph. simulans, Staph. epidermidis, Staph. haemolyticus and Staph. warneri or to the novobiocin-resistant species Staph. sciuri, Staph. lentus, Staph. xylosus, Staph. cohnii, Staph. saprophyticus and Staph. gallinarum; twenty-one strains remained unidentified. The staphylococcal flora of the farm animals studied differed markedly from that associated with man; several species which do not occur in man were isolated and novobiocin-resistant strains, which occur infrequently in man, were present in large numbers in animals. Two simplified schemes for the identification of staphylococci from farm animals and man are presented.

Animals↗

A note on the isolation of Staphylococcus aureus from raw minced meat.

Conventional Baird-Parker medium (BP) and three modified BP-type media were tested for their suitability to detect and to enumerate Staphylococcus aureus in raw minced meat. BP with pig plasma gave the most rapid results without the need for extensive confirmatory tests. A method which combined a liquid modification of BP medium with BP agar supplemented with acriflavine, polymyxin and sulphamezathine, was cheaper, more sensitive, and did not necessitate additional identification. Conventional BP incubated at 37 degrees and 43 degrees C yielded less satisfactory results.

Animals↗

Biotypes of enteropathogenic Escherichia coli strains from rabbits.

Forty-five enteropathogenic (enteropathogenic Escherichia coli-like) strains isolated in commercial rabbit farms were subdivided into four biotypes with the help of six carbohydrate fermentation tests, ornithine decarboxylase tests, and motility tests. E. coli strains which caused neonatal diarrhea in baby rabbits belonged to a single biotype, biotype 1, and displayed the same serotype antigens. Biotype 2 strains provoked diarrhea of variable severity in weaned rabbits and were also pathogenic for baby rabbits under laboratory conditions. Biotype 1 and biotype 2 strains resembled each other in many respects. Biotype 3 and biotype 4 (represented by a single strain) were highly pathogenic for weaned rabbits, but they provoked no symptoms or lesions in baby rabbits. Biotype 3 formed a homogeneous group of nonmotile strains which possessed the O15 antigen. Forty-two strains from nondiarrheic rabbits constituted a very heterogeneous collection of biotypes, most of which could easily be differentiated from the pathogenic types. Biotyping can be used to recognize rabbit pathogenic strains.

Animals↗

Cutaneous staphylococcosis in rabbits.

An outbreak of cutaneous staphylococcosis caused high mortality among newborn and very young rabbits. The most typical lesions were exudative dermatitis in the youngest, subcutaneous abscesses in rabbits of all ages and mastitis in lactating does. Generalised staphylococcosis was a frequent secondary manifestation of the disease.

Animals↗

Identification and characterization of staphylococci isolated from cats.

Staphylococcus simulans was found to be the most common staphylococcal species present on the healthy skin and in the nares of cats. S. aureus was the most frequent Staphylococcus species in lesions. Most of the S. aureus strains showed the characteristics of the human ecovar. Other strains isolated formed a heterogenic collection in which not less than 13 species were represented. One of these, S. epidermidis, was almost exclusively associated with lesions. It was concluded that coagulase-positive staphylococci are not endogenous to cats. Staphylococci from other sources, most frequently from man, are often associated with lesions in these animals.

Animals↗

A simplified system for biotyping Staphylococcus aureus strains isolated from animal species.

A biotyping system for Staphylococcus aureus strains is proposed which is a simplified version of biotyping procedures described in the literature. It differentiates Staph. aureus strains from man and animals into host-specific ecovars and biotypes which are not host-specific. With the help of tests for beta-haemolysin, staphylokinase , coagulation of bovine plasma and the crystal-violet reaction, the origin of many but not all Staph. aureus strains can be determined: 604 of 809 strains from man, poultry, cattle, pigs, goats, rabbits and foods could be alloted to four ecovars which are typically associated with man, poultry, sheep and goats and cattle. The other strains belonged to five non-host specific biotypes.

Animals↗

Observations on the in vitro sensitivity and resistance of Gram positive intestinal bacteria of farm animals to growth promoting antimicrobial agents.

Results of qualitative in vitro sensitivity tests made with growth promoting antimicrobial agents on clostridia, lactobacilli and enteric streptococci, isolated from caeca of pigs, cattle and poultry, are summarized. The compounds were active in vitro against most of the 19 bacterial species studied. However, their activity spectra differed markedly. Only four bacterial species were naturally sensitive to all agents. The natural resistances, typical for many species and compounds, could be differentiated from the acquired resistances found with all growth promotors except avoparcin. High resistance and complex patterns of cross-resistance involving one or more of the macrolide, lincosamide and streptogramin antibiotics were detected.

Animals↗

Effects of erythromycin-inactivating Lactobacillus crop flora on blood levels of erythromycin given orally to chicks.

Germ-free, day-old chickens were colonized with a Lactobacillus fermentum strain isolated from poultry which was able to inactivate in vitro erythromycin and other macrolide and lincosamide antibiotics. Similar control chickens were colonized with a non-degrading L. fermentum strain. Only in chickens colonized with the non-degrading strain were blood levels considered to be therapeutically active achieved with erythromycin in the drinking water. Five groups of broiler chickens of different age originating from three farms all possessed an erythromycin-degrading crop flora. In none of these were therapeutic blood levels attained when erythromycin was given in the drinking water.

