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L Contu

Publications and source records attributed to L Contu.

105 records · Page 6Linked to original sources
PubMedDate not supplied

Expression of ATP7B in normal human liver.

ATP7B is a copper transporting P-type ATPase, also known as Wilson disease protein, which plays a key role in copper distribution inside cells. Recent experimental data in cell culture have shown that ATP7B putatively serves a dual function in hepatocytes: when localized to the Golgi apparatus, it has a biosynthetic role, delivering copper atoms to apoceruloplasmin; when the hepatocytes are under copper stress, ATP7B translocates to the biliary pole to transport excess copper out of the cell and into the bile canaliculus for subsequent excretion from the body via the bile. The above data on ATP7B localization have been mainly obtained in tumor cell systems in vitro. The aim of the present work was to assess the presence and localization of the Wilson disease protein in the human liver. We tested immunoreactivity for ATP7B in 10 human liver biopsies, in which no significant pathological lesion was found using a polyclonal antiserum specific for ATP7B. In the normal liver, immunoreactivity for ATP7B was observed in hepatocytes and in biliary cells. In the hepatocytes, immunoreactivity for ATP7B was observed close to the plasma membrane, both at the sinusoidal and at the biliary pole. In the biliary cells, ATP7B was localized close to the cell membrane, mainly concentrated at the basal pole of the cells. The data suggest that, in human liver, ATP7B is localized to the plasma membrane of both hepatocytes and biliary epithelial cells.

Adenosine Triphosphatases↗
PubMedDate not supplied

Heterogeneity of the CD8 lymphocytes in healthy and HIV 1 infected subjects.

CD8 lymphocytes have been subdivided on the basis of Leu2 antigen cell surface density and coexpression of Leu4 and Leu11 antigens in two categories: Leu2bright and Leu2dim. Some CD8 lymphocyte phenotypes present in small percentages in some subjects do not come under these subsets. Flow cytometry analysis moreover shows that the distribution of fluorescence intensity tends to aggregate in most subjects in three distinct spots, here called Leu2HD, ID, and LD. The first, defined by fluorescence levels higher than 450, corresponds to the area of the Leu2+ Leu4+ lymphocytes that express the Leu7 antigen. These cells are all Leu11- and reach remarkably higher percentages in subjects with HIV 1 infection. They are totally included in the area of the Leu2bright cells, but their lowest level of fluorescence is higher. Our data seem to indicate a greater phenotypic homogeneity for these cells. The Leu2LD lymphocytes included in fluorescence levels lower than 175 are all Leu4-Leu11+ and 30% coexpress the Leu7 antigen. They are included in the fluorescence area of the Leud2dim cells, but reach medially lower fluorescence levels. The subset named LeuID has a mean fluorescence ranging between 175 and 450 and includes cells of the Leu4+ 11- and Leu4-11+ phenotypes. Moreover, HIV positive subjects exhibit very low percentages of Leu4+ 11+ and Leu4-11- cells. It is interesting to note that the Leu7 antigen density on the CD8 cell surface is highest for the Leu2HD lymphocytes and diminishes proportionally to the Leu2 antigen density in the Leu2ID and Leu2LD lymphocytes.(ABSTRACT TRUNCATED AT 250 WORDS)

Antibodies, Monoclonal↗
PubMedDate not supplied

Unrelated bone marrow transplantation in a Wiskott-Aldrich syndrome patient sharing two HLA-extended haplotypes with the donor.

We report a case of BMT from an unrelated donor (MUD) in a patient affected by Wiskott- Aldrich syndrome (WAS). The donor-recipient pair was completely identical for two HLA-extended haplotypes. The conditioning regimen consisted of Bu 14 mg/kg followed by CY 200 mg/kg. GVHD prophylaxis was carried out with CsA plus short-term MTX. Allogeneic engraftment was obtained without any signs of acute or chronic GVHD. At fifteen months from the transplant the patient was in an excellent clinical condition. This case confirms the role of BMT from MUD in WAS, and suggests that complete donor-recipient identity for two entire HLA-extended haplotypes is a particularly favorable immunogenetical condition in this type of transplant.

Adult↗
PubMedDate not supplied

[HL-A system in psoriasis; study of 31 families (author's transl)].

31 families with at least two psoriatic members, including sometimes three generations (4 families) have been studied looking for HL-A markers. They include 80 affected (45 females, 35 males) and 75 healthy persons. BW 17 has been found present in 58 p. 100 of the unrelated patients, versus 7 p. 100 only in the normal population (p less than 10-9). Relative risk (R.R.) for the people bearing BW 17 is 38.34. Increase of B 13 is slight and non significant. BW 16, BW 37, BW 27 are within normal range. B 12 (R.R. :0.36) seems to have a protecting effect. B 8, B 14 are also decreased. Study of way of genetic transmission favours existence of a dominant gene of susceptibility frequently associated with BW 17 and particularly with haplotype A 1, BW 17. But other genetic or environmental factors may also play a role.

Adolescent↗