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Biomedical subjects

L Concheiro

Publications and source records attributed to L Concheiro.

36 records · Page 2Linked to original sources
PubMed1987

Plasma protein and red cell enzyme groups in Galicia (north west Spain).

Galactose-1-phosphate uridyl transferase (GALT), esterase D (EsD), and plasminogen (PLG) phenotypes were determined by isoelectric focusing in thin-layer polyacrylamide gels (PAGIF) in a random sample from Galicia. Haptoglobins (Hp) were determined by conventional electrophoresis. The following gene frequencies were observed: for GALT: GALTN: 0.930; GALTD1: 0.044; GALTD2: 0.025; for EsD: EsD1: 0.874; EsD2: 0.104; EsD3: 0.021; for PLG: PLG1: 0.800; PLG2: 0.199; for Hp: Hp1: 0.426; Hp2: 0.573. Population data results of all electrophoretic markers typed until now in Galician population are also included.

Blood Proteins↗
PubMed1987

Alpha-1-antitrypsin (Pi) subtypes in the Spanish Basque provinces.

Alpha-1-antitrypsin subtypes were studied in resident (644) and native (222) individuals from the Spanish Basque Country. The gene frequencies were similar to those in other Spanish populations but the isolated valley of Arratia deviated significantly with increased frequencies of the M2 and M3 alleles and a decrease of the M1 allele.

Alleles↗
PubMed1987

Isoelectric focusing patterns of some mammalian keratins.

An isoelectric focusing method followed by silver staining has been used for the study of keratins from a total of 97 individuals belonging to 17 families and 39 species. The method distinguishes perfectly between two different animal species. In addition, there are often considerable differences between breeds and even occasionally slight individual differences which in no way impede the identification of a particular species as such.

Animals↗
PubMed1983

Distribution of the Pi, TfC, and Gc subtypes in Galicia (North West Spain).

Alpha 1-antitrypsin (Pi), Gc, and TfC subtypes were determined by isoelectric focusing in thin layer agarose (AGIF) and polyacrylamide gels (PAGIF) in a total of 480 individuals from Galicia. The following gene frequencies were observed: for Pi:PiM1:0.660; PiM2:0.115; PiM3:0.060; PiS:0.149; PiZ:0.009; PiF:0.005; PiI:0.001; for Gc:Gc1S:0.572; Gc1F:0.120; Gc2:0.308; for TfC: TfC1:0.778; TfC2:0.180; TfC3:0.041; TfC6:0.001. A rare variant TfC6-2 was found and the intrafamilial distribution of the TfC6 allele studied. The use of these systems for forensic purposes and the peculiar distribution of some of their alleles in the Galician population are discussed.

Adult↗
PubMed1982

PGM1 subtypes in Galicia (NW Spain).

A total of 1,086 individuals from Galicia have been typed for red cell PGM1 using isoelectric focusing in polyacrylamide and agarose gels. The frequency of the four alleles at the PGM1 locus was found to be PGM11+ : 0.6211, PGM11- : 0.1137, PGM12+ : 0.2109, and PGM12- : 0.0543.

Blood Protein Electrophoresis↗
PubMedDate not supplied

A silver staining method for the detection of polymorphic proteins in minute bloodstains after isoelectric focusing.

A silver staining method has been developed to study polymorphic proteins in bloodstains after isoelectric focusing. This method is highly sensitive and permits the detection of polymorphic proteins (i.e. alpha 1-antitrypsin, Gc and Tf C subtypes) in bloodstains as small as 0.2 microliter or less. The method is simple and reproducible and can be used after immunofixation. Blood stains can be identified after longer storage periods than is possible by using conventional staining methods.

Blood Proteins↗
PubMedDate not supplied

The isoelectric focusing of keratins in hair followed by silver staining.

An isoelectric focusing method followed by silver staining has been developed for the study of keratins which is as effective as two-dimensional electrophoresis and fluorography for hair species identification. Hair from dogs, rabbits, horses, cows, guinea-pigs, donkeys, sheep and cats were successfully identified. Narrow pH ranges were used to observe heterogeneity in human hair. Although this heterogeneity may be affected by environmental conditions, it may be of use in criminalistics.

Animals↗
PubMedDate not supplied

Application of pelletized sodium borohydride in the spectrophotometric determination of arsenic.

The spectrophotometric determination of arsenic using sodium borohydride in pellet form, with excipients as the reducing agent and arseine former, has been studied. The use of pelletized sodium borohydride allows the formation of arsenic diethyldithiocarbamate and enables an accurate application of the method without the use of other reagents. The gradual reducing action of the pelletized sodium borohydride, together with the release of hydrogen thus forming arsine with As(III), is the main advantage of this procedure. The arsine thus formed bubbles through the chloroform solution of silver diethyldithiocarbamate to form the complex that allows the determination of the arsenic by visible spectrophotometry.

Animals↗
PubMedDate not supplied

Application of derivative spectroscopy to the determination of plasma theophylline in the presence of phenobarbital.

A rapid, simple, and sensitive procedure for the determination of theophylline in plasma using second derivative spectroscopy is described. Second derivative spectra of plasma extracts containing theophylline in concentrations ranging from 2 to 20 micrograms/mL were studied. The method is suitable for the measurement of plasma theophylline in patients treated for cardiorespiratory disorders. Interference from other compounds such as phenobarbital can be avoided.

Chemical Phenomena↗