L Engström — Page 6 | PubMed Health

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Deep venous thrombosis after emergency gastrointestinal operations: a pilot study.

In a prospective pilot study, 30 patients over 50 years of age undergoing major emergency gastrointestinal surgery were examined with the 125I-fibrinogen uptake test for postoperative deep venous thrombosis (DVT). Prophylaxis against thrombosis was at that time not used routinely for the emergency patients. The surgeon decided for each patient whether and when prophylaxis with low-dose heparin, 5,000 units twice daily, should be given. DVT was diagnosed in 11 patients (37%; 95% confidence interval 20 to 56%), in five of them bilaterally. No thrombi occurred within seven days in the patients who received low-dose heparin from the day of surgery. Major emergency gastrointestinal surgery carries a high risk of postoperative DVT.

Aged↗

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Abortions in Sweden.

Explore the source record for details and available documents.

Abortion, Legal↗

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Simultaneously synthesized peptides on continuous cellulose membranes as substrates for protein kinases.

Sets of peptides with defined sequences, each on a separate spot, were synthesized simultaneously on continuous cellulose membranes (SPOTs membranes), which were originally designed for epitope studies. The applicability of the membrane-bound peptides as substrates for protein kinases was tested using protein kinase A, protein kinase C and casein kinases I and II as model enzymes. We found that the peptide-membrane complexes can serve as kinase substrates. Our results suggest that membrane-bound peptides offer a new potential for the investigation of substrate specificity of protein kinases. An advantage to this method is that there is no need for substrate identification and separation, which is required with high-volume random peptide libraries. Membrane-bound peptides may even form a basis for kinase assays with peptides lacking multiple basic amino acids, required for separation of the substrates in conventional assays. Problems connected with protein kinase substrate specificity can be investigated in any laboratory using the rapid and inexpensive SPOTs technique, as neither costly apparatus nor special experience in peptide synthesis is necessary.

Amino Acid Sequence↗