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L Barclay

Publications and source records attributed to L Barclay.

5 recordsLinked to original sources
PubMed1992

A comparison of the frequency and type of chromosomal abnormalities in human sperm after different sperm capacitation conditions.

Human sperm karyotypes can be prepared after fusion of human sperm with Golden hamster oocytes. Most laboratories use one of two methods of sperm capacitation: incubation of freshly-ejaculated sperm in Biggers, Whitten, and Whittingham (BWW) medium for 5-7 h at 37 degrees C or sperm storage in (N-tris [hydroxymethyl]methyl-2-aminoethanesulfonic acid; 2-([2-hydroxy-1,1-bis(hydroxymethyl)ethyl]amino)ethanesulfonic acid) (TES)-Tris yolk buffer (TYB) for 1-3 days at 4 degrees C. Since there have been conflicting reports as to whether there is a difference in the frequency of structural chromosomal abnormalities between BWW capacitation and storage in TYB for 2 days, we analyzed a larger number of karyotypes (8974) from 136 donors to determine if there was any difference in the frequency or type of chromosomal abnormalities in sperm treated by fresh BWW capacitation, storage in TYB for 1 day (TYB-1), or storage in TYB for 2 days (TYB-2). There was no difference in the frequency of numerical chromosomal abnormalities or sex ratio in any of the three treatment groups. However, there was a significantly increased frequency of structural chromosomal abnormalities after storage in TYB-1 and TYB-2. There was no difference in the frequency or type of structural chromosomal abnormalities after sperm storage in TYB-1 compared to TYB-2.

Adult↗
PubMed1991

Relatives' recall of a psychiatric interview.

One hundred seventeen patients from the Burke Rehabilitation Center (White Plains, NY) with clinically diagnosed dementia of the Alzheimer type (DAT) were identified for a case-control study (CCS) designed to assess risk factors for DAT. A psychiatric evaluation was part of the Burke dementia workup. The study sample was composed of 96 relatives who (a) were present with the patient at the psychiatric evaluation and (b) were interviewed for the CCS. The data for this study were the relatives' responses to the question "Did your relative (patient with DAT) ever see a psychiatrist?" The recall of relatives, regardless of relationship category (spouse, adult children, and others) did not exceed the 50% recall predicted by chance. When the age of the relative and length of time between the two interviews were considered, they did not modify these results.

Adult↗
PubMedDate not supplied

Development of an instrument to study the sexual relationship of partners during pregnancy.

The development of an objective self-report instrument designed to identify the domain of sexual behaviours during pregnancy is described. The Pregnancy and Sexuality Questionnaire (PSQ) is concerned with sexual interest and arousal and with specific sexual behaviours of partners in a stable heterosexual relationship. Data were obtained two weeks apart from 15 couples presenting to an antenatal class and from two additional females whose partners did not participate. The validity and reliability of the PSQ are considered with particular emphasis on test-retest reliability. The results in general support the reliability of the instrument. The questionable reliability of some items is attributed to the natural sequence of change in sexual behaviours during pregnancy and to the relatively small sample size used. The PSQ appears to be sufficiently robust to be confidently applied both in sexual behaviour research and as an adjunct to clinical practice.

Adult↗