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L Burgoyne

Publications and source records attributed to L Burgoyne.

6 recordsLinked to original sources

Laryngeal mask vs intubating laryngeal mask: insertion and ventilation by inexperienced resuscitators.

The laryngeal mask airway (LMA) has been shown to be useful in airway maintenance during resuscitation. The intubating laryngeal mask (ILM) is a modified LMA permitting both ventilation and rapid endotracheal intubation. We aimed to compare the LMA and the ILM with regard to ease of insertion and successful ventilation by inexperienced personnel. We have used anaesthetized, apnoeic, non-paralysed patients as a model to simulate resuscitation. Following standardized training, non-anaesthetic medical staff with no previous experience in laryngeal mask airway insertion (novices) inserted either the LMA or ILM in 55 patients following induction of anaesthesia. There were no differences between the two patient groups included in our study with regard to mean age and body mass index (BMI). The success rate for inserting the airway device and achieving a significant end-tidal CO2 recording within two minutes was 23/28 for the LMA (82.1%) and 22/27 for the ILM (81.5%). Reasons for failure included inability to insert the ILM past the teeth and insertion of the LMA upside down. There were no clinically relevant differences in the mean time to airway insertion and successful ventilation (62.6 vs 62 seconds) or expired tidal volume (781 vs 767 ml) for the LMA and ILM respectively. We conclude that the ILM is as easily inserted and effectively used as an LMA by novices and, because it allows the option offacilitating endotracheal intubation, may be the preferred device for maintaining an airway during resuscitation.

Adult↗

Bacterial typing: storing and processing of stabilized reference bacteria for polymerase chain reaction without preparing DNA--an example of an automatable procedure.

This paper examines the use of bacteria killed on blood-storage paper as templates (ghosts) and takes, as an example, the PCR ribotyping (amplification of the intergenic spacer regions between the 16S and 23S ribosomal RNA genes) of bacteria as described by Kostman et al. (J. Infect. Dis. 171, 204-208, 1995). All procedures have been particularly designed to be compatible with automation. DNA preparation is inappropriate for routine, high-volume sequence amplification from a diversity of microorganism cultures. Blood-storage/processing media provide another way of processing samples for PCR with distinctive aspects of increased safety and ease of automatibility. Blood-storage paper can be used for killing and processing bacteria to DNA-containing ghosts for reliable PCR. From as little as a few microliters of an overnight culture or a reasonably sized, single colony, rapid processing of large sample numbers is possible. FTA blood-storage medium has additional utility in that long-term cataloguing, storage, and processing of paper, loaded with culture, for PCR, is possible via a variety of sample wash sequences. It can be performed at convenience, after collection, and can be delayed indefinitely. using this approach, the repeatability of some PCR-ribotyping methodology of the type used by Kostman et al. (J. Clin. Microbiol. 30, 2084-2087, 1992) was examined as an exercise to demonstrate the practicality of FTA blood-paper usage and to check some basic features of PCR ribotyping. Five strains of Staphylococcus and one strain Escherichia coli were stored and processed on FTA blood paper, the PCR-ribotype patterns were analyzed and found to be the equal of patterns previously seen via additional DNA preparations.

Bacteria↗

Human satellite-III DNA: an example of a "macrosatellite" polymorphism.

Human satellite III DNA contains a complex polymorphism, which appears to be TaqI-specific. Its likely cause is a two-step point mutation in the pentameric repeat TTCCA, typical of satellite III. Hybridization of the satellite-III sequence-related probe that demonstrates this polymorphism is directly attributable to clusters of "pure" pentameric TTCCA repeats in the genome. The sites of such repeats include the 3.4-kb fragment specific to the Y chromosome and a limited number of autosomes. The polymorphism arises from the latter and is likely to include chromosomes containing so-called K domain satellite III sequences found, for example, in chromosomes 9 and 15. Segregation of the polymorphic fragments appears to follow orthodox genetics.

Animals↗