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L Daehlin

Publications and source records attributed to L Daehlin.

41 records · Page 3Linked to original sources
PubMed1983

The acute effect of oestrogens on testosterone production appears not to be mediated by testicular oestrogen receptors.

Scatchard binding analysis was performed to measure the cytoplasmic oestrogen receptor in the testis of rats. After treatment of rats with the antioestrogen tamoxifen no oestrogen receptor binding was found in testicular low speed supernatant between 12 and 96 h after treatment. Such tamoxifen-treated rats were used to study the acute effect of oestrogens on testosterone secretion, both in vivo and in vitro. Injection of oestradiol benzoate (50 microgram, 24 h prior to experiment) resulted in a significant depression of basal and LH-stimulated plasma testosterone levels in control rats and this effect was unchanged in tamoxifen-pretreated rats. In vitro, oestradiol-17 beta also inhibited the LH-induced rise in testosterone secretion by isolated testicular interstitial cells. This inhibition was not affected if the rats had been pretreated with tamoxifen. These results indicate that the inhibitory effects of exogenous oestrogens on testicular testosterone production are probably not mediated by the oestrogen receptor.

Animals↗
PubMed1982

Acute appendicitis during the first three years of life.

49 children younger than 3 years old and treated for acute appendicitis were investigated retrospectively. The symptoms of this age group are general and diarrhea is not uncommon. Examination of the urine including microscopy should always be performed. The white cell count in peripheral blood is of doubtful value in diagnosing acute appendicitis. A roentgenographic examination of the abdomen was helpful in 78% of the patients studied. The correct diagnosis was delayed in 43% of the cases and this extended the observation time by 2.9 +/- 2.4 days. The appendix was perforated in 79%, complications occurred in 18% and there was no mortality. A high degree of alertness seems essential for the early diagnosis of acute appendicitis in this age group.

Acute Disease↗
PubMedDate not supplied

Thanatogen expression during involution of the rat ventral prostate after castration.

After castration the rat ventral prostate undergoes regression. This process occurs due to the induction of apoptosis, or active cell death, in the epithelial cells of the gland. Several genes, including TRPM-2, (testosterone repressed prostate message), RVP.1, fos, and myc, have been shown to be induced in the prostate during this process. We have investigated the expression of several other genes that may be associated with apoptosis, including tissue transglutaminase (TGase), poly(ADP)ribose polymerase (PARP), and heat shock protein 27 (Hsp27). Northern hybridization has been used to determine the steady-state mRNA levels of these genes in the ventral prostate after castration, and the time course of induction has been compared to the changes in the steady-state levels of prostate steroid binding protein (PSBP), alpha-tubulin, and TRPM-2 mRNAs. The results show that the mRNAs for PARP, transglutaminase, and Hsp27, in addition to TRPM-2, are induced by androgen ablation in the rat ventral prostate and reach maximum levels between days 3 and 4 after castration. Using in situ hybridization we have established that these genes are expressed in the epithelial cells of the prostate that are known to undergo active cell death; this result suggests that their gene products may be required in the dying cells to ensure that the biochemical and morphological processes of apoptosis are completed appropriately.

Androgen-Binding Protein↗
PubMedDate not supplied

Low-frequency mutation of Ha-ras and Ki-ras oncogenes in transitional cell carcinoma of the bladder.

UNLABELLED: BACKGROUND, PATIENTS AND METHODS: The objective was to study the frequency of mutations of Ha-ras and Ki-ras oncogenes in bladder tumours. Transitional cell tumours of the bladder from 55 patients were subjected to analyses of Ha-ras and Ki-ras oncogenes using a variety of techniques including sequencing to detect mutations. RESULTS: Two tumours (4%) exhibited mutation of the Ki-ras oncogene, both tumours were fast growing and invasive. We did not detect Ha-ras mutation in any of the samples. Nineteen tumours (35%) with established invasion of the detrusor muscle, did not reveal any mutation. CONCLUSION: Analyses of ras oncogenes seem to be of limited value for the biological assessment of transitional cell carcinomas.

Aged↗