Administration, Oral↗

Pathogenic properties of Escherichia coli strains isolated from diarrheic commercial rabbits.

Thirty-two different strains of Escherichia coli isolated from diarrheic commercial rabbits showing intestinal attachment of bacilli were studied. None of the strains produced thermostable or thermolabile enterotoxins, and none was invasive. Strains isolated from suckling rabbits attached in vitro to the brush borders of intestinal villi, whereas strains from weanling rabbits did not. After experimental infection of 5-week-old rabbits, the 26 strains isolated from weaned diarrheic rabbits attached to the epithelium of ileum, cecum, and colon, whereas only slight attachment was found after infection with the six strains isolated from suckling diarrheic rabbits. The former strains induced diarrhea in 87% of the rabbits, whereas the latter induced diarrhea in only 9% of inoculated rabbits. E. coli isolated from healthy rabbits did not cause diarrhea. Strains isolated from diarrheic suckling rabbits all belonged to serotype O109:K-:H2, whereas strains from diarrheic weaned rabbits belonged to at least eight different serogroups. It is suggested that two different mechanisms of E. coli enteropathy might exist in rabbits.

Animals↗

Susceptibility of clostridia from farm animals to 21 antimicrobial agents including some used for growth promotion.

The minimal inhibitory concentrations of 21 antimicrobial agents were determined by an agar dilution method against 68 strains of 18 Clostridium species isolated from caeca of pigs, cattle and poultry. Of the therapeutically used antibiotics, chloramphenicol was most active in vitro followed by penicillin G, the lincosamides and tetracycline. Penicillin-resistant Cl. butyricum strains produced a beta-lactamase. Avoparcin, carbadox, monensin, nitrovin and virginiamycin were the most effective of the growth promoting antimicrobial agents. Many clostridial species were naturally resistant to flavomycin and Cl. sporogenes was naturally resistant additionally to bacitracin, tiamulin, the lincosamides and virginiamycin component M. The macrolide, lincosamide and streptogramin resistant strains showed four different patterns of resistance to the antibiotics. One of these resistance patterns was the natural resistance of Cl. sporogenes to the lincosamides and virginiamycin component M which is a streptogramin group A antibiotic.

Animals↗

Staphylococcus hyicus in skin lesions of horses.

Staphylococcus hyicus (subspecies hyicus) was isolated as the only pathogenic organism from two independent cases of dermatitis of the lower parts of the limbs (grease heel) in horses. The organism was recovered together with other pathogenic staphylococci from similar conditions in two other horses of different origins. These conditions were characterised by epidermolysis, alopecia and crust formation. They responded quickly to antibiotic treatment. The organism was also isolated from a long standing case of "summer eczema" which healed without antibiotic treatment, and from a horse with dermatophilosis (streptotrichosis, Dermatophilus congolensis infection). Experimentally, Staph hyicus caused epidermolysis, exudation and inflammation in the superficial layers of the skin.

Actinomycetales Infections↗

Biotypes, serotypes and phage types of caprine strains of Staphylococcus aureus.

Nine of 11 caprine strains of Staphylococcus aureus examined belong to biotype C. They were typable serologically using antisera developed for typing human strains of Staphylococcus aureus. H2 was found to be the dominant thermostable antigen whilst 1 and 0 were the most common thermolabile antigens. Some strains possessed protein A and poly A beta (beta-glucosaminyl ribitol teichoic acid). Phage typing of the strains was achieved with an international set of phages for typing human strains of Staphylococcus aureus and they belong to phage groups II, and the mixed group.

Animals↗

Branched Mycobacterium-like organisms from swine lymph nodes.

Branched, weakly acid fast organisms were isolated from 5 of 112 caseous lymph nodes derived from slaughter-house pigs. In two cases they were associated with typical Mycobacterium avium strains. The bacteria differed from all other mycobacteria known at present. Limited experiments in pigs with one strain failed to disclose a possible pathogenic role in the host species although this strain was pathogenic for mice. Tests with this and three other strains showed that all four were apathogenic for chickens.

Abattoirs↗

Susceptibility of fecal streptococci of poultry origin to nine growth-promoting agents.

The minimal inhibitory concentrations of nine growth-promoting agents were determined by an agar-dilution method against 66 bile-tolerant streptococcal (8 Streptococcus faecalis, 23 Streptococcus faecalis subsp. liquefaciens, 15 Streptococcus faecium, and 20 carboxyphilic streptococci) strains isolated from the ceca of 52 chickens on 19 farms. Avoparcin was equally active on all groups. The natural susceptibilities against the other substances differed among the groups studied. Bacitracin and virginiamycin were more active on S. faecium and S. faecalis than on S. faecalis subsp. liquefaciens; lincomycin and the macrolide antibiotics were more active on S. faecium than on the other groups; and flavomycin was active on all groups except S. faecium. High percentages of acquired resistance were noted in all groups against bacitracin, lincomycin, and the macrolide antibiotics, oleandomycin, spiramycin, and tylosin. Resistance to nitrovin was found only among the S. faecalis and S. faecium groups.

Animals↗