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L D McGill

Publications and source records attributed to L D McGill.

12 recordsLinked to original sources
PubMed1990

Organ-selective switching of 3-methylindole toxicity by glutathione depletion.

A high dose (550 mg/kg) of 3-methylindole (3MI) specifically damaged pulmonary tissue in Swiss-Webster mice without causing any hepatic or renal necrosis. When a glutathione depleter, L-buthionine-(S,R)-sulfoximine (BSO, 1.0 mmol/kg), was administered to mice 3 hr before a low dose of 3-methylindole (75 mg/kg), significant renal damage was observed by histopathological examination after 4 hr. The nephrotoxicity occurred without any observable pathological damage to lung tissues. Increased doses of BSO caused dose-dependent increases in renal toxicity. A low dose of BSO (1.0 mmol/kg) caused no depletion of renal glutathione levels, a large depletion of hepatic glutathione levels (60% of control values), and much larger increases in covalent binding of [methyl-14C]3-methylindole to renal tissues (3.4-fold) than to hepatic tissues (1.5-fold) or pulmonary tissues (2.1-fold). No evidence of hepatic or pulmonary histopathological damage was observed at any dose of BSO with 75 mg/kg 3MI. These results indicate that a shift in organ selectivity of 3MI-induced toxicity from pulmonary to renal sites occurs as a result of glutathione depletion in hepatic tissues. The production of a toxic metabolite in the livers of glutathione-depleted mice that is circulated to susceptible renal cells may be the mechanism of this interesting organ-selective shift in toxicity of 3MI.

Animals↗
PubMed1981

Determinants of pannus formation in long-surviving artificial heart calves, and its prevention.

In a group of 76 calves maintained with Jarvik-5 and Jarvik-7 artificial hearts, pannus formation in the atrial location was evaluated retrospectively using a grading system based on measurement of the pannus tissue. Pannus was present in 87% of the calves surviving one to 7 mos with an old-style inflow quick-connect design utilizing polyester felt surfaces. This design had a suboptimal hemodynamic flow path. Using a new design of quick-connect with an improved hemodynamic flow path and a Biomer blood-contacting surface, pannus occurred only in 17% of calves surviving one to 9 mos. Furthermore, the pannus formation with the new design was mild in the 2 cases where it occurred, whereas the pannus formation in animals with the old cuffs was frequently severe. One calf survived 9 mos with no pannus formation utilizing our new quick-connect system, and we conclude that pannus is no longer a problem in our experiments. We also reviewed the relation of pannus to anticoagulant regimens and found that Persantine, Coumadin, and aspirin were not effective in preventing pannus when the inflow cuff design was conducive to its development.

Animals↗
PubMed1979

Comparison of results using electron microscope, immunodiffusion and fluorescent antibody analyses to detect rotavirus in diarrheic fecal samples of calves.

Seventy-nine diarrheic calf fecal samples were examined by electron microscopy, immunodiffusion and the fluorescent antibody technique for the presence of rotavirus (reovirus-like agent). Thirty-eight (48%) of the samples were positive by electron microscopy, 59% by immunodiffusion and 20% positive by fluorescent antibody technique analyses. Another 9% were suspect-positive by fluorescent antibody technique. Chymotrypsin treatment of the fecal samples increased the ease of observing the viral particles by electron microscopy and also intensified the immunodiffusion arcs obtained. Immunodiffusion analyses using specific antisera to the virus would appear to be a practical method of detecting rotavirus in diarrheic fecal samples.

Animals↗
PubMedDate not supplied

Thermal injury by in vitro incision of equine skin with electrosurgery, radiosurgery, and a carbon dioxide laser.

Freshly harvested equine skin incised with an electrosurgical unit, a radiosurgical device, or a carbon dioxide (CO2) laser was examined by light microscopy to determine the extent of thermal injury caused by each instrument. There was no significant difference between the thermal injury caused by the electrosurgical unit in the pure-cut mode and the CO2 laser in the superpulse mode, or between the electrosurgical unit and the radiosurgical device in the fully filtered cut mode. However, thermal injury caused by the CO2 laser was significantly less than that caused by the radiosurgical device. The amount of thermal injury in this in vitro study was similar to that found in vivo with other species.

Analysis of Variance↗