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Biomedical subjects

L Canales

Publications and source records attributed to L Canales.

At least 19 recordsLinked to original sources
PubMed2003

Effect of neuropeptide Y on GnRH-induced LH release from bovine anterior pituitary cell cultures derived from heifers in a follicular, luteal or ovariectomized state.

Objectives were to determine if neuropeptide Y (NPY) had direct effects GnRH induced secretion of LH from the anterior pituitary gland, and if endogenous steroids modulated the effect of NPY. To accomplish these objectives, 15 Hereford heifers were assigned to one of three ovarian status groups: follicular, luteal, or ovariectomized. One animal from each of the three ovarian status groups was slaughtered on each of 5 days and anterior pituitary gland harvested. Anterior pituitary gland cells within ovarian status were equally distributed and randomly assigned to one of three cell culture treatments: no NPY or GnRH (control), 10 nM GnRH, or 100 nM NPY+10 nM GnRH. Anterior pituitary cell cultures were incubated with or without NPY for 4 h and further incubated for an additional 2 h with or without GnRH and supernatant collected for quantification of LH. Treatment of anterior pituitary cell cultures with GnRH or GnRH+NPY did not affect LH release in cultures obtained from follicular (S.E.=5%; P=0.58) or ovariectomized (S.E.=7%; P=0.22) heifers. Both GnRH and GnRH+NPY increased LH release from anterior pituitary cell cultures from heifers in the luteal phase (S.E.=14%; P < or = 0.05) compared to control cultures. Cultures from luteal phase heifers treated with GnRH did not differ from those treated with GnRH+NPY (P=0.34). These data provide evidence to suggest that effects of NPY on LH release may occur primarily at the level of the hypothalamus.

Animals↗
PubMed1995

[Disseminated nocardiosis in 3 immunocompetent patients].

Nocardia asteroide infections, an aerobic actinomycete, have several forms and lungs, skin and brain are the organs most frequently involved. When the infection suspected, special staining methods must be ordered to identify the agent. We report three immunocompetent patients with disseminated nocardiosis, two presenting with nodular lesions of skin and lungs and one presenting with pulmonary involvement and brain abscesses. The importance of clinical suspicion and early diagnosis of nocardiosis is emphasized.

Adolescent↗
PubMed1992

Metastatic tumors to the upper gastrointestinal tract: endoscopic experience.

Metastatic tumors to the upper gastrointestinal tract were identified by esophagogastroduodenoscopy in 14 patients. Malignant melanoma, breast cancer, and lung cancer were the most common primary cancers in four, three, and three patients, respectively. Osteogenic sarcoma, renal cell carcinoma, Meckel cell carcinoma of the skin, and germ-cell tumor were the primary cancer in the remaining four. The esophagus was involved in three patients, the stomach in 13, duodenum in four, and papilla of Vater in one. Upper gastrointestinal bleeding and anemia were the most common presenting features. There was correlation between symptoms and endoscopic findings in all patients. Involvement of gastrointestinal tract at endoscopy was the initial and only evidence of metastases in all patients without evidence of metastases elsewhere, as evidenced by other diagnostic tests in any of these patients. Endoscopic biopsies and/or brush cytology provided histologic diagnosis in all 14 patients. The endoscopic and nonendoscopic literature regarding metastases to the upper gastrointestinal tract is reviewed.

Adenocarcinoma↗
PubMed1990

Suppression of T-lymphocyte responses to Entamoeba histolytica antigen by immune sera.

Lymphocytes from patients cured of amebic liver abscesses proliferate and produce gamma interferon upon incubation with soluble Entamoeba histolytica antigen: however, amebic liver abscesses exhibit a relentless progression without treatment. To determine whether suppressive factors are present in sera, we studied T-lymphocyte responses to total soluble E. histolytica antigen by using cells from five patients treated for amebic liver abscesses in the presence of 15 different immune sera and 10 control sera. In the presence of immune sera, E. histolytica antigen-induced lymphocyte proliferation decreased by 63% and production of gamma interferon was reduced by 93.2% (P less than 0.01). Immune sera had no effect on the mitogenic responses of patient lymphocytes to phytohemagglutinin or on the proliferative responses of control lymphocytes to phytohemagglutinin or tetanus toxoid. The suppressive activity of immune sera diminished as the time between therapy for amebic liver abscesses and serum collection increased (P less than 0.05). Suppressive activity did not correlate with the titers of serum anti-amebic antibody and was not affected when serum was absorbed with viable amebic trophozoites. In conclusion, soluble factors present in the sera of amebic liver abscess patients suppressed in vitro lymphocyte responses to E. histolytica antigen and may have contributed to the lack of development of effective in vivo cell-mediated immune responses following the onset of amebic liver abscesses.

Animals↗
PubMed1983

Algorithm-directed care by nonphysician practitioners in a pediatric population: Part I. Adherence to algorithm logic and reproducibility of nonphysician practitioner data-gathering behavior.

