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L B Koutny

Publications and source records attributed to L B Koutny.

4 recordsLinked to original sources
PubMed1997

Immunoassay for thyroxine (T4) in serum using capillary electrophoresis and micromachined devices.

As part of our ongoing effort to develop electrophoretic assay technology for clinical diagnostics, we describe a competitive immunoassay for the determination of serum thyroxine (T4) based on electrophoresis and laser induced fluorescence (LIF). Measurements of total T4 are useful for the clinical evaluation of thyroid function. A fluorescein thyroxine conjugate was utilized in conjunction with a polyclonal antibody preparation as assay reagents. Capillary electrophoresis (CE) conditions tolerant of the direct injection of serum without extraction or other sample preparation steps were developed and used for quantitation of total T4 in serum. We have been exploring the use of micromachined devices with arrays of channels for high assay throughput. Our assay protocol was carried in a microchip format. The results illustrate that gains in speed can be additionally achieved, with the electrophoretic separation of free from bound labelled T4 being performed in about 15 s for serum samples.

Electrophoresis, Capillary↗
PubMed1996

Microchip electrophoretic immunoassay for serum cortisol.

An immunoassay performed using a microchip electrophoretic system is described. Separation and quantitation of free and bound labeled antigen in a competitive assay are carried out in channels micromachined into fused silica substrates. Such microchips are attractive because of their small size, ruggedness, and amenability to automated handling. The assay achieves the determination of cortisol in blood serum over the range of clinical interest (1-60 micrograms/dL) without the need for extraction or other sample preparation steps. The separation is performed in less than 30 s. Very high throughput is possible by operating the assay in multiple channels in parallel. These characteristics make microchip electrophoretic systems a promising technology for the rapid analysis of clinical samples.

Electrophoresis↗
PubMed1993

Expert system for data acquisition to achieve a constant signal-to-noise ratio: application to imaging of DNA sequencing gels.

Normally, data acquisition and processing in chemical measurements are based on equal time (effort) for each data point. This leads to uneven signal-to-noise ratios (S/N) for each point. We present an expert system which decides in real time whether adequate S/N has been achieved for each point. If so, that data point will be omitted in all further acquisition or processing operations, greatly increasing the overall efficiency. The special case of analyzing DNA sequencing gels is tested with this scheme. A factor of 7.6 reduction in effort is demonstrated for a data set of 384 x 250 pixels, which normally would require 6 million sequential operations (64 frames).

Base Sequence↗