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Biomedical subjects

L A Gracheva

Publications and source records attributed to L A Gracheva.

16 recordsLinked to original sources
PubMed1991

[Complement 3 in hypertension and arteriosclerosis].

The serum levels, residual functional activity (RFA), phenotype of complement component 3 (C3) were determined in 20 patients with hypertensive disease (HD), 11 with documented coronary atherosclerosis (CA), and 11 with HD + CA. The sera from 21 apparently healthy subjects were used as a control. There was a higher frequency of the allotype C3F in the HD, CA, and HD + CA groups (0.400, 0.417, and 0.364, respectively) than in the controls. The HD + CA group showed a significant (p less than 0.05) decrease in RFA of C3 as compared to the HD and CA groups. There were significant differences in the serum C3 concentrations and RFA in allotype C3F carriers between the patients with CA and HD. The findings suggest that allotype C3F carriage may be a factor predisposing to accelerated progression of CA in HD.

Adult↗
PubMed1991

[Effects of atrial natriuretic factor on proliferative response and natural cytotoxicity of human lymphocytes].

Effect of synthetic analogues of atrial natriuretic factor (ANF) on proliferative response and natural cytotoxic lymphocytes of human subjects was investigated in vitro. ANF-III and ANF-IV increased blast transformation lymphocytes induced by a Con-A suboptimal dose. The increase of cells activity was comparable with the effect of interleukin-2, added at a dose of 50-100 unit/ml.

Atrial Natriuretic Factor↗
PubMed1990

Anticardiolipin antibodies in Sneddon's syndrome.

We studied 24 patients (18 women, 6 men), aged 29 to 54, with Sneddon's syndrome. The clinical picture of Sneddon's syndrome was characterized by cerebrovascular disorders, livedo reticularis, disturbance of peripheral circulation, arterial hypertension, cardiac pathology (ischemic heart disease, heart murmurs), complicated obstetric history in women, and disturbed sexual function in men. In 6 of 17 examined patients with Sneddon's syndrome there was a high concentration of anticardiolipin antibodies (ACA) but no antibodies to native DNA and LE cells. The course of the disease in the patients with a high ACA level, when compared with normal ACA level patients, was characterized by a more rapid progression and more severe clinical manifestations. The study demonstrates the similarity of clinical symptoms and immunologic disturbances in Sneddon's syndrome and the antiphospholipid syndrome and suggests the importance of ACA in the pathogenesis of some cases of Sneddon's syndrome.

Adolescent↗
PubMed1989

[Study of lymphocyte subpopulations and the expression of activation markers in diabetes mellitus types 1 and 2].

Diabetic patients with the disease duration less than 1 year (group 1 eight patients with diabetes mellitus type I and group 2 seven patients with diabetes mellitus type II) and 8 healthy donors were examined for subpopulations of lymphocytes (CD 3-, CD 4-, CD 8-, CD 20-positive cells, CD4/CD8), expression of activation markers on peripheral blood mononuclears investigated with monoclonal antibodies of BMA and OKT series, and serum neopterin concentration. Group I patients had low CD4/CD8 and increased number of CD 8 cells, 1a/DR-positive lymphocytes (28.6 +/- 7%), OKT9-positive lymphocytes (8.0 +/- 4.7%), activated neopterin synthesis registered neither in group 2 patients nor donors. The number of CD 3 and CD 4 cells was similar in the diabetics and donors. B-lymphocyte level in group 1 patients was on the decrease. Unbalance in lymphocyte subpopulations, increased expression of activation markers and of serum neopterin can be noted in viral infection reflecting impairment of immunoregulating mechanisms in diabetes mellitus type I.

Adolescent↗
PubMed1989

[Disorders of humoral immunity in hypertension].

Examination included 51 patients with essential hypertension (EH) of an uncomplicated course, labile (IB-IIA stage, according to A. L. Myasnikov's classification) and stable (IIB stage) hypertension. Clinical characteristics were given to the stages and duration of EH, body weight of the patients, arterial hypertension (AH) heredity and the AP level. Immunological examination included determination of the concentration of the basic classes of immunoglobulins IgG, IgA, IgM, circulating immune complexes (CIC), concentration of IgE and beta 2-microglobulins. It was revealed that EH development is attended by an increased concentration of immunoglobulins, primarily of IgA (23 per cent), IgE (31 per cent) and CIC (21 per cent), which is associated, to a certain degree, with a factor of AH hereditary aggravation.

Adult↗
PubMed1988

[Immunoregulatory disorders and activation of the immune system in dilated cardiomyopathy].

