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Biomedical subjects

L Baldi

Publications and source records attributed to L Baldi.

26 records · Page 2Linked to original sources
PubMed1989

Genotypic analysis of engraftment in thalassemia following bone marrow transplantation using synthetic oligonucleotides.

DNA hybridization with synthetic oligonucleotide probes was used to assess engraftment in 19 thalassemic patients who received bone marrow grafts from their respective healthy HLA-identical siblings. Three oligomers complementary to the tandem repetitive sequences of different hypervariable regions of human DNA were designed so as to produce simple RFLP (restriction fragment length polymorphism) patterns. Each probe hybridizes to one or two bands in HinfI-digested genomic DNA. The combined use of these three probes allowed a discrimination between all the HLA-identical siblings tested. Both donor-specific and recipient-specific DNA fragments existed in 18 out of the 19 sibling pairs studied. One pair possessed only a donor-specific fragment. DNA analysis at an early stage after the graft detected donor-specific fragments in 15 out of 19 patients, recipient-specific fragments in three patients and a mix of recipient and donor fragments in one patient. At a later stage this patient possessed donor-specific fragments only. Follow-up DNA analysis confirmed these findings. Thus 16 patients continued to display donor-specific fragments over 60 days post-transplant. These DNA data showed strong correlation with the clinical status of the patients as well as with other markers of engraftment including cytogenetics and hemoglobin synthesis. The patients who showed donor-specific fragments over 60 days have been free of thalassemic symptoms for over 300 days. Moreover, in 11 cases it was possible to predict the fate of the graft within 15 days after transplantation. In conclusion, the use of the three synthetic oligonucleotide probes provides a powerful tool in documenting engraftment in bone marrow transplantation.

Adolescent↗
PubMed1987

[Prevalence and occurrence of infections caused by hepatitis B virus in the dialysis units in the Apulia region].

Sera of 803 hemodialysis patients and 413 staff members were tested to evaluate the relationship between infectivity markers and spread of HBV infection in dialysis units. HBsAg was detected in 13.8% patients undergoing chronic hemodialysis and 3.9% staff members. High prevalence of HBeAg and DNA polymerase activity was observed only in HBsAg positive patients. The highest titers of HBsAg and anti-HBc were detected in hemodialysis patients, whereas asymptomatic carriers showed low titers of these markers. A highly significant correlation was recorded between detection of HBeAg in patients and presence of serum DNA activity. These data suggest that in HBsAg hemodialysis patients a more active viral replication occurs and a higher contagiousness of these subjects.

Adolescent↗
PubMed1985

Androgen levels following a football match.

In 18 trained football players, blood samples were collected before a football match, at half-time, at the end, and 45 and 90 min after the end of the match. The testosterone (T) level showed a decrease in the rest period. Dihydrotestosterone (DHT) increased during the match, but returned to initial levels in the last samples. The T/DHT ratio decreased, reached the minimal value at the end of the exercise, and returned to basal levels in the rest period. Cortisol and androstenedione levels increased during the match, but returned to control levels 45 and 90 min after the end of the match, respectively. It is suggested that during this type of exercise anabolic and catabolic hormones may be simultaneously activated.

Adult↗
PubMed1983

[Changes in laboratory and clinical parameters in subjects with chronic brucellosis treated with an immunomodulator: methisoprinol].

In order to evaluate the immunomodulating activity of isoprinosine in chronic infections, 7 patients with chronic brucellosis unresponsive to antibiotic and corticosteroid therapy were treated with 4 g/die of isoprinosine in association with antibiotic therapy from 3 to 6 months. Immunoserologic and clinical evaluation were obtained at regular intervals from each patient. The treatment induced a significant increase of E rosette formation, reduction of erythrocyte sedimentation rate, associated with improvement or disappearance of clinical symptoms. These data suggest that isoprinosine may favourably influence the clinical course of chronic brucellosis, probably modifying host cell-mediated immune response.

Anti-Bacterial Agents↗
PubMed1982

[Comparative evaluation of 5 third-generation methods for the detection of HBsAg].

Five third generation methods for detection of HBsAg in serum were evaluation: AUSRIA II, Micro-RIA, Hepanosticon, Enzygnost, Antigen-TG. Sera of 340 patients with acute and chronic hepatitis and 160 controls were examined with these techniques. The haemoagglutination and latex tests were less sensitive than the RIA, Micro-RIA and ELISA methods. The RIA procedure remains the more sensitive, as demonstrated with sera diluted. False positive results, frequently depending on the excess of antibodies, were observed in ELISA, haemoagglutination and latex tests. The results confirm that the RIA procedure is still highly specific and sensitive, but ELISA is also a very alternative method for HBsAg detection.

Agglutination Tests↗
PubMed1981

[Sensitivity and specificity of 2 methods of determining surface antigen (HBsAg) in various forms of hepatitis B pathology].

A passive haemoagglutination method (rHA) was compared to a solid-phase radioimmunoassay (RIA) in detecting hepatitis B surface antigen (HBsAg) in order to evaluate their sensitivity and specificity. The test was performed on sera from 297 subjects with acute and chronic hepatitis, 23 asymptomatic HBsAg-RIA positive carriers, 20 patients with infectious mononucleosis, 110 HBsAg RIA negative healthy persons; 30 sera positive for rheumatoid factor and/or autoantibody were also tested. Our data confirm that RIA is highly specific and rarely shows false negative results, depending on antibody excess, rHA shows less sensitivity than RIA in detecting HBsAg especially in sera of patients with acute hepatitis.

Carrier State↗
PubMedDate not supplied

A non-hormonal therapeutic alternative in cervico-vaginal dystrophies.

The pharmaco therapeutic effect of polydeoxyribonucleotide vaginal suppositories in two dosages (PDRN 1,65 mg and PDRN 5 mg)* on vaginal dystrophy was studied. 40 women, aged more than forty, in surgical or physiological climacteric with cervico-vaginal dystrophy were examined. The study was carried out according to the double-blind method, randomized, between groups. Both treatments produced important ameliorations of subjective symptomatology and objective signs. No significant difference emerged between the two preparations. PDRN was proved to be a valid alternative to local hormonal therapies.

Administration, Topical↗