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L Banek

Publications and source records attributed to L Banek.

5 recordsLinked to original sources

[Development of peritubular myoid cells in the rat testis].

In the testes of 47 embryos, fetuses and newborn rats (Fisher strain), until the 15th day of life, the appearance and numerical dynamics of "premyoid" and myoid cells were analysed by light microscopy. The material was prepared routinely (fixed in Bouin fluid, embedded in paraffin, and cut to the thickness of 7 micrometers). The embryos of the 12th to 17th day of gestation were serially sectioned in toto, while fetal testes on the 18th to 21th day of gestation were removed and serially cut as well as the rat testes after birth. In each testis ten seminiferous tubules (cords) sectioned perpendicularly were analysed and only in two testes was it possible to find out nine but not ten perpendicularly sectioned tubules. Until the first week after birth, the lengthened cells that envelope tubules (cords) are called "premyoid". In the second half of the second week two types of lengthened cells in the envelope could be seen: one with a very thin, dark nucleus and the other with a lighter and thicker nucleus located peripherally, toward the interstitium. The former are considered myoid cells and the latter as fibroblasts. The numerical dynamics of "premyoid" and myoid cells evidently depends on the "pulsatile" effect of gondotropins. In the prenatal period, it was possible to see two increases in the number of these cells: on the 16th and on the 20th day.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals

[Sertoli cell secretion].

The article deals with the secretion of Sertoli cells. Different substances they secrete and their role in the testis are described. These cells release a number of factors in their environment, thus creating a local "milieu" and exerting a local control of spermatogenesis in the seminiferous tubules. As target cells for FSH and testosterone, Sertoli cells are also included in a systemic control of spermatogenesis, and because of that, they are necessary for the normal function of the male sex gland.

Animals

[Gonocytes in the fetal and early postnatal period (stereologic study)].

The behaviour of gonocytes from the 15th prenatal to the 6th postnatal day was studied in testicles of 32 Wistar rats. Histological and cytological methods were applied. Cytological smears were obtained by the imprint method. It was established that the number of gonocytes per cubic millimeter of the tissue for the postnatal group of rat testicles was seven times smaller in respect to the postnatal group. It was 102,338 +/- 15,903 mm0/mm3 for the prenatal group and 13,783 +/- 2538 mm0/mm3 for the postnatal group. The number of gonocytes in the first investigated prenatal day (the fifteenth day) in comparison with the last postnatal day (the sixth day) was even thirty times smaller (i.e. 157,800 against 4,900 mm0/mm3). This work is the first to give morphologic and quantitative data on gonocytes in cytologic smears.

Animals