The level of immunoregulatory lymphocytic subpopulations (CD3-, CD4-, CD8-cells, CD4/SD8 ratio, IaDr-positive cells, SD16-cells), lymphocyte natural cytotoxicity (NCT), the concentration of serum interferon (IF), beta 2-microglobulin (beta 2-M) and neopterin were investigated in 30 patients with dilatative cardiomyopathy (DCMP). Immunoregulatory lymphocytic subpopulations were defined by indirect immunofluorescence using monoclonal antibodies to differentiating markers of BMA and OKT series. A decrease in SD4/SD8 ratio less than 1 was observed in 10 patients, an increase in SD4/SD8 ratio over 2.3 was observed in 5 patients. A significant rise of IaDr-positive cells, a decrease in NCT, an increase in the concentration of IF, beta 2-M and neopterin were observed. There was correlation of an increase in IF concentration, the number of IaDr-positive cells and an increase in the level of neopterin and beta 2-M. Signs of activation of the immune system were more frequently noted in patients with a decreased (less than 1) or increased CD4/CD8 ratio. The role of disorders of immunoregulation and activation of the immune system in DCMP pathogenesis was discussed.

Adolescent↗
PubMedDate not supplied

[Isolation of type 71 enterovirus from patients with a poliomyelitis-like disease during an outbreak in Bulgaria].

Virological examinations of 72 specimens obtained during an outbreak of a poliomyelitis-like disease in Bulgaria, 1975, yielded 23 strains of enterovirus type 71 (E71) and 5 strains of enteroviruses of other types. The viruses were isolated in green monkey kidney cell cultures, in newborn cotton rats, newborn white mice and in monkeys. Most of E71 strains (16 out of 23) were isolated from the autopsy materials (brains and spinal cords) from 9 children dying of poliomyelitis, meningoencephalitis, and encephalitis. The remaining E71 strains and all the strains of other enterovirus serotypes, with the exception of ECHO7, were isolated from stool specimens of children with aseptic meningitis and their contacts. The antigenic similarity of the group of Bulgarian strains and their relationship with the prototype BrCr strain of E71 was established. The conclusion is that E71 was the causative agent of an outbreak of a poliomyelitis-like disease in Bulgaria.

Animals↗
PubMedDate not supplied

[Use of monoclonal antibodies for the intratypic differentiation of strains of poliomyelitis type I virus].

Six clones of mouse hybridomas producing highly specific antibodies to the vaccine LSc 2ab strain of poliomyelitis virus type I have been derived. The clone 10C5 antibodies were used for intratypic serodifferentiation of 75 poliomyelitis virus type I strains from the collection of the Institute of Poliomyelitis and Viral Encephalitides of the USSR Acad. Med. Sci. which had been isolated in the USSR from children with paralytic poliomyelitis in 1950-1983. General large-scale vaccination campaigns for children using live poliomyelitis vaccine (SLV) had started in 1959. Among 12 strains isolated in 1950-1958, only 2 strains could be neutralized with clone 10C5 antibody. These 2 strains were evidently of the vaccine origin since they had been isolated in 1957 in Leningrad where at that time first large-scale trials of SLV had already been initiated (Smorodintsev, A.A. in: "Live Vaccine against Poliomyelitis", Leningrad, 1960, 42-60). In the period after the beginning of large-scale vaccination campaigns (1959-1983), 11 out of 26 strains and 25 out of 36 strains isolated in areas of the moderate and hot climate, respectively, showed antigenic relationship with the vaccine LSc 2ab strain. The portion of vaccine-like strains isolated in different years beginning since 1959 was sufficiently stable comprising about half of the total number of poliomyelitis virus type I strains examined.

Animals↗
PubMedDate not supplied

[Enterovirus type 71 pathogenicity for laboratory animals].

Enterovirus type 71 (E71) strains isolated from specimens of the brain and feces of children during an outbreak of poliomyelitis-like infection in Bulgaria in 1975 produce paralytic disease in newborn and adult cotton rats, newborn white mice, and monkeys. By the type of the myo- and neurotropic properties manifested in animal experiments the Bulgarian E71 strains are very close to neuropathogenic strains of Coxsackie A7, A14, and A16 viruses. The infection induced by the Bulgarian E71 strains in green monkeys was by clinical symptoms (tremor, convulsions, death), the speed of the development of the process and the type of morphological lesions particularly similar to the clinical and morphological manifestations of infection observed in most severe cases in children during the outbreak in Bulgaria. Inoculation of newborn and adult cotton rats, newborn white mice and Macaca rhesus monkeys with large doses of the prototype BrCr strain of enterovirus 71 (10(5.0)--10(6) TCD50) caused no clinically manifest disease of the animals.

Animals↗