Biomedical subjects

L Arend

Publications and source records attributed to L Arend.

9 recordsLinked to original sources

PubMed2001

Parallel detection of violations of color constancy.

The perceived colors of reflecting surfaces generally remain stable despite changes in the spectrum of the illuminating light. This color constancy can be measured operationally by asking observers to distinguish illuminant changes on a scene from changes in the reflecting properties of the surfaces comprising it. It is shown here that during fast illuminant changes, simultaneous changes in spectral reflectance of one or more surfaces in an array of other surfaces can be readily detected almost independent of the numbers of surfaces, suggesting a preattentive, spatially parallel process. This process, which is perfect over a spatial window delimited by the anatomical fovea, may form an early input to a multistage analysis of surface color, providing the visual system with information about a rapidly changing world in advance of the generation of a more elaborate and stable perceptual representation.

Color Perception↗

PubMed2000

Low chloride stimulation of prostaglandin E2 release and cyclooxygenase-2 expression in a mouse macula densa cell line.

Reducing luminal NaCl concentration in the macula densa region of the nephron stimulates renin secretion, and this response is blocked by a specific inhibitor of cyclooxygenase-2 (COX-2) (Traynor, T. R., Smart, A., Briggs, J. P., and Schnermann, J. (1999) Am. J. Physiol. Renal Physiol. 277, F706-710). To study whether low NaCl activates COX-2 activity or expression we clonally derived a macula densa cell line (MMDD1 cells) from SV-40 transgenic mice using fluorescence-activated cell sorting of renal tubular cells labeled with segment-specific fluorescent lectins. MMDD1 cells express COX-2, bNOS, NKCC2, and ROMK, but not Tamm-Horsfall protein, and showed rapid (86)Rb(+) uptake that was inhibited by a reduction in NaCl concentration and by bumetanide or furosemide. Isosmotic exposure of MMDD1 cells to low NaCl (60 mm) caused a prompt and time-dependent stimulation of prostaglandin E(2) (PGE(2)) release that was prevented by the COX-2 specific inhibitor NS-398 (10 microm). Reducing NaCl to 60 and 6 mm for 16 h increased COX-2 expression in a chloride-dependent fashion. Low NaCl phosphorylated p38 kinase within 30 min and ERK1/2 kinases within 15 min without changing total MAP kinase levels. Low NaCl-stimulated PGE(2) release and COX-2 expression was inhibited by SB 203580 and PD 98059 (10 microm), inhibitors of p38 and ERK kinase pathways. We conclude that low chloride stimulates PGE(2) release and COX-2 expression in MMDD1 cells through activation of MAP kinases.

Animals↗

PubMed1998

Tubuloglomerular feedback: new concepts and developments.

Luminal [NaCl] at the macula densa (MD) has two established effects: regulation of glomerular arteriolar resistance through tubuloglomerular feedback (TGF) and control of renin secretion. TGF acts as a minute-to-minute stabilizer of distal salt delivery, thereby minimizing the impact of random perturbations in filtration and absorption forces on NaCl excretion. During long-lasting perturbations of MD [NaCl], control of renin secretion becomes the dominant function of the MD. The potentially maladaptive effect of TGF under chronic conditions is prevented by TGF adaptations permitting adjustments in glomerular filtration rate to occur. TGF adaptation is mechanistically coupled to the endpoint targeted by chronic deviations in MD [NaCl], the rate of local and systemic angiotensin II generation. Studies of TGF in transgenic mice are expected to provide further insights into the mechanisms mediating between luminal [NaCl] and afferent arterioles. TGF responses are virtually abolished in mice in which either the AT1A gene or the angiotensin converting enzyme gene is rendered nonfunctional by homologous recombination. In contrast, TGF responses are unaltered in nitric oxide synthase I knockout mice. Thus, an intact renin-angiotensin system appears to be critical for the TGF signaling pathway.

Animals↗

PubMed1998

Inhibition of adenosine-1 receptor-mediated preglomerular vasoconstriction in AT1A receptor-deficient mice.

The effect of the adenosine type 1 receptor agonist N6-cyclohexyladenosine (CHA) on glomerular vascular reactivity was studied in male angiotensin II type 1A (AT1A) receptor knockout mice (9). Vascular reactivity was assessed as the response of stop-flow pressure (PSF) to infusion of CHA into loops of Henle using micropuncture techniques. In AT1A +/+ mice at ambient arterial blood pressure (96.7 +/- 2.8 mmHg), the presence of CHA (10 (-5) M) in the perfusate increased PSF responses from 6.8 +/- 0.6 to 14.3 +/- 0.9 mmHg when the loop of Henle of the index nephron was perfused and from 0.7 +/- 0.3 to 12.3 +/- 1.0 mmHg when the loop of an adjacent nephron was perfused. At reduced arterial blood pressure (82.8 +/- 1. 3 mmHg), index nephron perfusion with CHA increased PSF responses from 4.5 +/- 0.3 to 9.4 +/- 0.4 mmHg. In AT1A -/- mice with a mean arterial blood pressure of 80 +/- 1.9 mmHg, CHA increased PSF responses only from 0.1 +/- 0.3 to 3.6 +/- 0.54 mmHg during index nephron perfusion and from 0.25 +/- 0.2 to 2.7 +/- 0.55 mmHg during adjacent nephron perfusion, significantly less than in wild-type animals (P < 0.001). Responses to CHA were intermediate in AT1A +/- mice. Thus AT1A receptor knockout mice show a markedly reduced constrictor response to CHA both in the presence and absence of simultaneous activation of the tubuloglomerular feedback system. These data support the notion of a functional interaction between adenosine and angiotensin II in the regulation of afferent arteriolar tone.

