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Biomedical subjects

L B Nilsson

Publications and source records attributed to L B Nilsson.

25 records · Page 2Linked to original sources
PubMed1990

Determination of remoxipride in plasma and urine by reversed-phase column liquid chromatography.

A reversed-phase column liquid chromatographic method for the determination of remoxipride, a novel antipsychotic drug, in biological fluids is described. A simple one-step extraction is used followed by liquid chromatography on a 3-microns octadecylsilica column and ultraviolet absorbance detection. The method is accurate and precise for clinical remoxipride levels in both plasma and urine. For situations where a higher sensitivity is necessary a two-step extraction and a modified mobile phase are used. With this modification plasma concentrations down to 2 nM can be determined with acceptable precision.

Antipsychotic Agents↗
PubMed1988

Reversed-phase ion-pair liquid chromatographic method for the determination of low concentrations of haloperidol in plasma.

A sensitive liquid chromatographic method for the determination of haloperidol in plasma is described. The efficient and simple extraction procedure, followed by reversed-phase ion-pair liquid chromatography on a 3-micron octadecylsilica column and UV absorbance detection, makes it possible to determine concentrations down to 0.5 nmol/l with acceptable precision. In a pharmacokinetic study, in which 5 mg of haloperidol were given orally, the plasma levels were followed for 48 h.

Chromatography, Liquid↗
PubMed1982

Long-term, low-dose treatment with pivmecillinam alone and in combination with pivampicillin in patients prone to recurrent bacteriuria.

The results of three separate studies aimed at evaluating the efficacy of long-term, low-dose treatment with pivmecillinam alone, and in combination with pivampicillin, in patients prone to recurrent bacteriuria, are presented. Overall, pivmecillinam and pivmecillinam/pivampicillin appeared to be equally effective, both clinically and bacteriologically, in a group of elderly subjects treated for 6 months. Pivmecillinam was also used successfully for up to 1 year in 10 out of 12 children in whom prophylactic therapy was indicated. The various regimens employed were without any significant toxic effects and were well tolerated.

Aged↗
PubMed1980

Simultaneous determination of dopamine, DOPAC and homovanillic acid. Direct injection of supernatants from brain tissue homogenates in a liquid chromatography--electrochemical detection system.

A simple method based on high-performance liquid column chromatography with electro-chemical detection is described for the simultaneous determination of dopamine, 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) in discrete brain regions of rats. The supernatant of a tissue homogenate is injected directly onto a liquid chromatograph, thus omitting the commonly adopted adsorption step. Of the four different supports tested Nucleosil C15 (5 micron) was found superior with respect to chromatographic performance. The effects of pH, methanol and the ion-pairing agent hexyl sulfate on the retention were studied. The mobile phase used in the final studies consisted of citrate buffer pH 4.25-methanol (92:8, v/v) containing hexyl sulfate (1.7 . 10(-3) M). Standard curves of dopamine, DOPAC and HVA were found linear up to about 600 pmol per injection for each compound. The precisions of the chromatographic step were (Srel. %): 0.72% (dopamine), 1.26% (DOPAC) and 2.69% (HVA).

3,4-Dihydroxyphenylacetic Acid↗
PubMed1976

Treatment of chronic urinary tract infections with cephalexin (Ceporexine) orally administered at 12-hourly intervals.

19 patients with chronic urinary tract infections were treated, after culture and sensitivity testing, with cephalexin (Ceporexine) 1 g twice daily for 10 days. All patients were free from bacteria after 2 days treatment and 18 after the treatment was finished. After 30 days, 7 had a recurrence of the urinary tract infection. The result shows that cephalexin given twice a day in the dosage of 1 g has as good an effect in the treatment of urinary tract infections as when the dosage is given 4 times a day.

Aged↗
PubMedDate not supplied

High sensitivity determination of the remoxipride hydroquinone metabolite NCQ-344 in plasma by coupled column reversed-phase liquid chromatography and electrochemical detection.

An HPLC method for the determination of NCQ-344, a remoxipride metabolite with a hydroquinone structure, in human plasma is described. Special precautions for the sampling were necessary as the compound rapidly decomposes. An efficient clean-up of the plasma samples was necessary to make use of the inherent sensitivity of the electrochemical detector. This was accomplished by a fast and simple liquid-liquid extraction at pH 7.05 combined with further cleaning on-line by using a short cyanopropyl column as the first column in a column switching system. A heart-cut from the cyanopropyl column containing the NCQ-344 fraction was then injected onto the analytical octadecyl silica column and NCQ-344 was detected at an oxidation potential of 0.70 V. The absolute recovery was > 95% and concentrations down to 0.10 nM could be determined with acceptable precision. The NCQ-344 levels in a limited number of samples from patients given remoxipride were found to be between 0.10 and 1 nM. The remoxipride concentrations in the same samples were 5,000-20,000 nM.

Chromatography, High Pressure Liquid↗