Biomedical subjects
Publications and source records attributed to L Dubuisson.
Electron microscopical study of estradiol-progesterone interrelationships on vaginal epithelium has been performed on 20 days old rats. Mucification or cornification depends on quantitative sequences of two hormones. Cornification is obtained when estradiol is the latest hormone injected, mucification, when sufficient progesterone.
Differentiation of rat vaginal epithelium has been studied in organotypic culture in vitro under different hormonal conditions [evolution in association with ovary or testis, and after injection to rats of progesterone 5 mg. and estradiol 0.1 lambda). Whatever the hormonal preconditioning before the vaginal culture the same, complet cornification occurs.
The seminiferous tubulars of rats testis, subject to the action of Misulban during their foetal life, contain only Sertoli cells and constitute a good model for their study. From the ultrastructural point of view, these cells present several important mitochondria of tubular crest from, an abundant smooth endoplasmic reticulum, a Golgi apparatus well developed, a cytoplasm rich in ribosomes, Lipids droplets and intercellular junctions whose number increases with age. The administration of diethylstilbestrol, which inhibits gonadotropic and androgenic secretions reduces the signs of activity in the Sertoli cells.
She-rats have been treated at the 15th day of gestation with Misulban. The she-rats born from the former ones present an ovarian dysgenesis due to a precocious destruction of the germinal cells by the radiomimetic. These dysgeneses are characterized by the presence of cordal epithelial structures and of stroma. Cells whose cytoplasmic infrastructure is characteristic of a steroidogenesis have been searched on ovaries taken of between 15 days and 6 months. In the absence of follicular organization, they appear only from puberty and are localized particularly, between the epithelial structures, in the conjonctive stroma.
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