Adenosine↗

PubMed1996

Contrast perception across changes in luminance and spatial frequency.

The interaction of the effects of luminance and spatial frequency on perception of suprathreshold contrast was studied with use of a contrast-matching paradigm. Four subjects matched the appearance of Gabor patches at different luminances and spatial frequencies. The contrast of a 1-octave Gabor test patch at one of five frequencies [1-16 cycles/degree (c/deg) in 1-octave steps] and at one of seven mean luminance levels (0.5-50 cd/m2 in 1/3-log-unit steps) was matched, by the method of adjustment to a standard patch of 3 c/deg at 50 cd/m2 at a nominal contrast of 0.3. For each block of trials the spatial frequency of the test patch was randomly changed (three repetitions at each frequency per block) while the luminance was fixed. The subject regularly shifted fixation between the two targets in response to a metronome tone every 1.5 s. Contrast constancy was demonstrated across the entire luminance range tested for all but the two highest frequencies. For 8 c/deg the perceived test contrast was reduced only when the luminance was less than 2 cd/m2. For 16 c/deg, perceived contrast decreased linearly (with a slope of -1/2 on a log scale) with decreases in luminance across the entire luminance range. As at threshold, reduction in luminance across the levels commonly available on a CRT display has only minimal effects on low-frequency suprathreshold contrast perception. However, the apparent contrast of high-frequency features, in binocular free-viewing conditions, is rapidly reduced with a local reduction in screen luminance. This effect has important implications for visual models used in image-quality analysis.

Adaptation, Physiological↗

PubMed1995

The critical role of relative luminance relations in White's effect and grating induction.

It has been proposed that both White's effect and the grating induction effect are examples of brightness induction phenomena modeled in terms of local spatial filters. We have shown that for these illusions to occur it is necessary that the luminance of the gray target elements falls between that of the inducing stripes of the square-wave pattern. This critical role of luminance relationships is not predicted by existing models of these illusions.

Color Perception↗

PubMed1992

Color changes in red and white projections at low luminance.

In previous studies photographic projections containing only mixtures of red and white light produced a wide variety of hues. In the present experiment similar patterns were produced on a color monitor. Squares with four combinations of red and white light were studied at two mean luminances. Subjects reported that a chromaticity that appeared pink at our higher luminance appeared yellow at low luminance.

Color Perception↗

PubMed1990

Perceived lightness, but not brightness, of achromatic surfaces depends on perceived depth information.

Three experiments were conducted in an attempt to replicate and clarify Gilchrist's (1977, 1980) experiments on the effects of depth information on judgments of achromatic surface color. Gilchrist found that coplanarity, and not retinal adjacency, was the dominant factor in determining achromatic color matches. Because such matches can be made on the basis of either brightness or lightness, we obtained judgments of both qualities. Stereopsis was added to enhance the perceived depth effect of Gilchrist's display, which was otherwise simulated closely on a high-resolution CRT. The results for lightness followed the same pattern as those of Gilchrist, but were smaller in magnitude. This discrepancy may reflect reduced extraneous lighting effects in our displays. Our results therefore agree with related studies in suggesting that lightness matches are based on relationships among coplanar surfaces. Brightness matches, however, were not influenced by perceived depth.

Adult↗

PubMed1986

Simultaneous color constancy.

Observers matched patches (simulated Munsell papers) in two simultaneously presented computer-controlled displays, a standard array presented under 6500-K illumination and a test array under 4000 or 10,000 K. Adaptation to the test illuminants was limited. The adjusted patch was surrounded by a single color (annulus display) or by many colors (Mondrian display). Observers either matched hue and saturation or made surface-color (paper) matches in which the subject was asked to make the test patch look as if it were cut from the same piece of paper as the standard patch. For two of the three subjects, the paper matches were approximately color constant. The hue-saturation matches showed little color constancy. Moreover, the illumination difference between the two displays was always visible. Our data show that simultaneous mechanisms alone (e.g., simultaneous color contrast) alter hues and saturations too little to produce hue constancy.

Color↗