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L DIENES

Publications and source records attributed to L DIENES.

At least 19 recordsLinked to original sources
PubMed1964

DEVELOPMENT OF L-TYPE GROWTH IN NEISSERIA GONORRHOEAE CULTURES.

Dienes, Louis (Massachusetts General Hospital, Boston, Mass.), Bojana M. Bandur, and Sarabelle Madoff. Development of L-type growth in Neisseria gonorrhoeae cultures. J. Bacteriol. 87:1471-1476. 1964.-Strains of Neisseria gonorrhoeae (22) were studied immediately after isolation from patients. After 24 to 48 hr of incubation, the cocci in the cultures of several strains grew spontaneously into large bodies which later autolyzed. Usually, a secondary growth of cocci started in the autolyzed colonies. Microscopy of the cultures suggested that the cocci were reproduced inside the large bodies or that they originated from tiny L-type colonies. When exposed to penicillin or glycine, all viable cocci grew to large bodies, and after inoculation into the medium many large bodies started to produce L-type colonies. These usually remained small, but their morphology and growth properties corresponded to the early type of L growth. Typical L-type colonies with the usual appearance and structure were observed in two cases, but further cultivation in transfer was not successful.

Bacteriological Techniques↗
PubMed1963

L FORMS ISOLATED FROM A STRAIN OF SERRATIA.

Bandur, Bojana M. (Massachusetts General Hospital, Boston) and Louis Dienes. L forms isolated from a strain of Serratia. J. Bacteriol. 86:829-836. 1963.-In an L culture isolated from a strain of Serratia, two types of colonies developed, one (S-1) similar to the usual L colonies with the centers embedded in the agar and the other (S-2) similar in gross appearance to the usual bacterial colonies. The S-2 colonies consisted almost exclusively of large bodies and were produced by the direct multiplication of the large bodies. This occurred by enlargement, deformation, and segmentation. The organisms in the two types of L colonies were equivalent, and the type of growth was determined by environmental influences. The high viability of the cultures and the relative lack of autolysis permitted the study of the reproductive processes and the resulting growth under varying conditions. The basic reproductive process, as in other L forms and pleuropneumonia-like organisms, seemed to be the multiplication of small granules, either free or in small or large aggregates enclosed in a common envelope. It was possible to observe clearly the growth of small granules from the large bodies.

Agar↗
PubMed1957

Examination of the L forms of group A streptococci for the group-specific polysaccharide and M protein.

Two strains of L forms of group A streptococci were examined for group-specific polysaccharide and found to lack this substance. One of these was found to make a substance that had several properties in common with M protein. It is suggested that the absence of the cell wall polysaccharide is responsible for the lack of rigidity of the L form and that the L form of this species closely resembles protoplasts as prepared from other species.

Cell Wall↗