VIRUS ISOLATION ATTEMPTS FROM URINE OF PATIENTS WITH RENAL DISEASE.
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Biomedical subjects
Publications and source records attributed to L B GUZE.
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Bacteria may persist in "protoplast" form in kidneys after treatment of experimental enterococcal pyelonephritis with penicillin. Erythromycin, while ineffective against the bacterial form of infection, was able to kill "protoplasts" in vivo. This finding is consistent with the hypothesis that intact cell wall interferes with the ingress of erythromycin into the cellular area in which it acts.
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Hubert, Earl G. (Veterans Administration Center, Los Angeles, Calif.), George M. Kalmanson, and Lucien B. Guze. Preservation of antigen-coated sheep erythrocytes by freezing for use in indirect hemagglutination procedure. J. Bacteriol. 86:569-572. 1963.-Preservation of sheep erythrocytes, coated with various protein and lipopolysaccharide antigens, was accomplished by rapid freezing and storage at -70 C in a dextrose-lactose solution. The stored cells were tested at intervals in an indirect hemagglutination procedure. Sensitivity of these cells was maintained, and antibody titers were consistently reproducible throughout the period of testing. These observations suggest that sheep erythrocytes may be coated with a variety of antigens and may be employed satisfactorily in an indirect hemagglutination test, even after storage at -70 C for a period up to 6 months.
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