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L E Strack

Publications and source records attributed to L E Strack.

4 recordsLinked to original sources
PubMed1984

Posttesticular surface modifications and contributions of reproductive tract fluids to the surface polypeptide composition of boar spermatozoa.

Caput and cauda epididymal fluids were found to be exceedingly rich in the numbers and kinds of polypeptides when analyzed by two-dimensional (2-D) gel electrophoresis. Only a few of the major (Coomassie-stained) and minor (silver-stained) epididymal fluid polypeptides were identified on epididymal sperm plasma membranes (PM) and even fewer identified in ejaculated sperm. The 2-D electrophoretic patterns of caput sperm PM differed little from those of cauda sperm PM. Thus, epididymal transit resulted in relatively minor quantitative and qualitative modifications in sperm PM composition. Seminal plasma showed a few major polypeptides from the cauda epididymal fluid, but the major constituents were those polypeptides from the seminal vesicle secretions. Sperm appear to acquire one acidic high molecular weight polypeptide from either the bulbourethral gland or prostate gland, and another major acidic polypeptide of high molecular weight from the seminal vesicle gland. Numerous neutral and basic low molecular weight polypeptides, originating from the seminal vesicles, adhered tightly to sperm. These were major polypeptides and constituted a substantial percentage of the total PM protein. Thus, major contributions to the sperm PM polypeptide profile occurred at ejaculation. This study did not address loosely bound polypeptides but is the first to analyze, in a comprehensive way, the origins of tightly bound sperm polypeptides from a single species.

Animals↗
PubMed1980

Induced ovulation and conception in locating sows.

Fifty lactating sows were injected with 1,500 IU pregnant mare serum gonadotrophin (PMSG) at an average of 25 days postpartum. Twenty-four of these sows received prostaglandin F2 alpha (PGF2 alpha) 24 hr prior to PMSG. Ninety-six hours after the PMSG injection, 1,000 IU of human chorionic gonadotrophin (HCG) were injected. Artificial insemination was performed at 24 and 36 to 42 hr post-HCG. The PMSG/HCG treatment resulted in pregnancy in 17 of 20 sows slaughtered from 34 to 43 days postbreeding and in 23 of 30 sows allowed to complete gestation. Mean numbers of corpora lutea (33) and viable embryos (15) were counted at slaughter. Litter sizes were averaged (11) for those sows allowed to farrow. Treatment with PGF2 alpha prior to PMSG injection had no effect on conception rates, number of corpora lutea, number of embryos or litter size in the lactating sows. In a second experiment, the same hormone treatments were administered to lactating sows beginning on day 5, 10, 15 or 20 postpartum. Pregnancy rates were 0/10, 2/10, 8/10 and 6/10, respectively (P less than .05, chi-square). At slaughter (30 to 40 days postbreeding), corpora lutea and embryo numbers recorded from pregnant sows were 23.0, 9.5; 31.5, 15.3, and 28.0, 18.8, respectively, for the sows in the day 10, day 15 and day 20 groups. In a third experiment, sows were given PMSG-HCG as previously described on either day 5 (five sows) or day 10 (14 sows) postpartum. Laparotomy of these sows 2 to 5 days postbreeding revealed minimal ovarian responsiveness at day 5, but 43% of the animals responded with multiple ovulations at day 10. The low pregnancy rate seen at day 10 in Exp. 2 may reflect embryonic mortality due to unfavorable uterine environment. We conclude that the PMSG/HCG treatment followed by timed artificial insemination of lactating sows will induce ovulation and coneption as early as 15 days postfarrowing. Pregnancy is thus concurrent with lactation, eliminating the need for early weaning and reducing the interval between successive farrowings.

Animals↗