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Biomedical subjects

L A Profenno

Publications and source records attributed to L A Profenno.

2 recordsLinked to original sources

Microwave-specific heating affects gene expression.

The effects of low-level microwave radiation on gene expression in Escherichia coli have been examined in a sensitive model. We confirm the previously reported existence of an increase in beta-galactosidase expression by microwave radiation--an increase not duplicated by bulk heating. However, the effect was not frequency dependent and appeared to be due to heating effects peculiar to microwaves. These results indicate that small thermal gradients may be a source of biological effects of non-ionizing radiation.

Escherichia coli

Sensitive model with which to detect athermal effects of non-ionizing electromagnetic radiation.

To clarify the potential of non-ionizing electromagnetic radiation to cause biological effects by athermal mechanisms, and to initiate elucidation of those mechanisms, a model system amenable to scrutiny at the molecular level has been designed and characterized. Assessment of beta-galactosidase activity in E. coli JM101 containing the plasmid pUC8 provides a sensitive assay with many important advantages. The ability to examine at the molecular level each of the processes involved in producing beta-galactosidase should permit elucidation of the molecular mechanism(s) that give rises to an observed effect.

Animals