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Biomedical subjects

L Devriese

Publications and source records attributed to L Devriese.

13 recordsLinked to original sources

Induction of cross-protection in mice against dolphin Erysipelothrix rhusiopathiae isolates with a swine commercial vaccine.

Erysipelothrix rhusiopathiae is well known to cause disease in dolphins. This disease occurs either in an peracute way, leading to mortality even before clinical signs are observed or in a sub-acute way, characterized by rhomboidal skin lesions, that can be treated with penicillin or its derivatives. Commercial swine vaccines, containing inactivated serotype 2 strains, are currently used for vaccination but it is not known whether these vaccines induce protection against E. rhusiopathiae isolates from dolphins. In the present study, it was demonstrated in a mouse model that vaccination with a commercial swine vaccine (Eurovac Ery, Eurovet, Belgium) containing inactivated serotype 2 E. rhusiopathiae strains induced protection against challenge with three E. rhusiopathiae isolates from dolphins. The duration of the protection varied, depending on the challenging isolate, between 8 and >23 weeks. There was however no positive correlation between the amount of antibodies at the moment of challenge and the observed protection. In conclusion, vaccination trials in mice indicate that commercial serotype 2 swine Erysipelothrix vaccines induce protection against erysipelas caused by dolphin pathogenic isolates.

Animals↗

Effects of Staphylococcus aureus mastitis after endotoxin application on milk yield and composition during subsequent lactation of guinea-pigs.

The effects of Staphylococcus aureus mastitis on milk yield and composition throughout subsequent lactation in lactating guinea-pigs and the role of endotoxin pretreatment on these phenomena were investigated. Primiparous lactating guinea-pigs were intramammarily inoculated with sterile saline (group 1), S. aureus strain UC 6097 (group 2), or with S. aureus UC 6097 after endotoxin pretreatment (group 3). Clinical signs and survival rate were monitored. During the second lactation, daily milk yield was measured and milk composition was determined. In primiparous inoculated guinea-pigs, moderate (group 3)-to-severe acute mastitis (group 2) was produced. During subsequent lactation, milk yield in the control group peaked on day 5 and then decreased. Concentrations of Na+ and Cl- in milk, and concentrations of fat, gradually increased, but lactose and K+ decreased. After an early decrease, NAGase in milk increased towards the end of lactation. Except for higher NAGase concentrations in group 3, milk yield and composition during the second lactation did not differ significantly between the mastitis and the control groups. Endotoxin pretreatment only plays a role in the determination of the severity of the infection.

Animals↗

Actinobacillosis in bovine caesarean sections.

An infection with Actinobacillus lignieresii, which was spread by a veterinary surgeon, caused problems after caesarean sections in cows on several farms. The wounds became hard about six weeks after the operation, and a few weeks later small abscesses developed and later the wounds were covered with small and large granulomas. The general health of about 20 per cent of the affected cows was poor and in these cows multiple granulomas could be detected in the abdomen by rectal palpation.

Actinobacillosis↗

The intact fibula.

The notes and radiographs of 281 adult tibial shaft fractures were reviewed. In 17 per cent there was an intact fibula. Delayed union rates were lower when the fibula remained intact, thus confirming that this is a good prognostic indicator. This fracture is caused by less severe trauma than fractures where both bones are broken. It is rare over the age of 30 years.

Adolescent↗

[A trial with attenuated live Salmonella typhimurium vaccine for protection against an experimental Salmonella infection in pigeons].

A live, attenuated Salmonella typhimurium vaccine, available on the market, was administered to six pigeons. The strain of vaccine could not be identified in the droppings of pigeons up to ten days after administration. The pigeons as well as controls were experimentally infected with a Salmonella typhimurium var. copenhagen pigeon strain four weeks after vaccination. Differences in excretion of the var. copenhagen strain and in the appearance of diarrhoea and polyuria between the vaccinated and non-vaccinated pigeons were not detected.

Animals↗

[Antibiotic sensitivity tests (author's transl)].

Sensitivity to antibacterial agents may be studied using quantitative dilution tests and qualitative diffusion tests. There is a strongly felt need for standardized testing methods. Currently, the so-called Bauer-Kirby method appears to be the most suitable test, also for use in veterinary medicine. When this technique is adopted, results are classifid as "sensitive", "resistant" and "showing diminished sensitivity". Interpretations are based on pharmacological, bacteriological and clinical criteria. In some situations, in vitro results have not been sufficiently tested in the field. This would appear to be particularly so in local conditions and treatment.

Ampicillin↗

Newcastle disease: effects of vaccines and vaccination techniques of hemagglutination-inhibition (HI) antibodies and clinical resistance.

Different vaccines (the lentogenic Hitchner-, the avirulent Ulster- and the mesogenic Beaudette strain) and different vaccination schemes (spray, dip, drinking water) were used in 1380 broiler-type chicks on the first day of life and at four weeks. In all these experiments an immunity status was induced sufficient to resist to a strong individual challenge infection, two weeks after the second vaccination. This uniform resistance showed no correlation with the very heterogeneous HI titers which were obtained two weeks after the second vaccination. The titers were, however, strongly dependent both of the vaccine strain used and on the vaccination method. The Beaudette spray-vaccinations provoked the highest titers. The highest post-challenge titers were seen after vaccinations with the lentogenic and avirulent strains and the lowest after-challenge titers were seen when the mesogenic Beaudette strain was used. Birds with high HI titers showed a marked decrease of these HI titers two weeks after challenge, while there was an increase of HI titers in birds with low post-vaccinal titers. It may be concluded that clinical resistance to NCD infection is not directly correlated with high-vaccinal HI titers. Experimental infection by natural route is essential to judge the immune status of fowls.

Animals↗

Bone infection in the bovine appendicular skeleton: a clinical, radiographic, and experimental study.

Four hundred and forty-five bovines with bone infection of the appendicular skeleton were selected for clinical and radiographic assessment. A distinction was made between hematogenous and post-traumatic origin (wound/fracture). Bone infection was classified into four types according to the site of infection: type 1 is metaphyseal and/or epiphyseal osteomyelitis close to the growth plate; type 2 is primary subchondral osteomyelitis mostly accompanied by septic arthritis; type 3 is infectious osteoarthritis with subchondral osteomyelitis implicating that infection in the subchondral bone originates from the infection. Type 4 summarizes bone infections which can not be categorized in the previous groups. Hematogenous osteomyelitis is 3.2 times more frequent than post-traumatic osteomyelitis. Within the different groups, the relation of hematogenous to post-traumatic infection changed significantly. In type 1 infection the ratio was 5/1, in type 2 the ratio was 8/1 and in type 3 it was 3/1. Epiphyseal or metaphyseal osteomyelitis exhibited early radiographic bone changes, whereas with infection eminating from the joint, the bone lesions were detected later, because the bone was infected as a consequence of a primary septic arthritis. In smaller bones, severe and complete bone destruction was often present. Hematogenous bone infection never involved the entire diaphysis. Actinomyces pyogenes was discovered to be the main etiologic agent, whether or not combined with anaerobes. Bone fragments from the metaphysis of young calves were subjected to the effect of pure cultures of different bacteria. Radiographic changes to the structure of the bone were not identified within 2 weeks. Rapid radiographic changes in osteomyelitis cannot be explained by the direct effect of the bacteria on bone tissue in vivo. General infections of the lungs, disorders of the intestines and other internal organs were rarely responsible for osteomyelitis or septic arthritis. Osteomyelitis and infectious osteoarthritis is probably often induced by external and internal traumatic events to joints and bones in cattle.

Actinomycosis↗