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L E Swenarchuk

Publications and source records attributed to L E Swenarchuk.

6 recordsLinked to original sources
PubMed1992

Increases in pericellular proteolysis at developing neuromuscular junctions in culture.

To determine whether localized changes in pericellular proteolysis contribute to synapse formation, we examined the degradative actions of developing Xenopus laevis nerve and muscle cells on films of extracellular matrix proteins adsorbed to the glass surface of a tissue culture chamber. Skeletal myocytes, growing neurites, and fibroblasts all removed fluorescent fibronectin and laminin from the culture substratum at regions of close cell-surface contact. In addition, however, motor neurites also displayed a particularly enhanced rate of gelatin elimination at developing neuromuscular junctions. It has already been shown (a) that there is a similar remodeling of organized muscle basal lamina proteoglycan accumulations along the path of nerve-muscle contact and (b) that this is the earliest detectable biochemical change specific to developing neuromuscular junctions. Our observations thus suggest that the establishment of motoneuron-muscle contact leads to a further activation of pericellular proteinases along both the pre- and the postsynaptic surfaces of the developing junction. We therefore consider whether site-specific proteinase-activation cascades could contribute to the inductive signals that direct synaptic differentiation.

Animals↗
PubMed1991

Synaptic differentiation can be evoked by polymer microbeads that mimic localized pericellular proteolysis by removing proteins from adjacent surfaces.

Synaptic differentiation is normally "induced" by regulatory signals that are exchanged only at close contacts between neurites and their predetermined target cells. These signals can, however, be mimicked by contact of either cell with some kinds of polymer microbeads. To find what bead action is responsible for this mimicry, we compared the effects of active and inert microbeads on Xenopus muscle cells developing in culture and on glass-adsorbed films of laminin or fibronectin. Our results show that inductive bioactivity is a property of native polystyrene microbeads that (a) is not dependent merely on bead-muscle adhesion, (b) can be eliminated simply by exposing the beads to inert serum proteins, and (c) correlates closely with the ability of some beads to desorb proteins from adjacent surfaces. Quasi-synaptic differentiation of the muscle surface thus seems to be triggered by the focal removal of peripheral cell surface components, rather than by direct bead interactions with membrane receptors or ion channels or their gradual acquisition of endogenous regulatory substances. Since nerve-muscle interaction also causes an elimination of extracellular matrix proteins from the muscle surface, very early in synapse development, we consider the possibility that the extracellular degradation of peripheral surface components contributes to the transmission of inductive positional signals during synaptogenesis.

Animals↗
PubMed1990

Induction of a specialized muscle basal lamina at chimaeric synapses in culture.

To identify mechanisms that regulate the formation of the neuromuscular junction, we examined the cellular origin of a heparan sulfate proteoglycan (HSPG) that becomes highly concentrated within the synaptic cleft during the initial deposition of the junctional basal lamina. Using cultured nerve and muscle cells from anuran and urodele embryos, we prepared species-chimaeric synapses that displayed spontaneous cholinergic potentials, and eventually developed organized accumulations of acetylcholine receptors and HSPG at the sites of nerve-muscle contact. To determine the cellular origin of synaptic HSPG molecules, these chimaeric junctions were stained with both species-specific and cross-reactive monoclonal antibodies, labeled with contrasting fluorochromes. Our results demonstrate that synaptic HSPG is derived almost exclusively from muscle. Since it has already been shown that muscle cells can assemble virtually all of the known constituents of the junctional basal lamina into organized surface accumulations, without any input from nerve cells, we consider the possibility that the specialized synaptic basal lamina may be generated primarily by the myofibre, in response to another 'inductive' positional signal at the site of nerve-muscle contact.

Ambystoma mexicanum↗