Biomedical subjects

L A Reinhardt

Publications and source records attributed to L A Reinhardt.

5 recordsLinked to original sources

PubMed2001

Regiospecificity assignment for the reaction of kanamycin nucleotidyltransferase from Staphylococcus aureus.

Aminoglycoside nucleotidyltransferases catalyze the transfer of a nucleoside monophosphoryl group from a nucleotide to a hydroxyl group of an aminoglycoside antibiotic. Kanamycin nucleotidyltransferase [ANT (4',4' ')-I] from Staphylococcus aureus confers resistance to numerous aminoglycosides with a 4' or 4' ' hydroxyl group in the equatorial position. The synthesis of m-nitrobenzyl triphosphate, a new substrate of kanamycin nucleotidyltransferase, is reported. The kanamycin nucleotidyltransferase catalyzed reaction of kanamycin A with m-nitrobenzyl triphosphate is 2 orders of magnitude slower than that with ATP. The MALDI-TOF spectra of the purified products of both reactions revealed that kanamycin A was modified only at one position. The regiospecificity of the reaction catalyzed by kanamycin nucleotidyltransferase of kanamycin A with either ATP or m-nitrobenzyl triphosphate was determined directly by one- and two-dimensional hetero- and homonuclear NMR techniques. The site of the modification was unambiguously assigned to the 4' hydroxyl of kanamycin A; thus, the products formed are 4'-(adenosine-5'-phosphoryl)-kanamycin A and 4'-(m-nitrobenzyl phosphoryl)-kanamycin A. This eliminates the uncertainty concerning the point of modification since this could not be determined from the crystal structure of the enzyme with bound MgAMPCPP and kanamycin A [Pedersen, L. C., Benninig, M. M., and Holden, H. M. (1995) Biochemistry 34, 13305-13311].

Adenosine Monophosphate↗

PubMed1985

Prostaglandins and nicotinate-provoked increase in cutaneous blood flow.

The mechanism of topically applied methyl nicotinate-induced local cutaneous erythema was studied in normal human subjects. Aqueous methyl nicotinate (0, 0.1, 1.0, 10.0, and 100 mmol/L) was applied to the volar forearms in quadruplicate after oral pretreatments with 25 mg doxepin hydrochloride, 600 mg ibuprofen, 50 mg indomethacin, 975 mg aspirin, and lactose placebo. The cutaneous vascular response was monitored by laser Doppler velocimetry. Although doxepin did not affect the cutaneous vascular response to methyl nicotinate, indomethacin, ibuprofen, and aspirin significantly suppressed the response. Because indomethacin, ibuprofen, and aspirin have different chemical structures, the common property of inhibition of the response to methyl nicotinate may be assigned to their common pharmacologic action, i.e., inhibition of prostaglandin bioformation.

Administration, Oral↗

PubMed1983

Lichenoid eruption produced by captopril.

Captopril, an oral active dipeptidylcarboxypeptidase inhibitor with antihypertensive properties, has been reported to have the following cutaneous side effects: macular and papular skin eruptions, urticaria, angioedema, mouth ulcers, pemphigus, and pityriasis rosea-like eruptions. Here, to the best of our knowledge, is the first case in which a pityriasis rosea-like eruption evolved into a lichenoid drug eruption. Also discussed is the remarkable similarity in the side effects of captopril, gold compounds, d-penicillamine, and organic mercurials.

Captopril↗

PubMed1983

Pityriasis rubra pilaris as the initial manifestation of leukemia.

The association of pityriasis rubra pilaris (PRP) with an underlying disease is unusual. A case of a PRP-like eruption presenting as the initial manifestation of acute stem cell leukemia is reported. Other noncutaneous diseases previously reported in association with PRP are reviewed. Other cutaneous lesions associated with leukemia are also briefly described.

Acute Disease↗

PubMed1983

Buschke-ollendorff syndrome.

Buschke-Ollendorff syndrome or dermatofibrosis lenticularis disseminata with osteopoikilosis is a rare autosomal dominant condition characterized by focal sclerotic bone dysplasia and associated connective tissue nevi. The following case report not only illustrates classic findings in this disease but also demonstrates previously reported, but little known features.

Adult↗