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Biomedical subjects

L Cerva

Publications and source records attributed to L Cerva.

At least 37 records · Page 2Linked to original sources
PubMed1980

Naegleria fowleri: trimethoprim sensitivity.

Trimethoprim in a concentration of 4 micrograms per milliter of Bacto-Casitone (Difco) medium inhibits the growth of nonvirulent Naegleria fowleri isolates. The growth of virulent strains is unaffected even with 400 micrograms of the drug per milliliter of medium. Differences in sensitivity constitute the possibility of a simple selection of environmental isolates. The pathogenicity and virulence of Naegleria species may be connected with the metabolism of folic acid.

Amoeba↗
PubMed1980

Virulent Naegleria fowleri in indoor swimming pool.

Naegleria fowleri was isolated from water during a hygienic inspection of a swimming pool in December 1977. This swimming pool was identified as a source of the infectious agent in the years 1962-1965, when a large outbreak of primary amoebic meningoencephalitis (PAME) occurred. First two strains of N. fowleri, pathogenic for white mice after intracerebral and intranasal inoculation, were isolated from water of outlet troughs, additional strains were then isolated from various places; particularly from a cavity in the damaged wall of the pool. The incubation temperature did not inhibit a simultaneous growth of amoebae of the genera Acanthamoeba, Flabellula, Hartmannella and Vahlkampfia in the primocultures. Epidemiological investigations did not reveal any new case of PAME in relation with the occurrence of pathogenic N. fowleri in the swimming pool.

Amebiasis↗
PubMed1980

Naegleria fowleri from a canal draining cooling water from a factory.

In 1968, a canal draining cooling water from a factory was found to be the source of infection with primary amoebic meningoencephalitis (PAME) (one case). The bed of the canal lined with stone slabs was about 2 m wide, the flow rate of water was approximately 2 m/sec. Average annual water temperatures ranged from 27-30 degrees C. In culture, Naegleria fowleri was not found in the water of the canal, but it was present in scrapings off the canal walls and in its bottom sediment for a length of about 2 km starting at the site of the outlet of the water from the factory. The maximum number of amoebae in 1 liter of the sample was 800 individuals. The present paper discusses the detective efficacy of the culture methods employed, and the epidemiological bearing of the findings.

Eukaryota↗
PubMed1978

Contribution to the problem of the so-called nonpathogenic amoebae in the intestine of man.

Among 10,418 patients of a Prague hospital, a plain infection with intestinal parasitic protozoans was identified in 1,319 persons (12.7%). Of these, 3.5% were infested with Giardia intestinalis, 0.3% with Entamoeba histolytica forma minuta, 5.7% with Endolimax nana. We evaluated the frequency of findings of protozoans in various clinical diagnoses. A statistically significant increase in frequency was recorded for E. nana in diagnoses of eosinophilia, giardiasis, amoebiasis and helminthiasis. A slight increase above the average was found for Entamoeba coli in diagnoses of giardiasis and helminthiasis. Most cases of infection with Entamoeba histolytica were associated with a stay abroad. No increase in the frequency of these protozoans was recorded for patients with diarrhea. An analysis of the results indicated that a nonpathogenic amoeba might participate in the origin of intestinal disorders in man.

Adult↗
PubMed1978

Some further characteristics of the growth of Naegleria fowleri and N. gruberi in axenic culture.

The effects of pH, various viscosity of the medium, changed ratio between the concentrations of dissolved and corpuscular components in the medium, and dissolved inorganic salts on the growth of axenic cultures of Naegleria fowleri and N. gruberi have been studied. The cultures were grown in liquid CALYG and BCS media. The pH optimum was 6.5 for N. fowleri and 6.0--6.5 for N. gruberi. No negative influence on the growth of N. fowleri was observed even at 0.5% concentration of highly viscous methylcellulose, whereas the growth of N. gruberi was distinctly inhibited by more than 0.2% of methycellulose. N. fowleri preferred the osmotorphic and N. gruberi phagotrophic nutrition in the given system of cultivation. The growth of both Naegleria species was inhibited by 0.1 N concentration of sodium chloride and potassium chloride without any significant difference in the tolerance. The inhibitory effect of these salts correlated primarily with the concentration of chloride anion. The ability to grow in a medium with increased viscosity and the preference for osmotrophic nutrtion are, besides the higher temperature optimum determined earlier, further characteristics of the pathogenic species N. fowleri.

Amoeba↗
PubMed1977

Detection of antibody against limax amoebae by means of the indirect haemagglutination test.

A detailed description is given of the procuedure of the indirect haemagglutination test employed in the detection of specific antibodies against pathogenic amoebae of the genera Naegleria and Acanthamoeba. The preservation of all ingredients obtained by lyophilisation provide for a standard pattern of the test. The results of the test with experimentally prepared immune rabbit sera demonstrate an antigenic uniformity of the various isolates of Naegleria fowleri, and their marked difference from Acanthamoeba.

Amoeba↗
PubMed1976

[Diagnostic stain of helminth eggs (author's transl)].

A description is given of a diagnostic method for the staining of eggs and larvae of intestinal helminth in smears of both fresh and fixed stool samples. The contents of the eggs and larvae stain red, the background various shades of blue. The most contrasting staining was obtained with thin-walled eggs.

Amido Black↗