PubMed Health⌕ Search

Biomedical subjects

L A STERNBERGER

Publications and source records attributed to L A STERNBERGER.

15 recordsLinked to original sources
PubMed1956

Studies on non-precipitating antibody. I. The conversion of non-precipitating antibody to a precipitin-like material in vitro.

Brief exposure of serum to alkali in the cold seems to convert non-precipitating antibody to a precipitin-like material. This was disclosed by the following lines of evidence. 1. Immune sera, after addition of antigen and removal of the ensuing precipitates, yielded after alkali treatment more antibody-antigen complex than did sera to which no antigen was added. 2. When immune sera were depleted of precipitating antibody, treated by alkali, and antibody-antigen complex was allowed to precipitate, the resulting supernates formed additional prescipitates upon admixture of specific antigen. No precipitation occurred upon addition of non-specific protein to the treated supemates or the addition of specific antigen to depleted sera not treated by alkali. 3. Alkali-treated immune sera precipitated more antigen than untreated sera.

Antibodies↗
PubMed1956

Studies on nonprecipitating antibody. II. Circulating antibody-antigen complex following repeat injections of antigen.

Reinjection of antigen into immunized rabbits usually results in an immediate increase of circulating antibody-antigen complex. This immediate formation of complex seems to depend upon the presence of non-precipitating antibody before reinjection. The complex disappears by the 2nd day after reinjection and reappears between the 4th to 7th day. This is earlier than its first appearance after a primary injection and may indicate an anamnestic response of the non-precipitating antibody responsible for the formation of circulating antibody-antigen complex.

Animals↗
PubMed1953

The estimation of circulating antibody-antigen complex.

Immune sera were subjected to treatment with alkali in the cold to cause dissociation of antigen-antibody complexes. Precipitates and a capacity to fix complement developed in some of these sera subsequent to such treatment. The specific immunologic nature of these phenomena and their observation in relation to disappearance of free antigen and appearance of free antibody in circulation are discussed. The phenomena observed appear to be consistent with the assumption that a circulating antibody-antigen complex is revealed as a result of treatment.

Animals↗