Biomedical subjects

L Daugeliene

Publications and source records attributed to L Daugeliene.

5 recordsLinked to original sources

PubMed2000

Transient ischemic injury in the rat retina caused by thrombotic occlusion-thrombolytic reperfusion.

PURPOSE: To establish a clinically relevant model of transient retinal ischemia by thrombotic occlusion-thrombolytic reperfusion of the central retinal artery of the rat. METHODS: Thrombus was photochemically induced in the central retinal artery by the combination of intravenous injection of photo-sensitive dye, rose bengal, and green laser irradiation focused on the artery. Transient retinal ischemia for 60 minutes was achieved by a subsequent systemic administration of tissue-type plasminogen activator to reperfuse the occluded vessel. Samples of retinas were excised from the animals killed 3, 9, 12, 24, 48, and 78 hours after the reperfusion. The experimental data were processed using the TdT-dUTP terminal nick-end labeling (TUNEL) method to detect apoptotic cells. RESULTS: The transient retinal ischemia caused time-sequential apoptotic changes in the retinal cells as evaluated by counting the number of TUNEL-positive cells. The most remarkable changes occurred in the central area of retina, and further on the sections taken 24 hours after reperfusion. The peripheral area was less affected, and the outer nuclear cell layer was almost unaffected throughout the observation period. CONCLUSIONS: The proposed method to cause retinal transient ischemia is highly reproducible, and it is easy to simulate the progress and topographical distribution of retinal changes observed in the clinical cases of central retinal arterial occlusion and its subsequent thrombolytic reperfusion. This may provide a useful tool for constructing the effective thrombolytic strategies against the central retinal arterial occlusion and for evaluating the effects of neuroprotective agents.

Animals↗

PubMed1999

Risk factors for visual field damage progression in normal-tension glaucoma eyes.

BACKGROUND: This study was carried out to evaluate intraocular or systemic factors associated with the visual field damage progression in eyes with normal-tension glaucoma (NTG). PATIENTS AND METHODS: Forty-seven NTG eyes with a minimum follow-up of 5 years were enrolled into the retrospective study. A stepwise regression analysis was performed to correlate the visual field damage progression, expressed as the mean deviation (MD) change per year, with several independent clinical factors including age, history of disc hemorrhage, initial MD, mean intraocular pressure (IOP), peak IOP, diurnal fluctuation of IOP, presence of a beta zone of peripapillary atrophy, and use of Ca(2+)-channel blockers. RESULTS: Statistical analysis revealed that non-use of Ca(2+)-channel blockers (P = 0.01), peripapillary atrophy (P = 0.03) and disc hemorrhage (P = 0.04) were associated with visual loss progression. CONCLUSIONS: Risk factors unrelated to IOP were suggested to be associated with progression of visual field loss. Systemic use of Ca(2+)-channel blockers has a favorable effect on visual field prognosis in NTG eyes.

Adult↗

PubMed1998

An image analysis study of cataract development after trabeculectomy with mitomycin C.

We prospectively measured quantitative changes in the crystalline lens after trabeculectomy with mitomycin C. Twenty-four eyes in 24 trabeculectomy cases were consecutively enrolled in the study. The enrollment criteria consisted of: phakic eye, no history of intraocular surgeries and no corneal opacification. The transparency of the lens was measured and analyzed by EAS-1000 preoperatively, then 1 and 3 months postoperatively. Changes occurred primarily in the anterior part of the lens, especially in the anterior subcapsular region. The multivariate regression analysis demonstrated an association of cataract development with age, myopic refraction, difference in preoperative and postoperative IOP and systemic hypertension.

Adult↗

PubMedDate not supplied

Effect of trabeculectomy on visual field in progressive normal-tension glaucoma.

The purpose of this study was to investigate the effectiveness of surgical treatment on the visual field in normal-tension glaucoma (NTG) eyes, and associated factors for visual field progression. Thirty-two patients (32 eyes) were enrolled in this retrospective study. Stepwise regression analysis was performed to correlate the visual field change with several clinical factors, such as intraocular pressure (IOP), and several IOP-unrelated parameters. Surgical reduction of IOP helps in preventing the progression of visual field damage in NTG eyes. The stepwise analysis revealed that better visual field prognosis was associated with higher preoperative IOP (P = 0.006) and the absence of diabetes mellitus (P = 0.04). The functional outcome in the lower IOP group was better when the patients were using Ca2+-channel blockers (P = 0.08); it was worse if disc hemorrhage appeared (P = 0.02). In conclusion, both IOP-related and unrelated factors are associated with visual field damage progression in NTG eyes. The functional prognosis in the eyes with low IOP depends on IOP-unrelated factors.

Adult↗

PubMedDate not supplied

Cataract development after trabeculectomy with mitomycin C: a 1-year study.

PURPOSE: To measure cataract changes quantitatively in the crystalline lens after trabeculectomy with mitomycin C and to identify associated clinical factors. METHODS: Forty-one consecutive trabeculectomy patients (41 eyes) were enrolled in this prospective study. The enrollment criteria were: phakic eye, no history of intraocular surgeries or inflammation, and no corneal opacification. The transparency of the lens was measured and analyzed by an anterior segment analysis system preoperatively and 1, 3, and 12 months postoperatively. Cataract development was correlated with clinical factors using a multivariate regression analysis. RESULTS: During the first postoperative month, changes occurred primarily in the anterior segment of the lens, but later, deeper lenticular areas also started to show an increase in light scattering intensity. Multivariate regression analyses demonstrated that some clinical factors were associated with cataract development. CONCLUSIONS: Slight cataractous changes develop after trabeculectomy with mitomycin C as early as 1 month postoperatively, and they gradually increase in extent and in intensity during the next 11 months.

Adult↗