[Experimental substantiation of the use of ligand-free albumin in burn toxemia].
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Adolescent↗
Biomedical subjects
L A Sakhno
Publications and source records attributed to L A Sakhno.
Basic studies of local adsorption in burn treatment.
The model of a full-thickness thermal burn in rats was used to demonstrate that the application of adsorptive dressings made of a fibrous activated carbon material (ACFM) after early excision of a burn crust exerts a considerable influence on the development of both local and general symptoms of burn disease. Three days after the excision a water content in underlying tissues falls from 84.81 +/- 0.62% to 54.47 +/- 4.2%, the wound surface area decreases by 10%, while this surface area under gauze bandages increases by 6.3%. The local adsorption results in lower activity of proteolytic serum enzymes and toxicity of blood serum in burned animals as well as in the positive dynamics of changes in functional activity of the immune system. By the 14th day the T-cell activity in animals with adsorptive carbon dressings was by 3.2 times higher than that in the animals with gauze bandages. The level of B-cell response if ACFM is applied exceeds normal values to a great extent, starting from the first days after the excision of a burn crust.
Animals↗
Application sorption: experience in clinical use and prospects of development.
The analysis of experimental - clinical results obtained during the last decade in the field of application of adsorptive carbon dressings in wound and burn therapy is given. Possible mechanisms of the therapeutic effect of this material are discussed, the feasibility of their application during different phases of wound process and burn disease treatment is demonstrated. The local absorption may be independent efficient method of treatment in the case of surface burns and wound with a small area of injury and provide a pronounced detoxifying effect in the case of suppurative-septic complication of a mechanical trauma and burns.
Bandages↗
[Experimental substantiation of the early use of applied sorption in treating wounds].
In the experiments on rabbits with bilateral musculocutaneous wound of a thigh, it is shown that the early use of the "Dnepr MN" activated carbon fibrous material (ACFM) intended for medical purposes provides timely haemostasis in the damaged tissues, reduces blood loss, prevents the development of traumatic edema, decreases the intensity of inflammatory process in a wound, facilitates rapid and uncomplicated healing of wounds under the artificial crust. The treatment-and-prophylactic effectiveness of ACFM is associated with its ability for rapid and irreversible sorption of biologically active substances forming in the wound tissues, and as well with protection of a wound against secondary infection.
Absorption↗
[Effect of applicative-sorptive therapy on the activity of various enzymes and inhibitors of the kallikrein-kinin system in traumatic disease].
On the model of musculocutaneous wound in rabbits, the effect of applicative sorption with the use of activated carbonic fibrous materials on the course of traumatic disease was studied. The total esterase activity of the blood serum, activity of alpha 1-inhibitor of proteinases, kallikrein content in the blood serum were defined. It is shown, that changes in biochemical indices are in agreement with the findings of clinical observations and outcome of traumatic disease. The use of activated carbonic fibrous material at the early period of traumatic disease contributes to its favourable course, prevents the development of complications and death of the animals from sepsis.
Animals↗
[Antimetastatic action of bacterial endotoxins and changes in the activity of the enzymes of purine metabolism in macrophages].
The antimetastatic action of bacterial endotoxins (BET), E. coli 0111:B4, B in particular, as well as their influence on the adenosine deaminase and 5'-nucleotidase activity were studied in peritoneal macrophages of mice bearing lung Lewis carcinoma. BET inhibition of lung metastasis growth was found to be due to such changes in macrophage adenosine metabolism, that testifies to the rise of their functional activity. The change in the level of macrophage purine metabolism can be considered as an important evidence of the effectiveness of the drugs capable to inhibit the metastasis growth.
5'-Nucleotidase↗
[Immunological characteristics of a modifying effect of bacterial endotoxins on the growth of experimental tumors].
Two experimental models have been elaborated for E. coli 0111:B4 endotoxin effects on the growth of transplanted murine sarcomas induced by Meth A. A correlation is shown between stimulatory and inhibitory effects of endotoxin on the tumour growth and functional activity of T- and B-lymphocytes registered by in vivo tests.
Animals↗
[Hygienic evaluation of a set-up for continuous pouring of steel of a curvilinear type].
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Alloys↗
[Activity of the corn Spm transposon system in transgenic plants Orychophragmus violaceus (L.) O.E. Schulz obtained by both direct transfer of DNA to protoplasts and agrobacterial transformation of root explants].
Transposon mediated insertional mutagenesis is one of the approaches for the unique gene cloning. A wild species of Cruciferae family Orychophragmus violaceus (L.) O.E. Schulz, which is of interest for practical breeding as a donor of improved plant oil, was an object of the investigation. Plasmid construction used in the experiments included selective NPT II gene, reported GUS gene serving as an excision marker, structural BAR gene located within the dSpm element and Spm transposase. The GUS gene of this plasmid had not his own promoter and became functional only after Spm-transposition. Transformed Orychophragmus violaceus (L.) O.E. Schulz. plants were obtained by direct mesophyll protoplast transformation as well as Agrobacterium tumefaciens-mediated root explant transformation. Gene transfer and the transposition event were confirmed by the GUS activity and the PCR analysis. Relative transformation efficiency using protoplasts was 5.8%.
Agrobacterium tumefaciens↗
[Obtaining and analysis of intergeneric somatic hybrids between Brassica napus and "albino" line of Orychophragmus violaceus].
The Orychophragmus violaceus chlorophylldefective line of "albino" type has been obtained by spectinomycin treatment. Somatic hybridization between Orychophragmus violaceus and Brassica napus was performed by fusion of green mesophyll protoplasts of rape and callus protoplasts of the O. violaceus "albino" line. Near two hundred of regenerant plants were selected according to the regeneration type and ability to become green, and were determined as hybrids. Chloroplast DNA in selected hybrids was identical to rape chlDNA, which was confirmed by the PCR-RFLP analysis of plastid DNA fragments. Fragments of hybrid mitochondrial DNA analyzed by the PCR-RFLP analysis were identical to fragments of O. violaceus. The nuclear genome of the majority of hybrids was represented by the O. violaceus genome, which was demonstrated by analyses of isoenzymes, DNA telomeric sequences, ribosomal and satellite DNAs, and the RAPD analysis. The cytogenetic analysis of a number of lines has shown variability in the number of chromosomes in the obtained lines.
Brassica napus↗