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Biomedical subjects

L Doane

Publications and source records attributed to L Doane.

4 recordsLinked to original sources

Enhanced in vitro bone marrow cell migration and T-lymphocyte responses in aged mice given subcutaneous thymic epithelial cell grafts.

Thymic epithelial cell cultures from newborn CBA/J mice were developed and grown on collagen-coated dextran beads. These thymic epithelial cells were transplanted subcutaneously into aged syngeneic mice. After several weeks, enhanced in vitro migration of host bone marrow cells occurred to supernatants prepared from neonatal thymus cultures in thymic epithelial cell grafted mice compared to control aged mice. Percent migration equaled that of migration from control young adult mice. In addition, enhanced T-lymphocyte responses were observed in aged mice given thymic epithelial cell grafts as compared to aged controls.

Aging↗

Augmentation of pre- and post-thymic T-lymphocyte responses in thymic epithelial cell grafted nude mice.

Thymic epithelial (T-E) cell cultures from newborn mice, grown on dextran beads coated with denatured collagen, were injected subcutaneously into athymic "nude" mice. After 8 weeks, enhanced in vitro migration of T-E cell grafted nude mouse bone marrow cells occurred to thymus supernatants prepared from neonatal thymus cultures. Percent migration was equal to the migration of control AKR bone marrow. In vivo T-lymphocyte responses were also significantly enhanced in T-E cell grafted nude mice as compared to controls.

Animals↗

Creating a career ladder for nursing assistants in long-term care.

This article describes the development, implementation, and evaluation of a career ladder for certified nursing assistants in long-term care. A career ladder is an effective way to maximize the use of unlicensed workers without changing the skill mix (eg, no loss of licensed nursing positions) and allow the licensed nurse more time to perform higher-level clinical tasks, such as assessments, patient education, and documentation. Implementation of an unlicensed worker career ladder also can improve nursing assistant retention.

Career Mobility↗