The ability of quickly trained nonphysician practitioners to care for pediatric patients with upper respiratory tract infections (URIs) was evaluated in 3802 patients. These nonphysician practitioners (Army Pamosists) used an explicit treatment protocol and computerized audit of protocol adherence. Pamosists omitted protocol-suggested plans in 3.7 per cent of cases and ordered unnecessary treatment plans in 1.7 per cent of cases. They did not obtain a suggested MD consultation in 6.2 per cent of the cases. Agreement between Pamosists and pediatricians on data and management decisions (PM-MD study) was then compared with agreement on the same variables between pairs of a group of five pediatricians who saw a separate but comparable group of 103 pediatric URI patients (MD-MD study). The Pamosists demonstrated good (77 to 89 per cent) overall agreement with pediatricians, and the amounts of agreement between Pamosists and pediatricians in the PM-MD study did not generally differ significantly (p greater than 0.05) from the agreement on the same variables between pairs of pediatricians in the MD-MD study.

Allied Health Personnel↗
PubMed1981

Algorithm-directed triage in a pediatric acute care facility: a retrospective study.

Algorithm-directed triage by nonprofessionals was used to safely assign care urgency categories to 22,934 walk-in patients under 13 years of age during 1978. Of all patients, 16.7% were categorized as having urgent or potentially urgent problems at triage. Of those patients admitted, 79.12% received these urgency classifications. Additionally, 72.13% of all patients received an acute minor illness care urgency classification. Of patients admitted, 20.87% had an acute minor illness classification. A total of 11.16% of all patients received a routine or non-urgent classification. No patients admitted had been triaged to this non-urgent classification. As determined by review of records of 91 patients admitted from the acute care facility, the system safely identifies both high- and low-risk walk-in populations.

Acute Disease↗
PubMed1980

Algorithm-directed triage of pediatric patients. A prospective study.

An Army corpsman used physician-written triage algorithms to rate the urgency of the chief complaints of 2,000 pediatric outpatients. His ratings agreed with subsequent ratings by physicians in 84% of cases. The corpsman assigned a higher care urgency classification in 15% of cases and a lower classification in only 1.2% of cases. No danger to patients resulted from the algorithm-directed screening. Use of a "nonprofessional" as a triage agent spares the pediatrician, pediatric nurse practitioner, and nurse for providing health care. With increasing use of acute care facilities by patients without appointments, physician-written algorithms allow triage agents who lack formal medical training to determine safely the need for care of patients.

Allied Health Personnel↗
PubMed1977

Reversible paralysis of automatic respiration in multiple sclerosis.

On a background of acute bulbar dysfunction, a patient experienced the simultaneous onset of severe proprioceptive loss and paralysis of automatic respiration that rapidly reverted to normal. Subsequent progressive neurological deterioration over several months ended in death, and neuropathological examination revealed demyelination in discrete distribution. The anatomical and diagnostic implications of this are briefly discussed.

Adult↗
PubMed1976

Screening for cell-mediated immunity in children.

Skin testing and in vitro lymphocyte blastogenesis with Monilia and tetanus toxoid were examined as methods of screening cell-mediated immunity (CMI) in infants and children. With increasing age, all assays were more likely to be positive. Tetanus toxoid was a better single screening antigen than Monilia, particularly for young infants. Combining all assays, at least one was positive in 88% of children over 6 weeks of age. Combining the two skin tests, at least one was positive in 73% of children in the same age range. Results for the blastogenic assays indicated that this in vitro test is more sensitive than the in vivo skin test for demonstrating CMI to these two antigens. Infants with documented histories for Monilia diaper rash had a significantly higher probability of having a positive skin or blastogenic reaction with Monilia.

Age Factors↗
PubMed1975

Cellular immune responses to Herpes simplex virus type 1 in recurrent herpes labialis: in vitro blastogenesis and cytotoxicity to infected cell line.

Cellular immunity to herpes simplex virus type 1 (HSV-1) in 12 volunteers with recurrent herpes labialis was evaluated by means of two microassays. In the blastogenesis assay, lymphocytes were incubated with tissue culture cells persistently infected with HSV-1. Uninfected cells were used as controls, and a blastogenic index was calculated. The mean blastogenic index (plus or minus SD) for subjects with recurrent herpes labialis was 26.9 (plus or minus 8.3); the mean blastogenic index (plus or minus SD) in control donors with antibody to HSV-1 was 13.4 (plus or minus 7.2). The difference between these values was statistically significant (t equals 4.154; P smaller than 0.001). In the cytotoxicity assay, cells of the same persistently infected line were used as target cells, and release of 51-Cr from these cells or from control cells served as the index of lymphocyte reactivity. Specific immune release attributable to HSV-1 averages 3.7% (plus or minus 1.8%) in subjects with recurrent herpes labialis, compared with 23.1% (plus or minus 9.8%) in controls (t equals 6.135; P smaller than 0.001). These data suggest a dissociation between mechanisms of cellular immunity, with enhanced lymphocyte blastogenesis but decreased cytotoxicity. Recurrent herpes labialis may thus result from subtle cellular immune deficiency involving at least one of the efferent mechanisms.

Adenoma↗